Results
1. Construction of recombinant plasmid of HCV Core protein
1.1 Codon optimization of HCV core protein
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Fig.1 Part sequence of original (left) or optimized (right) HCV core protein at website http://gcua.schoedl.de/sequential_v2.html
optimized sequence of HCV core gene was showed as follows:
GGTACCATGAGTACCAATCCGAAACCGCAGCGCAAAACCAAACGTAATACCAATCGTCGTCCGGAAGATGTTAAATTTCCGGGCGGCGGTCAGATTGTGGGCGGCGTTTATCTGCTGCCGCGTCGTGGCCCGCGTCTGGGTGTTCGTACCACCCGTAAAACCAGTGAACGCAGTCAGCCGCGCGGCCGCCGTCAACCTATTCCGAAAGATCGTCGCAGTACCGGCAAAGCCTGGGGCAAACCGGGCCGTCCGTGGCCTCTGTATGGTAATGAAGGTCTGGGCTGGGCCGGTTGGCTGCTGAGCCCTCGTGGTAGTCGTCCGAGTTGGGGCCCGACCGATCCGCGTCATCGCAGTCGTAATGTGGGTAAAGTGATTGATACCCTGACCTGTGGCTTTGCAGATCTGATGGGCTATATTCCGGTGGTTGGCGCACCGCTGAGCGGTGCAGCACGCGCAGTTGCACATGGCGTTCGTGTTCTGGAAGATGGTGTTAATTATGCCACCGGCAATCTGCCGGGCTTTCCGTTTAGTATTTTTCTGCTGGCCCTGCTGAGCTGTATTACCGTGCCGGTGAGCGCCCTGCAG