Team:Jilin China
ABSTRACT
Many strategies could be used to regulate the gene expression responding to temperature changes in bacterial cells. A well-known class of biological thermoregulatory element are RNA-based thermosensors (RTs), which are located in the 5'-untranslated region of mRNA. RTs could sense temperature shift and influence the mRNA stability or ribosome accessibility, thereby controlling translation efficiency.
However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation in their prospective application. To address this issue, we created a synthetic RNA-based thermosensors (SynRTs) toolkit with different sensing temperatures and different intensities. Futhermore, we built a matched search engine for potential users.
The SynRT toolkit version has been updated from 1.0 to 3.0, and the newest version is composed of four types of SynRTs:
Hot-induced 5'UTR
Human Practices
Model
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