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iGem SDSZ_China 2018 Obtaining Genes
Both fungal and bacterial acetylases have three functional domains- a deacetylase domain, a chitin binding zone, and a low density lipoprotein receptor region. By using the bioinformatics BLAST technique, a sequence highly conserved with known deacetylase domain sequences was searched in the NCBI soft armor gene database.
1 atggcaagag taagatgcgg tagttctctt gccctccttg gcattgtgct gccgagcggg
61 tcaagaggca ggcagtttca gacacgaccg aggatggagc gaaccttcaa gagggaactg
121 tgcaaggata agggcgccgg cgagtggttc cgtctcagcc tcggtgactg tcgcgatgtg
181 atccagtgta ccgacgccgg tcttcaggcg ctgcgttgcc cccacggcct ggccttcaac
241 ctggagcagc agacctgcga ctggaaagcc aacgtcaaga actgcgacag gaaggagaag
301 acgaaggtcg tgaagcctct cttcaacacc gtcgagcccc tttgccagga aaaccagttg
361 gcgtgcggtg acggcacctg tctggatagg caggtcttct gtgatggaaa agaagattgt
421 accgatggtt ctgacgagac cgcatgtgac gtgaaaaatg accccaacag cgctcccatc
481 tgcaacaccg aggactgccg cctccccgat tgctactgct acaacgaccc tagcgagatg
541 ccccacaaca tgaagccttc cgaagtgcct cagatggtca ccatcacttt cgacgatgcc
601 atcaacatca acaacatgga cctgtacgag ctcatcttca agcagcgctt caaccccaac
661 ggctgctcca tcaagtccac cttcttcgtt tctcacaaat acaacaacta caccgccact
721 caggagatgc accgtctggg tcacgagatc gctgttcact ccatcaccca cgcaaacaac
781 gagactttct ggtcccacgc aagcgaagac gagtacgagc gcgaaatggg tggtgcccgc
841 gtcatcatcg aacgcttcgc caacatcacc gaccagtcca tcatcggcat gcgtaatccc
901 ttcctccgtg tgggcggcaa cagccagttc aggatgatgg agaagaacac cttcctgtac
961 gactccacca tcactgcccc cctgtcctcc atgcccctgt ggccctacac cctgtactac
1021 cgcatgcccc acccctgcca tggaaacctc cagaactgcc ccactcgctc cttcgcagtg
1081 tgggagatgg tcatgaacga gatggaccgt cgcgaggaac ccacttacga ggacggcctc
1141 cccggatgcc acatggtcga ttcctgcttc gccaccaagc ccgagcccga gcagttctac
1201 aacttcctgc agaacaactt caaccgccac tacaagtcaa accgcgctcc cttcggtctg
1261 ttcttccact ctgccttcct gaagaacaac cccgacatcc tggacacctt cctctactgg
1321 ctggacgaga ccctgaagaa ccagaaggac gtttacttcg tcaccatgac ccaggtcatc
1381 cagtggatgc aggacccccg ccccgtcggc cagctgaaca actacgaggc ctggaaggag
1441 aagtgcgtcg tcgacggccc acccttctgc tacggcggca acaactgcga gctggacact
1501 gacgagctcc ctggccagac cctccacctg tccacctgca tgcggtgccc caacaattat
1561 ccctggacaa gggacccatt gggcgaggga ttcttctaa