Team:ECUST/Biofilm Remover
Design
“Human Practices is the study of how your work affects the world, and how the world affects the work.” (Peter Carr, Director of Judging) is the comment which gives a clear definition of all Human Practices activities. Therefore, we tried to answer two questions: how can synthetic biology affect the world? And in which way can the world affect Iron Guardian? To answer the former one, we chose to collaborate with different teams and thought the issues we are working on vary from team to team, we all wish and try to utilize what we learn to solve practical problems and make the world a better one. What’s more, we integrated the social influence into our project to answer the latter question. With communication, economic benefit and responsibility as our important focus, we interviewed professors and employees in related industry, and showcased the great economic benefit of our project. By distributing questionnaire and analyzing the data acquired, we managed to explain and improve our project according to the public concerns and suggestions. The new concept of “3+ net” raised by ECUST will also help all teams to create, to reflect, and to improve.
Construct
In order to test the biofilm removal effect of DSPB, we constructed the vector pET28a-DSPB
The plasmid was transformed into E. coli BL21, cultured at 37 °C for 12 h, and the plasmid was extracted and verified by PCR.
Result
In order to test the biofilm removal effect of DSPB, we constructed the vector pET28a-DSPB
The plasmid was transformed into E. coli BL21, cultured at 37 °C for 12 h, and the plasmid was extracted and verified by PCR.
To test the expression of DSPB, we cultured E. coli in LB medium containing 0.1% kan. E. coli was cultured at 37℃ and 220 rpm for all night, then inoculated into fresh medium and cultured until logarithmic phase.Add IPTG to final concentration of 20uM and culture E. coli over night at 25℃. Final OD600=0.437(diluted 16 fold)