Team:ZJU-China/Attributions

Improve Part
ATTRIBUTIONS 

Welcome! This year, we designed 23 parts, and all of them have been submitted to the iGEM Registry following the Biobricks assembly standard as described by RFC10. See more detailed information on our part pages !


This year ZJU-China consists of 14 members, who can be separated into 2 groups, including the wet-lab group and the dry-lab group. Wet-lab group members have the background in life science and medical science while the dry-lab group members mainly major in engineering and computer science. This year, ZJU-China focused on developing an in-vitro diagnostic device whose idea was first brought out after rounds of brainstorming. After identifying the direction of our project, we always worked together, designing and optimizing our products rounds by rounds accompanied with a continuous process of Human Practice.


Unless otherwise stated, all the experiments, constructs, logos and results documented on our wiki were performed, composed and collected by ourselves.



Lab and modelling support

Sincere thanks to whoever helped us!

Interlab support

Associate Professor Xianyin Zhang provided guidance and permissions on FCM.

Lab support

Associate Professor Fan Yang, Professor Min Wu, Professor Ming Chen helped us maintain our laboratory work!


Professor Neel Joshi, Professor Urartu Seker, Professor Chao Zhong gave us valuable advice on our experiments!


Professor Chao Zhong provided us with the CsgA Knockout Bacteria Strain From E.Coli MG1655.


Professor Bin Su provided guidance and permissions on electrochemical workstation.


Director of Clinical Laboratory in Run Run Shaw Hospital, Jun Zhang, Director of General Surgery Department in Run Run Shaw Hospital, Yifan Wang, Doctor Hong Jin, Doctor Jingjuan Song, Doctor Wei Jin helped us apply our diagnostic device in Run Run Shaw Hospital.


Storage

The chemical and biological reagents are stored in the proper place and suitable temperature. Hazardous or corrosive chemicals are stored separately.

Fig.2 Storage for chemical and biological reagents
Waste

Waste should be classified and disposed of separately.

Safety Apparatus

Our iGEM lab is equipped with safety apparatus such as fire extinguishers, emergency showers, eyewash stations, and first aid kits in case of dire situations.

Fig.3 The fire extinguisher
Fig.4 The fire blanket
Fig.5 The emergency shower
Fig.6 The eyewash equipment
Fig.7 The first-aid case
References

[1] Michelle M. Barnhart Matthew R. Chapman. Curli Biogenesis and Function. Annual Review of Microbiology(2006):131-147

[2] CDC.Recommendations for Prevention of HIV Transmission in Health-Care Settings.MMWR,1987;36

[3] Z Botyanszki, PKR Tay, PQ Nguyen, MG Nussbaumer, NS Joshi.Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers. Biotechnology & Bioengineering, 2015, 112 (10) :2016-2024