Team:JNFLS/Demonstrate

Demonstrate

We developed a biosensor based on the aptamer target on the HCV core protein, using competition rolling amplification. This device could be used for the detection of trace HCV virus in unpaid donation blood test and blood transfusion in clinic, which could shorten the window period of HCV detection.

1. The HCV C-O120 recombinant plasmid worked well

In order to express HCV C protein efficiently, the complete HCV C gene was truncated to 360bp and optimized using software. Fig.1 and Fig.2 illustrated that HCV C-O120 protein was expressed and purified very well, which allowed the following experiments could be done.