Team:ECUST/Biocide
Description
When the concentration of Fe3+ in the environment is high, it means that the hard skeleton of the microenvironment of the iron bacteria is destroyed and loses protection. At this time, the added bactericide will have a greater killing effect on the bacteria. The bactericide we selected was cecropin AD, a cationic chimeric antimicrobial peptide obtained from cecropin, consisting of the first 11 amino acid residues of cecropin A and the last 26 amino acid residues of cecropin D. It has strong antibacterial activity against Gram-positive and negative bacteria.
Design
Result
We insert fragment of Cecropin AD into vector pET-28a(Figure )
The plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR(Figure).
We verified the expression of Cecropin AD by SDS-PAGE(Figure).