Construct
In order to test the biofilm removal effect of DSPB, we constructed the vector pET28a-DSPB
The plasmid was transformed into E. coli BL21, cultured at 37 °C for 12 h, and the plasmid was extracted and verified by PCR.
Result
In order to test the autolysis effect of Lysin, we constructed the vector pET28a-Lysin.
The plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR.
In order to verify whether the cell expression of Lysin can perforate on the plasma membrane, we test the growth curve of recombinant E.coli and check them under the scanning electron microscope.
Recombinant bacteria is cultured by LB medium adding with 0.1% kanamycin till logarithmic phase and induced by IPTG. Then culture solution is transferred into 96-well microtiter plates and measured the light absorption of 600 nm.
