Team:Munich/capsulation.html

Phage Encapsulation

2018/08/30

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	First Test of Encapsulation, Droplets: r=30 µM, polydisperse.

Phage Encapsulation

30/08/2018

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Results: Design of own apparatus and first 3D testprints, filament evaluation (PLA, PMMA).

Phage Encapsulation

2018/09/02

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	First test of 3D print, needs some updates for better fitting, PMMA seems to be best material.

Phage Encapsulation

2018/09/04

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Works better now, better assembly, Droplets look good, but a lot of aggregate, next time more CaCl2 solution.

Phactory purifier

2018/09/04

Participants:	Johann Brenner, Keno Eilers
Protocol:	NAME,
Notes:	No notes.
Results:	Spot test positive for fractions 13-23.

Phage Encapsulation

2018/09/05

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Test of transferring Droplets in Phage Buffer --> white sediment; CaPO4 crystals.

Phage Encapsulation

2018/09/12

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Alginate solution with10 mM MgSO4, different batches with different flow rates and N2 pressure, lack of reproducibility.

Phage Encapsulation

2018/09/13

Participants:	Johann Brenner
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Design and print of new apparatus which holds dispenser needle in place.

Phactory purifier

2018/09/13

Participants:	Johann Brenner, Keno Eilers
Protocol:	NAME,
Notes:	No notes.
Results:	Spot test positive for fractions 10-23.

Phage Encapsulation-stain of capsules with DAPI

2018/09/14 - 2018/09/20

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Test of different flow rates, enhanced reproducibility.

Phage Encapsulation

2018/09/21 - 2018/10/01

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Test of different Alginate/low viscosity Alginate ratio 1.8% Alginate + 0.2%low viscosity gave the best results.

Phage Encapsulation-Sybr Gold stain

2018/10/02

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Sybr Gold stained Phages are clearly visible, next time wash away of un bound Sybr Gold.

Phage Encapsulation-Test of cleaning Phages

2018/10/04

Participants:	Johann Brenner, Keno Eilers
Protocol:	Plaque Assay,
Notes:	No notes.
Results:	Phages (in PBS) were centrifuged with 30 K centrifugal filters and stored in 10mM MgSO4 concentration of phages can be increased.

Phactory purifier

2018/10/04

Participants:	Johann Brenner, Keno Eilers
Protocol:	NAME,
Notes:	No notes.
Results:	Spot test positive for several fractions, positive fractions were concentrated with centrifugal filters.

Phage Encapsulation with Sybr Gold staining

2018/10/06

Participants:	Johann Brenner, Keno Eilers
Protocol:	Alginate Encapsulation
Notes:	No notes.
Results:	Clearly visible fluorescent signal with less background than before.

Test of encapsulated phages in HCl(aq)

2018/10/06

Participants:	Johann Brenner, Keno Eilers
Protocol:	NAME,
Notes:	No notes.
Results:	Phages were monitored over 7h in HCl ph1, microspheres stay the same size over the time.

Test of Phages droplets in SGF and SIF: Phage Encapsulation

2018/10/07 - 2018/10/14

Participants:	Johann Brenner, Keno Eilers
Protocol:	NAME, Encapsulated phages in SGF and SIF
Notes:	No notes.
Results:	Capsules show enhanced stability in SGF and high release of active phages in SIF.