Team:Manchester/Parts
PARTS
Main Parts
Agr A+C - K2613741
Part BBa_K2613718 consists of strong promoter CP25 (works in both Lactococcus lactis and Esherichia coli) CITE, RBS (BBa_B0034),coding sequence agrA and agrC and terminators(BBa_B0010 and BBa_B0012). AgrC is a transmembrane receptor histidine kinase, which detects AIP and phosphorylates/activates AgrA. AgrA is the response regulator for AgrC and drives transcription at promoter PII. AgrC and AgrA form a two-component regulatory system, which detects AIP and drives transcription at the PII promoter. In this part, AgrC has a His tag at the N-terminus to aid in protein expression detection.
Agr B+D – K2613743
Part BBa_K2613743 consists of strong promoter pTet, RBS (BBa_B0034), coding sequence agrB and agrD and terminators(BBa_B0010 and BBa_B0012). Expression of agrB and agrD is regulated by TetR. AgrB is an integral membrane protein; AgrD is a short peptide that is transported outside of the cell by AgrB. During transportation, AgrD is covalently modified to produce a small autoinducing peptide (AIP) which can be taken up by cells and induces transcription of genes under control of PII promoter.
Part Table
<groupparts>iGEM18 Manchester</groupparts>