Lab Notes
[Abbreviation notes: 4L = part BBa_K325909 (LuxCDABEG), 2D = part BBa_K325219 (Red firefly luciferase and LRE), 4N = part BBa_K325100 (EPIC firefly luciferase and LRE); Concentration of plasmids are in units of ng/μL (if not mentioned)]
Lab Notes
7/17
- I7 test tubes, 4 monoclone (4L, 2DH, 2DH-5)
- 2D and 4N transformation (2DH,2DH-5)
7/18
- Plasmid extraction for 7 test tubes
- PCR 4L and LuxG (failed)
7/19
- Plasmid extraction
7/21
- Four Agrobacteria transformations for 2*4L, 1*2D and 1*4N
7/25
- Transformed 2*LuxG, 2*4L, 2*2D, 2*4N, totaling up to 9 petri dishes
- 2*LuxG 2*4L 1*2D 1*4N totaling up to 6 test tubes