Team:Fudan/Primers
Primers used
Primers
| Serial Number | Oligo Name | Sequence(5to3) | |
|---|---|---|---|
| O1 | pLL7.1-F(S) | GGTCTATATAAGCAGAGCTGG | |
| O2 | pLL7.1-R(S) | AACCGGAACCCTTAAACATG | |
| O3 | LaG16-F(G_pLL7.1) | TGAACCGTCAGATCCGCTAGAATTCGCCACCATGGCCCTGCCCGTGACC | |
| O4 | LaG16-R(G_mNc) | AGTCCAGGATGCTCACGGTCACCTGGGTG | |
| O5 | mNc-F(G_LaG16) | GACCGTGAGCATCCTGGACTACAGCTTCACAGGTGGCGC | |
| O6 | mNc|e-R(G_tTa) | ATCTAGACATCCGCCGGCGCTTGCGGGA | |
| O7 | tTa-F(G_mNc|e) | GCGCCGGCGGATGTCTAGATTAGATAAAAGTAAAGTGATTAAC | |
| O8 | tTa-R(G_pLL7.1) | CCCTCGACGAATTGCCTGCAGGCTACCCACCGTACTCGTCAATTC | |
| O9 | LaG16-R(G-mNc(e)) | CCACACAGGGGCTCACGGTCACCTGGGTG | |
| O10 | mNc(e)-F(G_LaG16) | GACCGTGAGCCCCTGTGTGGGTAGCAACCCCTGCTAC | |
| O11 | mNotch 1 core-PF | ATCCTGGACTACAGCTTCACAG | |
| O12 | mNotch 1 core (extended)-PF | CCCTGTGTGGGTAGCAACC | |
| O13 | mNotch 1 core-PB | CCGCCGGCGCTTG | |
| O14 | LaG16-F(EcoRI-Kozark) | GCTGAATTCGCCACCATGGCCCTGCCCGTG | |
| O15 | LaG16-B(OE | mNotch) | CTGTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGG |
| O16 | LaG16-B(OE | mNCe) | GGTTGCTACCCACACAGGGGCTCACGGTCACCTGGG |
| O17 | mcherry-F(OE | mNotch) | CAAGCGCCGGCGGATGGTGAGCAAGGGCGAG |
| O18 | mCherry-B(UAA-HindIII) | GCTAAGCTTTTACTTGTACAGCTCGTCCATGC | |
| O19 | CMV-F(ala-EcoRI-ala-XbaI) | GCAGAATTCGCATCTAGAGACATTGATTATTGACGAGTTATTAATAGTAATCAATTACG | |
| O20 | LacOi-F(OE | intron) | CGTAATTGTGAGCGGATAACAATTACGTTCGCCCCGTGCCCCG |
| O21 | LacOi-B(OE | intron) | CGTAATTGTTATCCGCTCACAATTACGGGCAACGCAGCGACTC |
| O22 | intron-B(SpeI-ala-HindIII-ala) | GCAAAGCTTGCAACTAGTCTGTAGGAAAAAGAAGAAGGCATGAAC | |
| O23 | LaG16-F(G_pML2-EcoRI-Kozark) | AGTTATTAGGTCCCTCGACGAATTCGCCACCATGGCCCTGCCCGTGACC | |
| O24 | LaG16-R(G_mNC) | GTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGGTG | |
| O25 | LaG16-R(G_mNCe) | GGGTTGCTACCCACACAGGGGCTCACGGTCACCTGGGTG | |
| O26 | mcherry-F(G_mNC) | TGTCCCGCAAGCGCCGGCGGATGGTGAGCAAGGGCGAG | |
| O27 | mcherry-R(HindIII | G_pML2) | AAGTTATATTAAGGGTTCCAAGCTTTTACTTGTACAGCTCGTCCATG |
| O28 | rev_41BB_CD3z9 | 序列缺失 | |
| O29 | hNotch-4168 | CCCTGCCTGGGCGGCAAC | |
| O30 | 5286-hNotch | CCGCCGGCGCTTGCGGG | |
| O31 | tTA-start | CAAGCGCCGGCGG ATGTCTAGATTAGATAAAAGTAAAGTG | |
| O32 | rev_tTA-end-3NotI-2-Hind3-5 | GTTCCAAGCTTAAGCGCTACCCACCGTACTCG | |
| O33 | EGFP_BglⅡ_F | GCAGATCTatggtgagcaagggcga | |
| O34 | O34_EGFP_XbalI_R | GCTCTAGActttacttgtacagctcgtccat | |
| O35 | O35_rtTA_pLL7.0_F | TGAACCGTCAGATCCGCTAGAAGTTTGTACAAAAAAGCAG | |
| O36 | O36_rtTA_EGFP_R | TGGCACTTTTTTTAAATGATTTGCCCTCCC | |
| O37 | O37_rtTa_EGFP_F | GCAAATCATTTAAAAAAAGTGCCACCTGACGTC | |
| O38 | O38_LacI_pLL7.0_R | GCAAATCATTTAAAAAAAGTGCCACCTGACGTC | |
| O39 | O39_lacI_mFF4_ployA_F | TACCGCGGGCCCGGGATCCATTTGACAATTAAAGCTTGAGG | |
| O40 | O40_mFF4_ployA_pCAGop_R | attaataactaGCGCAGCGAGTCAGTGAG | |
| O41 | O41mFF4_polyA_pCAGop_F | ctcgctgcgcTAGTTATTAATAGTAATCAATTACGGG | |
| O42 | O42_pCAGop_pLL7.0_R | CTTGCTCACCATGGTGGCGACTGTAGGAAAAAGAAGAAGG | |
| O43 | O43_mFF4_pll7.0_R | CTTGCTCACCATGGTGGCGAGCGCAGCGAGTCAGTGAG | |
| O44 | O44_TRE_pLL7.0_R | cgcggtaccgtcgactgcagTACCGAGCTCGAAATCTC | |
| O45 | O45_TRE_GAL4_F | TACCGCGGGCCCGGGATCCAAAGTTTGTACAAAAAAGCAG | |
| O46 | O46_GAL4_UAS5_R | TCACATGAGATACAGTCAACTGTCTTTG | |
| O47 | O47_GAS4_UAS5_F | gttgactgtaTCTCATGTGATTACGCCAAG | |
| O48 | O48_UAS5_mCherry_R | cttgctcaccatggtggcgaTGCTTTTTTGTACAAACTTGG | |
| O49 | O49_pLL7.0_mCherry_F | aagcttatcgataatcaacctctggattacaaaatttgtgaaaga | |
| O50 | 050_pLL7.0_mCherry_R | GATATCGAATTGTACCTAGTGGAACCGGAACC | |
| O51 | O51_mCherry_FF4_polyA_F | actaggtacaattcgatatcGAATTCTGCAGTCGACGG | |
| O52 | O52_FF4_ployA_pLL7.0_R | ggttgattatcgataagcttTTTGCCCTCCCATATGTC | |
| O53 | 053_EGFPpoly(A)F | agtttggttagtaccgcgctGCAAATCATTTAAAAAAAGTGCCACC | |
| O54 | 054_EGFPpoly(A)R | cctggagaatgccaccATGGTGAGCAAGGGCGAG | |
| O55 | 055_Bi_TRE F | tgctcaccatggtggcATTCTCCAGGCGATCTGACGG | |
| O56 | 056_Bi_TRE R | cgcggtaccgtcgactgcaggaattcCGCGGTACCGTCGACTGC | |
| O57 | 057_pll7.0.1 F | cagggatattcaccattatcgtttcagacc | |
| O58 | 058_poly(A)F | cgacggtaccgcgggcccggAACTTGTTTATTGCAGCTTATAATG | |
| O59 | 059_poly(A)R | caccatggtggcgaccggtgGCAGTGAAAAAAATGCTTTATTTG | |
| O60 | 060 BiTRE F | ccatggtggcattctccaggGCCTCAGGAGATCTcAATTCtcc | |
| O61 | 061 BiTRE R | cgcggtaccgtcgactgcagaattctaccgagctcgaaatctcca | |
| O62 | 062 pSB1C3-VR | ATTACCGCCTTTGAGTGAGC | |
| O63 | 063 pSB1C3-VF | TGCCACCTGACGTCTAAGAA | |
| O64 | 064 pSB1C3-BP | GAATTCGCGGCCGCTTC | |
| O65 | 065 pSB1C3-BS | CTGCAGCGGCCGCTAC | |
| O66 | O66 EGFP-F(G_pQM6.2) | gcaagagcaagggccaattgGTGAGCAAGGGCGAGGAG | |
| O67 | O67 EGFP-R(G_P2A) | gtcgacccCTTGTACAGCTCGTCCATGC | |
| O68 | O68 P2A-F(G_EGFP) | ctgtacaagGGGTCGACGGGCTCCGGC | |
| O69 | O69 mcherry-R(G_pQM6.2) | ccctttttctggagacaagcttTTACTTGTACAGCTCGTCCATGCCG | |
| O70 | O70 pQM6.2-R(L-P2A) | ggatctggggccggggttctcc | |
| O71 | O71 pQM6.2-F(L-EGFP) | atggtgagcaagggcgaggagc | |
| O72 | O72 alpha-SP-LaG16-F(3-AgeI) | GCAACCGGTGCCACCATGGCCCTGC | |
| O73 | O73 tTa-R(HindIII-3) | GCAAAGCTTCTACCCACCGTACTCGTCAATTCc | |
| O74 | O74 P-minCMV-F | GAATTCGCGGCCGCTTCTAGgaggtaggcgtgtacggtg | |
| O75 | O75 S-minCMV-R | CTGCAGCGGCCGCTACTAGTAggcgatctgacggttcactaaac | |
| O76 | O76 P-pSV40-F | GAATTCGCGGCCGCTTCTAGgtgtgtcagttagggtgtgg | |
| O77 | O77 S-pSV40-R | CTGCAGCGGCCGCTACTAGTAtttgcaaaagcctaggcctccaaaaaag | |
| O82 | O82 SV40NLS-KRAB-F | CCAAAAAAGAAGAGAAAGGTAatggacgccaagagcctg | |
| O83 | O83 BS-F | tactagtagcggccgctgc | |
| O84 | O84 BP-R | ctctagaagcggccgcg | |
| O85 | O85 KRAB-R | caccagccagggttcctc | |
| O86 | O86 mcherry-F | ATGGTGAGCAAGGGCGAGGAGGATAACATG | |
| O87 | O87 pTight-F(S) | AGCTCGTTTAGTGAACCGTCAG | |
| O88 | O88 3 LTR (Delta-U3)-R(S) | GCGTTACTTAAGCTAGCTTGCC | |
| O89 | O89 P2A-F(S) | ACCAACTTCTCCCTGCTGAAG | |
| O90 | O90 mcherry-R(S) | CATGTGCACCTTGAAGCGC | |
| O91 | 091 BP protect | tttctggaattcgcggccg | |
| O92 | 092 BS protect | gccggactgcagcg | |
| O93 | 2* ZF 42-10 operator | GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a GAC GCT GCT c TACTAGTAGCGGCCGCTGCAG | |
| O94 | 2* ZF 54-8 operator | GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a TGG GTG GCA t TACTAGTAGCGGCCGCTGCAG | |
| O95 | 2* ZF 43-8 operator | GAATTCGCGGCCGCTTCTAG a GAG TGA GGA c a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG | |
| O96 | 2* ZF 21-16 operator | GAATTCGCGGCCGCTTCTAG a TTA GAA GTG a a TTA GAA GTG a TACTAGTAGCGGCCGCTGCAG | |
| O97 | 1* ZF 42-10 & 54-8 operator | GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a TGG GTG GCA t TACTAGTAGCGGCCGCTGCAG | |
| O98 | 1* ZF 42-10 & 43-8 operator | GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG | |
| O99 | 1* ZF 54-8 & 43-8 operator | GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG | |
| O100 | 2* ZFHD1 | GAATTCGCGGCCGCTTCTAG TAATGATGGGCG TA TAATGATGGGCGTATAATGATGGGCGTATAATGATGGGCG TA TACTAG | |
| TAGCGGCCGCTGCAG | |||
| O101 | 1*PIR | GAATTCGCGGCCGCTTCTAGGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACTACTAGTAGCGGCCGCTGCAG | |
| O102 | O102 pCMV-F(S) | cgtgtacggtgggaggtctatataag | |
| O103 | O103 pML2-R(S) | acgagattccagcaggtcg | |
| O106 | O106 pSB1C3-BP(L) | tttctggaattcgcggccgcttc | |
| O107 | O107 pSB1C3-BS(L) | gccggactgcagcggccgc | |
| O108 | O108 PIR-F(6-XbaI) | GCCGCTTCTAGagGAAATAGCGCTGTACAGCG | |
| O109 | O109 2*ZFHD1-F(6-XbaI) | GCCGCTTCTAGagTAATGATGGGCGTATAATGATGG | |
| O110 | O110 CD8-alpha-SP-F(G_pML2) | gatccgctagcgctaccggtgccaccATGGCCTTACCAGTGACCGCCTTG | |
| O111 | O111 alpha-GPC3-R(G_mNce) | acacagggTGGTCGCGGCGCTGGCGT | |
| O112 | O112 mNce-F(G_alpha-GPC3) | cgcgaccaCCCTGTGTGGGTAGCAACC | |
| O113 | O113 tTa-R(G_pML2) | gttatattaagggttccaagcttCTACCCACCGTACTCGTCAATTC | |
| O114 | O114 alpha-GPC3-R(G_mNc) | TCCAGGATTGGTCGCGGCGCTGGCGT | |
| O115 | O115 mNc-F(G_alpha-GPC3) | cgcgaccaATCCTGGACTACAGCTTCACAG | |
| O116 | O116 CD8-alpha-F2(G_pML2) | GATCCGCTAGCGCTACCGGTGCCACCATGGCCCTGCCCGTGACC | |
| O117 | O117 CD8-alpha-SP-F(G_pML2) | cgtcagatccgctagcgctaccggtGCCACCATGGCCCTGCCCG | |
| O118 | O118 KRAB-R(G_pML2) | aagttatattaagggttccaagctttcaCACCAGCCAGGGTTCCTC | |
| O119 | O119 mNc|e-R(G_Gal4DBD) | gcagcttcatCCGCCGGCGCTTGCGGGA | |
| O120 | O120 Gal4DBD-F(G_mNc|e) | gcgccggcggATGAAGCTGCTGAGCAGCATC | |
| O121 | O121 Gal4DBD-R(G_SV40NLS) | tacctcctcctccCACTGTCAGCTGCCGCTG | |
| O122 | O122 SV40NLS-F(G_Gal4DBD) | gctgacagtgGGAGGAGGAGGTAGTCCAAAAAAG | |
| O123 | O123 mNc|e-R(G_PIP) | cccttgacatCCGCCGGCGCTTGCGGGA | |
| O124 | O124 PIP-F(G_mNc|e) | gcgccggcggATGTCAAGGGGGGAGGTTAG | |
| O125 | O125 PIP-R(G_SV40NLS) | tacctcctcctccGGCCTGCTCCACCATAGC | |
| O126 | O126 SV40NLS-F(G_PIP) | ggagcaggccGGAGGAGGAGGTAGTCCAAAAAAG | |
| O127 | O127 mNc|e-R(G_ZF21-16) | tagggctagcCCGCCGGCGCTTGCGGGA | |
| O128 | O128 ZF21-16-F(G_mNc|e) | gcgccggcggGCTAGCCCTAGAGTGAGAACAGG | |
| O129 | O129 ZF21-16-R(G_SV40NLS) | tacctcctcctccGGATCCCTTCTCGCCTGTG | |
| O130 | O130 SV40NLS-F(G_ZF21-16) | gaagggatccGGAGGAGGAGGTAGTCCAAAAAAG | |
| O131 | O131 mNc|e-R(G_ZF42-10) | tagggctagcCCGCCGGCGCTTGCGGGA | |
| O132 | O132 ZF42-10-F(G_mNc|e) | gcgccggcggGCTAGCCCTAGAGTGAGAACAG | |
| O133 | O133 ZF42-10-R(G_SV40NLS) | tacctcctcctccGGATCCCTTTTCTCCGGTG | |
| O134 | O134 SV40NLS-F(G_ZF42-10) | aaagggatccGGAGGAGGAGGTAGTCCAAAAAAG | |
| O135 | O135 mNc|e-R(G_ZF43-8) | tggggctagcCCGCCGGCGCTTGCGGGA | |
| O136 | O136 ZF43-8-F(G_mNc|e) | gcgccggcggGCTAGCCCCAGAGTGAGAAC | |
| O137 | O137 ZF43-8-R(G_SV40NLS) | tacctcctcctccGGATCCTTTTTCACCTGTATGTG | |
| O138 | O138 SV40NLS-F(G_ZF43-8) | aaaaggatccGGAGGAGGAGGTAGTCCAAAAAAG | |
| O139 | O139 mNc|e-R(G_ZF54-8) | tgggagaagcCCGCCGGCGCTTGCGGGA | |
| O140 | O140 ZF54-8-F(G_mNc|e) | gcgccggcggGCTTCTCCCAGAGTAAGAACGGG | |
| O141 | O141 ZF54-8-R(G_SV40NLS) | tacctcctcctccGGACCCTTTTTCGCCGGTG | |
| O142 | O142 SV40NLS-F(G_ZF54-8) | aaaagggtccGGAGGAGGAGGTAGTCCAAAAAAG | |
| O143 | O143 mNc|e-R(G_ZFHD1) | atgggcgttcCCGCCGGCGCTTGCGGGA | |
| O144 | O144 ZFHD1-F(G_mNc|e) | gcgccggcggGAACGCCCATATGCTTGC | |
| O145 | O145 ZFHD1-R(G_SV40NLS) | tacctcctcctccGTTGATTCTTTTTTCTTTCTGGC | |
| O146 | O146 SV40NLS-F(G_ZFHD1) | aagaatcaacGGAGGAGGAGGTAGTCCAAAAAAG | |
| O147 | O147 EcoRV-mcherry-F(KOD) | GATATCATGGTGAGCAAGGGCGAGGAGG | |
| O148 | O148 P2A-F(6-AgeI-EcoRI) | GCAGCAACCGGTGGTGGAGAATTCggAtcAggTgccaccaacttctccctgctgaag | |
| O149 | O149 EGFP-R(HindIII-6) | GCAGCAAAGCTTTTActtgtacagctcgtccatgccga | |
| O150 | O150 KRAB-R(G_P2A) | AGTTGGTGGCACCTGATCCGAATTCCACCAGCCAGGGTTCCTCGC | |
| O151 | O151 KRAB-F(GGGGS-SV40NLS) | GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAatggacgccaagagcctg | |
| O160 | lacIF | accccggccccagatccgatATGAAACCAGTAACGTTATAC | |
| O161 | LacIR | ggtaccgtcgAACCTTCCTCTTCTTCTTAG | |
| O162 | SV40F | gaggaaggttCGACGGTACCGCGGGCCC | |
| O163 | sv40R | attaataactaCACCATGGTGGCGACCGGTG | |
| O164 | pCAGF | caccatggtgTAGTTATTAATAGTAATCAATTACGGG | |
| O165 | pCAGR | tcgcccttgctcaccatgatCTGTAGGAAAAAGAAGAAGG | |
| O166 | O166 tTa-R(EcoRI-6) | GCAGCAGAATTCCTACCCACCGTACTCGTCAATTCC | |
| O167 | O167 tTa-R(EcoRI-6 | f) | GCAGCAGAATTCCCCACCGTACTCGTCAATTCC |
| O168 | O168 EGFP-R(S) | gctgaacttgtggccgtttac | |
| O169 | O169 mcherry-R(S) | TGATGATGGCCATGTTATCCTCC | |
| O170 | O170 PIR/ZFD F | tttctggaattcgcggccgcttcTAGag | |
| O171 | 0171 pSBIC3 F | Ggcagaatttcagataaaaaaaatccttagctttcgctaaggatg | |
| O172 | 0172 pSBIC3 R | gcagggtggtgacaccttgcccttttttg | |
| O173 | O173NPminCMV | GAATTCGCGGCCGCTTCTAgagaggtaggcgtgtacggtg | |
| O174 | O174 P2A-R(G_EcoRV-mcherry) | TCGGATCTGGGGCCGGGGTTCTCCTCCACGTCGC | |
| O175 | O175 mcherry-F(G_P2A-EcoRV) | ccggccccAGATCCGATATCATGGTGAGCAAG | |
| O176 | O176 SV40poly(A)-F | AACTTGTTTATTGCAGCTTATAATGG | |
| O177 | O177 SV40poly(A)-R1 | TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTG | |
| O178 | O178 SV40poly(A)-R2 | GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACC | |
| O179 | O179 rtTA-F-(AgeI) | tttctgACCGGTATGTCTAGATTAGATAAAAGTAAAGTGATTAACAGCG | |
| O180 | O180 P2A-R(G2_mcherry) | ccatggtggcgatatcGGGGCCGGGGTTCTCCTC | |
| O181 | O181 mcherry-F(G2_P2A) | ccggccccgatatcgccaccATGGTGAGCAAGGGCGAGGagg | |
| O182 | O182 Kaba_B_R | taaacaagttctaCACCAGCCAGGGTTCCTC | |
| O183 | 0183 LPA_F | gctggtgtagAACTTGTTTATTGCAGCTTATAATG | |
| O184 | O184 LPA_R | catccttagcGATCCAGACATGATAAGATAC | |
| O185 | O185Prom_F | tgtctggatcGCTAAGGATGATTTCTGGAATTCGCGGC | |
| O186 | O186Prom_R | ttgctcaccatggtggcgatTTGCCGGACTGCAGCGGC | |
| O187 | O187 TF1_F(same with tf4) | aggagaaccccggccccgatGCTAGCCCTAGAGTGAGAAC | |
| O188 | O188TF2_F | aggagaaccccggccccgatGCTAGCCCCAGAGTGAGAAC | |
| O189 | O189TF3_F | aggagaaccccggccccgatGCTTCTCCCAGAGTAAGAACG | |
| O190 | O190TF8_F | aggagaaccccggccccgatGAACGCCCATATGCTTGC | |
| O191 | O191TF9_F | aggagaaccccggccccgatATGTCAAGGGGGGAGGTTAG | |
| O192 | O192lacI_F | aggagaaccccggccccgatATGAAACCAGTAACGTTATAC | |
| O193 | O193LacI_R | taaacaagttctaAACCTTCCTCTTCTTCTTAG | |
| O194 | O194lacI_40_F | gaggaaggtttagAACTTGTTTATTGCAGCTTATAATG | |
| O195 | O195LacI_40_R | attaataactaGATCCAGACATGATAAGATAC | |
| O196 | O196pCAGop_F | tgtctggatcTAGTTATTAATAGTAATCAATTACGGG | |
| O197 | O197pCAGop_R | ttgctcaccatggtggcgatCTGTAGGAAAAAGAAGAAGG | |
| O198 | O198 VP64-F(GGGGS-SV40NLS) | GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAGACGCGCTGGACGatt | |
| O199 | O199 VP64-R | TAACATATCGAGATCGAAATCGTCCAGag | |
| O200 | O200 VP64-F2(GSSSS-SV40NLS) | GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAgatgctttagacgattttgactt | |
| O201 | O201 VP64-R2 | tagcatatctagatcaaagtcatctagg | |
| O202 | O202 rtTAA-F(6-AgeI) | GCAGCAACCGGTatgtctagactggacaagagcaaag | |
| O203 | O203 rtTAA-R(HindIII-6) | TGCTGCAAGCTTttacccggggagcatgtcaag | |
| O204 | O204 gal_F | aggagaaccccggccccgatATGAAGCTGCTGAGCAGC | |
| O205 | O205_Gal_poly(a)_R | acatgagatcGATCCAGACATGATAAGATAC | |
| O206 | O206_Uas_F | tgtctggatcGATCTCATGTGATTACGCC | |
| O207 | O207_Uas_R | ttgctcaccatggtggcgatTGTACAAACTTGGGTCGAATTG | |
| O208 | O208 PGK-F(6-EcoRI-6-BamHI-6-MfeI) | GCAGCAGAATTCGGTGGAGGATCCGGAGGTCAATTGgggtaggggaggcgc | |
| O209 | O209 PuroR-R(NotI-6) | TGCTGCGCGGCCGCtcaggcaccgggcttg | |
| O210 | O210 EGFP-R(NotI-6-HindIII6) | TGCTGCAAGCTTTGCAGCGCGGCCGCTTActtgtacagctcgtccatgccgag | |
| O211 | O211 rtTAA-F(6-EcoRI) | GCAGCAGAATTCatgtctagactggacaagagcaaag | |
| O212 | O212 rtTAA-R(BamHI-6) | TGCAGCGGATCCttacccggggagcatgtcaag | |
| O213 | O213 Gal4DBD-F | ATGAAGCTGCTGAGCAGCatc | |
| O214 | O214 PIP-F | ATGTCAAGGGGGGAGGTTAG | |
| O215 | O215 ZF21-16|24-10-F | GCTAGCCCTAGAGTGAGAACAGG | |
| O216 | O216 ZF43-8-F | GCTAGCCCCAGAGTGAGAAC | |
| O217 | O217 ZF54-8 | GCTTCTCCCAGAGTAAGAACGG | |
| O218 | O218 ZFHD1-F | GAACGCCCATATGCTTGCC | |
| O219 | O219 M13fwdL | gtgtaaaacgacggccagtg | |
| O220 | O220 M13revL | gctatggacaggaaacagctatgac | |
| O221 | O221 G_puro_F | tgacatgctccccgggtaaaGCAGCAGAATTCGGTGGAGGATC | |
| O222 | O222G_puro_R | aagttatattaagggttccaTCAGGCACCGGGCTTGCG | |
| O223 | O223 G_pCAGop_R | ttgctcaccatggtggcgatATGAGACAGCACAACAAC | |
| O224 | O224 PGK-R(S) | gtgtagcgccaagtgcc | |
| O225 | O225 Ne-R(KOD) | CCCTGATTGTAGCAGGGGTTGCTAC | |
| O226 | O226 Ne-F(KOD) | CACCTGTGAGCCCACATCCGAGAAC | |
| O227 | O227 M13revL2 | AATTTCACACAGGAAACAGCTATGAC | |
| O228 | O228 EGFP-F(6-MfeI-Kozak) | GCAGCACAATTGGCCACCatggtgagcaagggcgag | |
| O229 | O229 mCherry-F(6-Kozak-MfeI) | GCAGCAICAATTGGCCACCATGGTGAGCAAGGGCGAG | |
| O230 | O230 PGK-F(6-EcoRI-6-BamHI-6-SalI) | GGAGGTGAATTCGGTGGAGGATCCGGAGGTGTCGACgggtaggggaggcgc | |
| O231 | O231 PGK-R(MfeI-6) | GCAGCACAATTGcgaaaggcccggagatgag | |
| O232 | O232 mCherry-R(NotI-6-HindIII-6) | GGTGGTAAGCTTGGTGGAGCGGCCGCTTACTTGTACAGCTCGTCCATGCC | |
| O233 | O233 Gal4DBD-F(6-MfeI-Kozak) | GCAGCACAATTGGCCACCATGAAGCTGCTGAGCAGCatc | |
| O234 | O234 PIP-F(6-MfeI-Kozak) | GCAGCACAATTGGCCACCATGTCAAGGGGGGAGGTTAG | |
| O235 | O235 ZF21-16|42-10-F(6-MfeI-Kozak) | GCAGCACAATTGGCCACCatgGCAAGCCCTAGAGTGAGAACAGgttc | |
| O236 | O236 ZF43-8-F(6-MfeI-Kozak) | GCAGCACAATTGGCCACCatgGCAAGCCCCAGAGTGAGAACTGGTTCTAAGACACCTCC | |
| O237 | O237 ZF54-8-F(6-MfeI-Kozak) | GCAGCACAATTGGCCACCatgGCTTCTCCCAGAGTAAGAACGG | |
| O238 | O238 ZFHD1-F(6-MfeI-Kozak) | GCAGCACAATTGGCCACCATGGAACGCCCATATGCTTGCc | |
| O239 | O239 KRAB-R(TAA-HindIII-6) | GCAGCAAAGCTTTTACACCAGCCAGGGTTCCTC | |
| O240 | O240 VP64-R(TAA-HindIII-6) | GCAGCAAAGCTTTTAtagcatatctagatcaaagtcatctagggcatc | |
| O241 | O241 P2A-R(MfeI-6-HindIII-6) | TCCTCCAAGCTTTCCTTACAATTGGGGGCCGGGGTTCTC | |
| O242 | O242 EGFP|mCherry-F(6-AgeI-6-EcoRI-Kozak) | GGAGGAACCGGTGGAGGTGAATTCGCCACCATGGTGAGCAAGGGCGAGGAG | |
| O243 | O243 EGFP|mCherry-F(6-BamHI-AgeI) | GCAGCAGGATCCACCGGTgccaccatggtgagcaagggcgag | |
| O244 | L.Lanier loxp seq | tggtgggtacctagtggaacc | |
| O245 | O245 pSB1C3-BS(L)(AgeI-6) | GCAGCAACCGGTgccggactgcagcggccgc | |
| O246 | O246 EGFP-F(L_CD8-alpha-SP1) | tgctgctgcccctggccctgctgctgcacgccgccagaccTatggtgagcaagggcgag | |
| O247 | O247 EGFP-F(L_CD8-alpha-SP2) | ATGgccctgcccgtgaccgccctgctgctgcccctgg | |
| O248 | O248 CD8-alpha-SP-F(6-AgeI) | GCAGCAACCGGTGCCACCATGgccctgcccgt | |
| O249 | O249 EGFP-R(OE_CD86tm) | gtgaagataaaagccgcttgtacagctcgtccatgc | |
| O250 | O250 CD86tm-F(OE-EGFP) | ggacgagctgtacaagcggcttttatcttcacctttctct | |
| O251 | O251 CD86-R(HindIII-6) | GCAGCAAAGCTTTCAatcccgaggccgcttc | |
| O252 | O252 CD86tm-F(L) | cggcttttatcttcacctttctctatagagcttgaggaccctcagc | |
| O253 | O253 uas-t-R | gagatgatccagacatgataagatacattg | |
| O254 | O254 uas-t-F | cttctggatccaattcgacccaag | |
| O255 | O255 Sv40-UAS-F | tatcatgtctggatcatctcGTGTGTCAGTTAGGGTGTG | |
| O256 | O256 SV40-UAS-R | tggcgtaatcTTTGCAAAAGCCTAGGCC | |
| O257 | O257 Gal4BDF | cttttgcaaaGATTACGCCAAGCTACGG | |
| O258 | O258 Gal4BDR | ggtcgaattggatccagaagTATCTAGAGTCTCCGCTC | |
| O259 | O259 mCherry-F(6-Kozak-MfeI) | GCAGCACAATTGGCCACCATGGTGAGCAAGGGCGAG | |
| O260 | O260 UAS-pre | ttctggAATTCGCGGCCGCTTCTAGaggatcatctcgtgtgtcagtta | |
| O261 | O261 UAS-suf | gccggacTGCAGCGGCCGCTACTAGTAagaaggtaactagagtctccgctcg | |
| O262 | O262 LaG16-F(L_CD8-alpha-SP1) | CGCCAGACCTGAGCAAAAGTTGATCTCAGAGGAGGACCTCATGACCATGGCCCAGGTG | |
| O263 | O263 P2A-R(L_CD8-alpha-SP1) | GCAGGGCCAGGGGCAGCAGCAGGGCGGTCACGGGCAGGGCCATGGGGCCGGGGTTCTC | |
| O264 | O264 P2A-R(L_CD8-alpha-SP2) | CTCCTCTGAGATCAACTTTTGCTCAGGTCTGGCGGCGTGCAGCAGCAGGGCCAGGGGC | |
| O265 | O265 P2A-R(L_CD8-alpha-SP2-HA) | GTAATCTGGCACGTCATAGGGGTAAGGTCTGGCGGCGTGCAGCAGCAGGGCCAGGGGC | |
| O266 | O266 alpha-GPC3-F(L_CD8-alpha-SP1) | CTTACCCCTATGACGTGCCAGATTACGCGGATGTTGTGATGACTCAGTCTCCACTCTC | |
| O267 | O267 LaG16-F(L_CD8-alpha-SP2) | GACCGCCCTGCTGCTGCCCCTGGCCCTGCTGCTGCACGCCGCCAGACCTGAGCAAAAG | |
| O268 | O268 CD8-alpha-SP-F(6-AgeI-Kozak) | GCAGCAACCGGTGCCACCATGGCCCTGCCCGTGACCGCCCTGCTGCTGCCCC | |
| O269 | O269 VP64-R(EcoRI-6 | f) | GCAGCAGAATTCtagcatatccagatcaaagtcatctagggcatcgg |
| O270 | O270 pUAS-F(6-BP) | GCAGCAGAATTCGCGGCCGCTTCTAGAGcatgtgattacgccaagctacggg | |
| O271 | O271 pUAS-R(BS-6) | GCAGCACTGCAGCGGCCGCTACTAGTActagagtctccgctcggaggacaga | |
| O272 | O272 P2A-F(S_6-BP) | GCAGCAGAATTCGCGGCCGCTTCTAGAGACCAACTTCTCCCTGCTGAAG | |
| O273 | O273 pML2-R(S_BS-6) | GCAGCACTGCAGCGGCCGCTACTAGTAacgagattccagcaggtcg | |
| O274 | O274 pCMV-F(S_6-BP) | GCAGCAGAATTCGCGGCCGCTTCTAGAGcgtgtacggtgggaggtctatataag | |
| O275 | O275 mTagBFP2-F(4-EcoRV-Kozak) | CGGccccGATATCGCCAccATGGTGTCTAAGGGCGAAGAGCTGATTAAGGAGAACATGC | |
| O276 | O276 mTagBFP2-F(L1-T2A) | GGGCAGGGGAAGTCTTCTAACtTGCGGGGACGTGGAGGAAAATCCCGGCCCCGATATCG | |
| O277 | O277 mTagBFP2-F(L2-T2A) | GCAGCAaCCGGTGGTGAATTCGGACAATTGggAtcAggTGAGGGCAGGGGAAGTC | |
| O278 | O278 mTagBFP2-R(HindIII-6) | GCAGCAAAGCTTTTAATTAAGtTTGTGCCCCAGTTTGCTAGG | |
| O279 | O279 EBFP2-F(4-EcoRV-Kozak) | CGGCCCCGATATCGCCAccatggtgagcaagggcg | |
| O280 | O280 EBFP2-R(HindIII-6) | GCAGCAAAGCTTttacttgtacagctcgtccatg | |
| O281 | O281 mTagBFP2-F(L2-T2A) | GCAGCAGAATTCGGTaCCGGTGGACAATTGggAtcAggTGAGGGCAGGGGAAGTC | |
| O282 | O282 PIP-F(6-AgeI) | GCAGCAACCGGTGCCACCATGTCAAGGGGGGAGGTTAG | |
| O283 | O283 ZF43-8-F(6-AgeI) | GCAGCAACCGGTGCAAGCCCCAGAGTGAGAAC | |
| O284 | O284 PIP-F(6-AgeI-Kozak) | GCAGCAACCGGTGCCACCatggtgTCAAGGGGGGAGGTTAGAATGG | |
| O285 | O285 ZF43-8-F(6-AgeI-Kozak) | GCAGCAACCGGTGCCACCatgGCAAGCCCCAGAGTG | |
| O286 | O286 P2A-F(6-SbfI-C) | GCAGCACCTGCAGGCggctccggcgccac | |
| O287 | O287 KRAB-R(MfeI-6) | GCAGCACAATTGcaccagccagggttcctc | |
| O288 | O288 mTagBFP2-NR(S) | GCACTTGAAGTGATGGTTGTCC | |
| O289 | O289 P2A-F(3-AgeI-Kozak-ATG-EcoRI-BamHI-SalI) | GCAACCGGTGAATTCGCCACCATGGGATCCGTCGACGGATCAGGTGCCACCAAC | |
| O290 | O290 mCherry-R(CAAX-HindIII-1) | TAAGCTTtcaggagagcacacacttgcagctcatgcagccCTTGTACAGCTCGTCCATG | |
| O291 | O291 mCherry-F(LifeAct-L1) | AGTCCATCTCCAAGGAGGAGGGCGATCCCCCGGTCGCCACCATGGTGAGCAAGGGCGAG | |
| O292 | O292 mCherry-F(LifeAct-L2) | ATGGGCGTGGCCGACTTGATCAAGAAGTTCGAGTCCATCTCCAAGGAGGAGG | |
| O293 | O293 LifeAct-F(3-EcoRI-BamHI-Kozak) | GCAGAATTCGGATCCGCCACCATGGGCGTGGCCGAC | |
| O294 | O294 mCherry-R(P2A-L1) | CGCcggcctgcttcagcagggagaagttggtggcgccggagccCTTGTACAGCTCGTCC | |
| O295 | O295 mCherry-R(P2A-L2) | TAAGCTTGTCGACCTACAATTGGGGGCCGGGGTTCTCctccacgtcgccggcctgcttc | |
| O296 | O296 Hist2h2bb-F(3-EcoRI-BamHI-Kozak) | GGTGAATTCGGATCCGCCACCatgccggacccagcgaag | |
| O297 | O297 Hist2h2bb-R(M) | cgttcacgaaggaGttcatgattcccatagccttagatgagatg | |
| O298 | O298 Hist2h2bb-F(M) | catgaaCtccttcgtgaacgaCatcttcgagcgcatcgc | |
| O299 | O299 Hist2h2bb-R(OE_mCherry) | CATTgtGgcCacAggAggGtccttggagctggtgtacttggtg | |
| O300 | O300 mCherry-F(OE_Hist2h2bb) | acccTccTgtGgcCacAATGGTGAGCAAGGGCGAG | |
| O301 | O301 mCherry-R(OE_mito) | cgcctctcccgttCTTGTACAGCTCGTCCATGCC | |
| O302 | O302 mito-F(OE_mCherry) | GTACAAGaacgggagaggcggtagac | |
| O303 | O303 mito-R(HindIII-3) | GCAAAGCTTttaattattttttcttaattgaataattttgataaacgcccc | |
| O304 | O304 Myc-F | GAGCAGAAATTGATCTCTGAAGAGGAC | |
| O305 | O305 pML2-C0-R | gcatacattatacgaagttatattaagggttccaag | |
| O306 | O306 mNce-F | CCCTGCGTCGGTAGCAAC | |
| O307 | O307 tTAA-F(3-MfeI) | GCACAATTGATGAGTCGATTGGATAAATCTAAGGTAATTAACAG | |
| O308 | O308 tTAA-R(HindIII-3) | GCAAAGCTTTCATCCTGGGAGCATATCCAGG | |
| O309 | O309 TetOn3G-F(3-MfeI) | GCACAATTGATGAGCCGACTGGACAAGAG | |
| O310 | O310 TetOn3G-R(HindIII-R) | GCAAAGCTTTTAGCCCGGCAACATATCAAGATC | |
| O311 | O311 pSV40-F(3-BP) | GCAGAATTCGCGGCCGCTTCTAGAGgtgtgtcagttagggtgtgg | |
| O312 | O312 pSV40-R(BS-AgeI-3) | GCAACCGGTctgcagcggccgcTACTAGTAtttgcaaaagcctaggcctcc | |
| O313 | O313 pTight-F(3-BP) | GCAGAATTCGCGGCCGCTTCTAGAGGCCGGAATTGCTCGAGTTTAC | |
| O314 | O314 pTight-R(BS-AgeI-3) | GCAACCGGTctgcagcggccgctactagtaGGCGATCTGACGGTTCACTAAAC | |
| O315 | O315 TRE3GV-F(3-BP) | GCAgaattcgcggccgcttcTAGAGCCCTTTCGTCTTCACTCGAGTTTAC | |
| O316 | O316 TRE3GV-R(BS-AgeI-2) | CAACCGGTctgcagcggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc | |
| O317 | O317 minCMV-F(3-BP) | GCAgAATTCGCGGCCGCTTCTAGAGGGTAGGCGTGTACGGTGG | |
| O318 | O318 minCMV2-F(3-BP) | GCAgaattcgcggccgcttcTAGAGGGTAGGCGTGTACGGTGG | |
| O319 | pSB1C3-VF(S) | TGCCACCTGACGTCTAAGAA | |
| O320 | pSB1C3-VR(S) | ATTACCGCCTTTGAGTGAGC | |
| O321 | prefix-mNotch(e)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGCCCTGCGTCGGTAGCA | |
| O322 | mNotch(e)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTACCGTCGGCGCTTTCG | |
| O323 | prefix-hNotch(e)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGCCCTGTCTTGGAGGGAATCC | |
| O324 | hNotch(e)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTACCGGCGTCGTTTTCGAC | |
| O325 | pSB1C3-F | GCAGCATACTAGTAGCGGCCGCTG | |
| O326 | pSB1C3-R | GCAGCACTCTAGAAGCGGCCGCG | |
| O327 | prefix-LaG17(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGTGAACCGTCAGATCAGATCCGCTAG | |
| O328 | LaG17(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGCA | |
| O329 | prefix-CAR3G(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGCCAGCCAAACCAACAACTACAC | |
| O330 | CAR3G(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccAAGCT | |
| O331 | prefix-EGFPtm(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGTGGAAGAAAATCCCGGTCCG | |
| O332 | EGFPtm(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagct | |
| O333 | prefix-tTAA(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGTGTCACGAAAGCGCCG | |
| O334 | tTAA(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagct | |
| O335 | IL-2(CO)-F(G_pSB1C3) | GAAGATCTCACGACGtgaaccgtcagatccgctag | |
| O336 | IL-2(CO)-R(G_pSB1C3) | GATGATCATACGACGATACGAAGTTATATTAAGGGTTCCAAGC | |
| O337 | IL-10(CO)-F(G_pSB1C3) | GAAGATCTCACGACGtgaaccgtcagatccgctag | |
| O338 | IL-10(CO)-R(G_pSB1C3) | GATGATCATACGACGATACGAAGTTATATTAAGGGTTCCAAGC | |
| O339 | LaG16-2(CO)-F(G_pSB1C3) | GAAGATCTCACGACGtgaaccgtcagatccgctag | |
| O340 | LaG16-2(CO)-R(G_pSB1C3) | GATGATCATACGACGGGGTTGCTACCGACGC | |
| O341 | prefix-CD47tm(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc | |
| O342 | CD47tm(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTACGGACCGGGATTTTCTTCC | |
| O343 | prefix-hYP9 | 1scFv(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc |
| O344 | hYP9 | 1scFv(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAACCTGGGACCCCCC |
| O345 | prefix-LaG 16-2(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC | |
| O346 | LaG 16-2(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGC | |
| O347 | prefix-OKT3scFv(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGGGTGGCGGGGGGTC | |
| O348 | OKT3scFv(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagcTTC | |
| O349 | prefix-Tet-On3G(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGGGAGGTgaattcGCCACC | |
| O350 | Tet-On3G(CO)-R -suffix | GCAGCACTGCAGCGGCCGCTACTAGTAACCTCCggatccTTAGCC | |
| O351 | prefix-α-CD19(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC | |
| O352 | α-CD19(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGCAG | |
| O353 | prefix-α-CD47(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC | |
| O354 | α-CD47(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAGTAGTTGTTGGTTTGGCTGGC | |
| O355 | prefix-IL-2(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc | |
| O356 | IL-2(CO)-R-suffix | GCAGCACTGCAGCGGCCGCTACTAGTAATACGAAGTTATATTAAGGGTTCCAAGC | |
| O357 | IL-10(CO)-F | GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC | |
| O358 | IL-10(CO)-R | GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagctTC | |
| O359 | O359 pML2-C0(AgeI) | tgaaccgtcagatccgctagc | |
| O360 | O360 pML2-C0(HindIII) | atacgaagttatattaagggttccaagc | |
| O361 | O361 mNc-F | ATACTCGACTACAGTTTTACTGGGG | |
| O362 | O362 LaG2-R(OE_mNc) | CCCCAGTAAAACTGTAGTCGAGTATAACAGTGACTTGCGTTCCCTG | |
| O363 | O363 LaG17-R(OE_mNc) | CCCCAGTAAAACTGTAGTCGAGTATGGAAACGGTCACCTGTGTACC | |
| O364 | O364 CD8SP-F(3-AgeI-Kozak) | GCAACCGGTGCCACCATGGCCCTTCCTGTAACGG | |
| O365 | O365 PDGFRB-R(TGA-HindIII-3) | GCAAAGCTTTCATCTAGGTTTCTTCTGCCAAAGCATGATC | |
| O382 | O382 2*ZF21-16-F(Scar) | tactagattagaagtgaattagaagtga | |
| O383 | O383 2*ZF21-16-R(Scar) | ctagtatcacttctaattcacttctaat | |
| O384 | O384 BP-BbsI-F(3) | ctggaattcgcggccgcttctagaGAAGAC | |
| O385 | O385 3-BP-BbsI-R(Scar) | ctagtaGTCTTCtctagaagcggccgcgaattccag | |
| O386 | O386 BS-3-F | TACTAGTAGCGGCCGCTGCAGtcc | |
| O387 | O387 BS-R(3) | ggaCTGCAGCGGCCGCTA | |
| O388 | O388 EGFP-F(myr-L1) | ccaaggatgccagccagcggcgccgcGGTGGTGGAGGCTCAATGGTGAGCAAGGGCGAG | |
| O389 | O389 EGFP-F(myr-L2) | TGAATTCGGATCCGCCACCatgggtagcaacaagagcaagcccaaggatgccagccagc | |
| O390 | O390 2*ZF21-16-A-F(A) | tactagattagaagtgaattagaagtgatactag | |
| O391 | O391 2*ZF21-16-A-R(A) | tcacttctaattcacttctaat | |
| O392 | O392 2*ZF21-16-B-F(A) | attagaagtgaattagaagtga | |
| O393 | O393 2*ZF21-16-B-R(A) | ctagtatcacttctaattcacttctaatctagta | |
| O394 | O394 BP-F(A) | GATCCGaattcgcggccgcttctagaGAAGAC | |
| O395 | O395 BP-R(A) | ctagtaGTCTTCtctagaagcggccgcgaattcg | |
| O396 | O396 BS-F(A) | TAGCGGCCGCTGCAGA | |
| O397 | O397 BS-R(A) | AGCTTCTGCAGCGGCCGCTActagta | |
| O398 | O398 BP-F | Cgaattcgcggccgc | |
| O399 | O399 BS-R | TCTGCAGCGGCCGC | |
| O400 | O400 pSB1A3-C(3-BP-BamHI) | GCActctagaagcggccgcgaattcGGATCCgaaatcatccttagcgaaagctaagg | |
| O401 | O401 pSB1A3-C(3-BS-HindIII | C) | GCAtactagtagcggccgctgcagAAGCTTtccggcaaaaaagggcaagg |
| O404 | O404 mNCe-F(M) | CCTTGTGTTGGtAGTAATCCATGCTACAATCAAGGGACCTGCG | |
| O405 | O405 mNCe-F | CCTTGTGTTGGtAGTAATCCATGCTAC | |
| O406 | O406 mNC|e-R(M) | cctTCTtcttTTTCGTGACAGCAAAACACCGCAGCCTAC | |
| O407 | O407 mNC|e-R | cctTCTtcttTTTCGTGACAGCAAAAC | |
| O408 | O408 LaG2-R(G_mNCe) | ggattactaccaacacaaggAACAGTGACTTGCGTTCCCTGC | |
| O409 | O409 LaG17-R(G_mNCe) | ggattactaccaacacaaggGGAAACGGTCACCTGTGTACC | |
| O410 | O410 tTAA-F(G_mNCe) | TGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAG | |
| O411 | O411 pML2-CX-R(Lin) | CACCGGTaActcaagATgatctgacTAttcactacagctctgcttatatagacctccc | |
| O412 | O412 pML2-CX-F(Lin) | TCAATTGGGAACTCGATACCTTCTAATATCTCATAataacttcgtataatgtatgctatacgaagttattaggtccctcgacctgctg | |
| O413 | O413 MCS-F(A) | TGAATTCGGATCCAGGAGCAGTCTTCGGGCCCGAAGACTGCCATAGTCGACAAGCTTC | |
| O414 | O414 MCS-R(A) | GAAGCTTGTCGACTATGGCAGTCTTCGGGCCCGAAGACTGCTCCTGGATCCGAATTCA | |
| O415 | O415 pCMV-F2(S) | cccattgacgcaaatgggc | |
| O416 | O416 CD8aSP-F(Kozak | G_pML2-CX) | gtgaaTAgtcagatcATcttgagTtACCGGTGAATTCGCCACCATGGCGCTGCCGG |
| O417 | O417 LaG2-R(G_mNCe) | ATTaTAGCAaGGATTACTACCAACACAAGGAACAGTGACTTGCGTTCCCTGC | |
| O418 | O418 mNCe-F(M) | CCTTGTGTTGGTAGTAATCCtTGCTAtAATCAAGGGACCTGCGAGC | |
| O419 | O419 mNC-F(M) | ATACTCGACTAtAGTTTTACaGGGGGGGCGGGCCGGGATATACCCCCG | |
| O420 | O420 mNC|e-R(M) | CCTTCTTCTTTTTCGTGACAGCAAAACtCCGCAGCCTACGAAGAACAAGAGTAC | |
| O421 | O421 tTAA-F(M) | GGaGTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGG | |
| O422 | O422 tTAA-R(G_pML2-CX) | GAGATATTAGAAGGTATCGAGTTCCCAATTGAAGCTTTCATCCTGGGAGCATATCCAGG | |
| O423 | O423 pML2-R(Lin | L1) | tcaagATgatctgacTAttcactacagctctgcttatatagacctcccaccgtacacgc |
| O424 | O424 pML2-R(Lin | L2) | CGAATTCACCGGTaActcaagATgatctgacTAttcactacagctc |
| O425 | O425 pML2-F(Lin | L2) | CAAGCTTCAATTGGGAACTCGATACCTTCTAATATCTC |
| O426 | O426 pML2-CX(EcoRI) | gtagtgaaTAgtcagatcATcttgagTtACCGGTG | |
| O427 | O427 pML2-CX(HindIII) | TATGAGATATTAGAAGGTATCGAGTTCCCAATTGAAGC | |
| O428 | O428 pML2X-R(Lin1) | AAGACCACCGGTGAATTCattaagggttccggttccactaggtacccacc | |
| O429 | O429 pML2X-R(Lin2) | CGTCGACTAGCCCTAACGTGATCTGACAGTACATTAAGACCACCGGTGAATTCattaag | |
| O430 | O430 pML2X-F(Lin1) | TGAtaggtccctcgacctgctggaatctcgtg | |
| O431 | O431 pML2X-F(Lin2) | GAAGCTTCCCTCATTCAAGTACCAGTCTAGTCGAAGTGTGAtaggtccctcgacctgc | |
| O432 | O432 MCS-F(A) | AGTCGACGGATCCAGGAGCAGTCTTCCTGCAGGAAGACTGCCATACAATTGAAGCTTC | |
| O433 | O433 MCS-R(A) | GAAGCTTCAATTGTATGGCAGTCTTCCTGCAGGAAGACTGCTCCTGGATCCGTCGACT | |
| O434 | O434 pML2X-F(S) | atcctccctttatccagccc | |
| O435 | O435 pML2X-R(S) | atcgataagctcgcttcacg | |
| O436 | O436 pML2Y-F(Lin1) | CGGCCGCtaggtccctcgacctgctggaatctcgtg | |
| O437 | O437 pML2Y-F(Lin2) | GAAGCTTCCCTCATTCAAGTACCAGTCTAGTCGAAGTGTGAGCGGCCGCtaggtccctc | |
| O438 | O438 pML2Y(G_SalI) | GGTCTTAATGTACTGTCAGATCACGTTAGGGC | |
| O439 | O439 pML2Y(G_HindIII) | TCACACTTCGACTAGACTGGTACTTGAATGAGG | |
| O440 | O440 pML2X-R(Lin2) | CGTCGACTAGCCCTAACGTGATCTGACAGTACAaTAAGACCACCGGTGAATTCattaag | |
| O441 | O441 pML2Y(G_SalI) | GGTCTTAtTGTACTGTCAGATCACGTTAGGGC | |
| O442 | O442 CMV-F(3-EcoRI) | GCAGAATTCcgttacataacttacggtaaatggcccgcctggctg | |
| O443 | O443 pCMV-R(AgeI-3) | GCAACCGGTagctctgcttatatagacctcccaccgtacacgcc | |
| O444 | O444 PGK-F(3-EcoRI) | GCAGAATTCgggtaggggaggc | |
| O445 | O445 PGK-R(AgeI-G_pML2Y-SalI) | CGTCGACTAGCCCTAACGTGATCTGACAGTACAATAAGACCACCGGTcgaaaggcccgg | |
| O446 | O446 CD8aSP-F(G_pML2Y) | GGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTGCCG | |
| O447 | O447 P2A-R(G_CD8aSP) | tacaggaagggccatCGGACCGGGATTTTCTTCCACGTC | |
| O448 | O448 CD8aSP-F(G_P2A) | gaaaatcccggtccgATGGCCCTTCCTGTAACG | |
| O449 | O449 PDGFRBtr-R(G_pML2Y) | TCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTTCAGCGAGGTTTCTTTTGC | |
| O450 | O450 CD8aSP-F(G_pML2Y) | GGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCG | |
| O451 | O451 tTAA-R(G_pML2Y) | TCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTTCATCCTGGGAGCATATC | |
| O452 | O452 LaG17-R(G_mNCe) | attatagcaaggattactaccaacacaaggGGAAACGGTCACCTGTGTACCTTGTCC | |
| O453 | O453 aCD19-R(G_mNCe) | attatagcaaggattactaccaacacaaggTGATGAAACGGTTACGGATGTCCCTTGTC | |
| O454 | O454 LaG16-2-R(G_mNC) | CGCCCCCCCTGTAAAACTATAGTCGAGTATAACAGTGACTTGCGTTCCCTGCC | |
| O455 | O455 LaG17-R(G_mNC) | cgccccccctgtaaaactatagtcgagtatGGAAACGGTCACCTGTGTACCTTGTCC | |
| O456 | O456 aCD19-R(G_mNC) | CGCCCCCCCTGTAAAACTATAGTCGAGTATTGATGAAACGGTTACGGATGTCCCTTGTC | |
| O457 | O457 CD19ECD-R(G_Myc) | gatcaatttctgctcCTTCCATCCCCCCGTGCG | |
| O458 | O458 Myc-F(G_CD19ECD) | acggggggatggaagGAGCAGAAATTGATCTCTGAAGAG | |
| O459 | O459 PGK-F(S) | cggcattctgcacgcttc | |
| O460 | O460 LaG16-F(L1) | acgccgccaggccgGAGCAGAAGCTGATCTCAGAGGAGGACCTGATGGCCCAGGTGCAG | |
| O461 | O461 LaG16-F(L2) | ggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggc | |
| O462 | O462 LaG16-F(L3) | tgaaccgtcagatccgctagcgctaCCGGTatggccttaccagtgaccg | |
| O463 | O463 mNC|e-R(M) | CCTTCTTCTTTTTCGTGACAGCAAAACTCCACAGCCCACAAAGAACAGG | |
| O464 | O464 pML2Y-R(C) | ACCACCGGTGAATTCattaagggttccggttccactaggtacccaccaaattccggcgc | |
| O465 | O465 pML2Y-F(C) | TGAGCGGCCGCtaggtccctcgacctgctggaatctcgtgaagcgagcttatcg | |
| O466 | O466 XXX-F(1-AgeI-pML2Y-SalI) | CACCGGTGGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACccggggtggtgc | |
| O467 | O467 XXX-R(HindIII-pML2Y-NotI-1) | aGCGGCCGCTCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTcccggcggcg | |
| O468 | ZF 21-16 | GCAGAATTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaTTAGAAGTGaTACTAGTAGCGGCCGCTGCAG | |
| O469 | O469 EGFP-F(3-SalI-Kozak-ATG-BamHI) | CTAGTCGACGCCACCATGGGATCCGTGAGCAAGGGCGAGGAG | |
| O470 | O470 EGFP/mCherry-R(L1) | GCTTGCTTTAACAGAGAGAAGTTCGTGGCgccggagccCTTGTACAGCTCGTCCATGCC | |
| O471 | O471 EGFP-R(L2) | TCAATTGAGGTCCCGGGTTTTCTTCCACGTCTCCTGCTTGCTTTAACAGAGAGAAGTTC | |
| O472 | O472 P2A-R(MfeI-BbsI-TGA-SbfI-BbsI-HindIII-3) | GGAAGCTTAGTCTTCCTGCAGGTCATGGAAGACTCAATTGAGGTCCCGGGTTTTCTTCC | |
| O473 | O473 CD8aSP-F(3-SalI-Kozak) | GCAGTCGACGCCACCatggccttaccagtgaccg | |
| O474 | O474 TRE3GV-F(3-EcoRI) | GCAGAATTCGAGTTTACTCCCTATCagtgatagagaacgtatgaag | |
| O475 | O475 TRE3GV-R(AgeI-3) | GCAACCGGTAAGTATAAGACAAAAGTGTTGTGGAATTGcTCC | |
| O476 | O476 mCherry-R(KOD) | CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCAC | |
| O477 | O477 mCherry-F(KOD) | CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCC | |
| O478 | O478 BP-F(M_3-EcoRI) | GCAGAATTCcggccgcttcTAGAG | |
| O479 | O479 BS-R(M_1-PstI-AgeI-3) | GCAACCGGTCTGCAGacggccgcTACTAGT | |
| O480 | ZF 43-8 | GCAGAATTCGCGGCCGCTTCTAGaGAGTGAGGAcaGAGTGAGGAcTACTAGTAGCGGCCGCTGCAG | |
| O481 | O481 HIST2H2BE-F(CE_pML2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGcctgaaccggcaaaatcc | |
| O482 | O482 HIST2H2BE-R(CE_EGFP) | tccttggagctggtgtacttggtgac | |
| O483 | O483 EGFP-F(CE_HIST2H2BE) | aagtacaccagctccaaggaCccTccTgtGgcCacAATGGTGAGCAAGGGCGAG | |
| O484 | O484 P2A-R(CE_pML2Y) | GTACTTGAATGAGGGAAGCTTAGTCTTCCTGCAGGTC | |
| O485 | O485 LifeAct-F(3-SalI) | CTAGTCGACGCCACCATGGGCGTGGCCGAC | |
| O486 | O486 myr-F(3-SalI) | CTAGTCGACGCCACCATGggtagcaacaagagcaagcccaaggatgccagccagc | |
| O487 | O487 1-BP-21-16-43-8-SpePI-1-F(A) | CGAATTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTG | |
| O488 | O488 1-BP-21-16-43-8-SpeI-1-R(A) | CACTAGTAgTCCTCACTCttCACTTCTAAtCtCTAGAAGCGGCCGCGAATTCG | |
| O489 | O489 1-XbaI-21-16-43-8-SpeI-BS-1-F(A) | GTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTAGCGGCCGCTGCAGC | |
| O490 | O490 1-XbaI-21-16-43-8-SpeI-BS-1-R(A) | GCTGCAGCGGCCGCTACTAGTAgTCCTCACTCttCACTTCTAAtCtCTAGAC | |
| O491 | O491 p2Y(CE_EcoRI) | accggaacccttaatGAATTC | |
| O492 | O492 p2Y(CE_AgeI) | CAGTACAATAAGACCACCGGT | |
| O493 | O493 p2Y(CE_SalI) | ATCACGTTAGGGCTAGTCGAC | |
| O494 | O494 p2Y(CE_HindIII) | GTACTTGAATGAGGGAAGCTT | |
| O495 | O495 mNC-F(M) | ATTCTCGACTACAGTTTCACAGGAGGAGCGGGCCGGGATATACCCCCG | |
| O496 | O496 tTAA-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCATCCTGGGAGCATATCCAGGTC | |
| O497 | O497 CD8aSP-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGG | |
| O498 | O498 LaG2-R(CE_mNC) | GTGAAACTGTAGTCGAGAATAACAGTGACTTGCGTTCCC | |
| O499 | O499 LaG17-R(CE_mNC) | GTGAAACTGTAGTCGAGAATGGAAACGGTCACCTGTGTACC | |
| O500 | O500 aCD19-R(CD_mNC) | GTGAAACTGTAGTCGAGAATTGATGAAACGGTTACGGATGTCC | |
| O501 | O501 PGK-F(CE_p2Y) | accggaacccttaatGAATTCgggtaggggaggcgc | |
| O502 | O502 PGK-R(CE_p2Y) | CAGTACAATAAGACCACCGGTcgaaaggcccggagatgag | |
| O503 | O503 CD8aSP-F2(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTGCCGGTC | |
| O504 | O504 CD19ECD(CE_Myc) | atttctgctcCTTCCATCCCCCCGTGC | |
| O505 | O505 Myf-F(CE_CD19ECD) | gggatggaagGAGCAGAAATTGATCTCTGAAGAGGAC | |
| O506 | O506 PDGFRBtr-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCATCTAGGTTTCTTCTGCCAAAGCATG | |
| O507 | O507 TetOn3G-F(CE_P2A) | TGGAAGAAAACCCGGGACCTATGAGCCGACTGGACAAGAGC | |
| O508 | O508 TetOn3G-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTTACAATTGGCCCGGCAACATATCAAGATCAAAGTC | |
| O509 | O509 U105 pEGFP-C5 | CATGGTCCTGCTGGAGTTCGT | |
| O510 | O510 mCherry-R(KOD) | CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCTTGCAGGGAGGAGTCCTGGGTCAC | |
| O511 | O511 mCherry-F(KOD) | CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAAAAGACCATGGGCTGGGAGGCC | |
| O512 | O512 BP-F(M | CE_p2Y-EcoRI) | accggaacccttaatGAATTCcggccgcttcTAGAG |
| O513 | O513 BS(M | CE_p2Y-AgeI) | CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT |
| O514 | O514 BS-R(M | CE_p2Y-AS) | CCCTAACGTGATCTGACAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT |
| O515 | O515 PIP-F(CE_p2Y-AS-Kozak) | ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGTCAAGGGGGGAGGTTAG | |
| O516 | O516 KRAB-R(CE_HindIII) | GTACTTGAATGAGGGAAGCTTTTACACCAGCCAGGGTTCC | |
| O517 | O517 pSV40-F(CE_p2Y-EcoRI) | accggaacccttaatGAATTCcggccgcttcTAGAGgtgtgtcagttagggtgtgg | |
| O518 | O518 ZF21-16-F(CE_p2Y-EcoRI) | accggaacccttaatGAATTCcggccgcttcTAGAGaTTAGAAGTG | |
| O519 | O519 CE_P2A-R | AGGTCCCGGGTTTTCTTCC | |
| O520 | O520 TetOn3G-F(CE_P2A-MfeI) | TGGAAGAAAACCCGGGACCTCAATTGATGAGCCGACTGGACAAGAGC | |
| O521 | O521 TetOn3G-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTTAGCCCGGCAACATATCAAGATCAAAGTC | |
| O522 | O522 TRE3GV-F(1-BP-M_p2Y) | CGAATTCcggccgcttcTAGAGGAGTTTACTCCCTATCagtgatagagaacgtatgaag | |
| O523 | O523 TRE3GV-R(BS-M_p2Y-AgeI-1) | GACCGGTCTGCAGacggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc | |
| O524 | O524 ZF43-8-F(CE_p2Y | M) | accggaacccttaatGAATTCcggccgcttcTAGAGaGAGTGAGG |
| O525 | O525 LaG16-F(CE-P2Y_SalI) | ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGGTCaccgccttgctcctgc | |
| O526 | O526 ZF43-8-F(CE_P2A) | TGGAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC | |
| O527 | O527 ZF43-8-R | GGAGCCCTTCTCACCTGTATGTGTTTTCAGATGtctgttc | |
| O528 | O528 G4S-SV40NLS-F(CE_SynTF-C) | ATACAGGTGAGAAGGGCTCCGGAGGAGGAGGTAGTCCAAAAAAG | |
| O529 | O529 KRAB-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTCACAATTGCACCAGCCAAGGTTCTTCTCccttttcc | |
| O530 | O530 PIP-F(CE_P2A) | TGGAAGAAAACCCGGGACCTATGTCAAGGGGGGAGGTTAG | |
| O531 | O531 ZF43-8-F(CE_p2Y-SalI) | ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGAG | |
| O532 | O532 HA-F(CE_pGEX) | TTCCAGGGGCCCCTGGGATCCTACCCCTATGATGTACCAGATTACGC | |
| O533 | O533 Myc-R(CE_pGEX) | CTCGAGTCGACCCGGGAATTCTCACAGGTCCTCTTCAGAGATCAATTTC | |
| O534 | O534 TetOn3G-F(CE_P2A-MfeI) | GAAAACCCGGGACCTCAATTGATGAGCCGACTGGACAAGAGCAAGGTAATC | |
| O535 | O535 TetOn3G-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTTAGCCCGGCAACATATCAAGATCAAAGTCGTCG | |
| O536 | O536 CD8aSP-F(CE_P2A) | GTCCGATGGCCCTTCCTGTAACGG | |
| O537 | O537 PDGFRBtr(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTCAGCGAGGTTTCTTTTGCCAGAG | |
| O538 | O538 T2A-F(CE_p2Y-SalI-BamHI) | ATCACGTTAGGGCTAGTCGACGCAGGATCCggAtcAggTGAAGGTAGGGGGTCATTGC | |
| O539 | O539 hYP9.1bscFv-R(CE_OKT3scFV) | GACTGTTGGAGTTGAACCTGACTACCTCCTCCACCCTTGATCTCAAGTTTGGTTCCTTG | |
| O540 | O540 OKT3scFv-F | CAGGTTCAACTCCAACAGTCTGG | |
| O541 | O541 OKT3scFv-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTCTAGCGGTTTATTTCAAGTTTTGTGCC | |
| O542 | O542 2.3D11scFv-R(CE_CAR3G) | GTAGTTGTTGGTTTGGCTGGCTTTATTTCGACTTTCGTGCCTCC | |
| O543 | O543 CAR3G-F | CCAGCCAAACCAACAACTACAC | |
| O544 | O544 CAR3G-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTCACCGTGGTGGCAAAGCC | |
| O545 | O545 IL-10-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTCAGTTCCTTATTTTCATCGTCATATACGCTTC | |
| O546 | O546 LaG17-R(CE_hNC) | GAAACTGTAGTCGAGAATGGAAACGGTCACCTGTGTACCTTGTCCC | |
| O547 | O547 hNC-F(M) | ATTCTCGACTACAGTTTCGGTGGGGGAGCGGG | |
| O548 | O548 hNC|e-R(M) | CCTTCTTCTTTTTCGTGACAGCAAAACTCCACACCCTACG | |
| O549 | O549 mTagBFP2-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGGTGTCTAAGGGCGAAGAG | |
| O550 | O550 mTagBFP2-R(M1) | CGTCCTCGTATGTGGTGACTCTCTCCCATGTGAAGCCC | |
| O551 | O551 mTagBFP2-F(M2) | AGTCACCACATACGAGGACGGGGGCGTGCTGACCG | |
| O552 | O552 mTagBFP2-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCAATTCAGCTTGTGCCCCAGTTTG | |
| O553 | O553 mNc F(CE/G_LNR-A [-]) | ATTCTCGACTACAGTTTCACAGGAGGAGCGTCACTTAACTTCAATGATCCGTGGAAG | |
| O554 | O554 mNc F(CE/G_LNR-ABLnkr[-]) | ATTCTCGACTACAGTTTCACAGGAGGAGCGTGCACCCAGTCCCTCCA | |
| O555 | O555 mNc F(CE_LNR-A[-]hlx3+) | ATTCTCGACTACAGTTTCACAATCACGCCTGCGGTT | |
| O556 | O539 mNC-R(M_S2) | GTTCGCTTTTgtcctCTTCGATTTTATACGGAATATTCAAGCTCCCAAGAGAGGCC | |
| O557 | O540 mNC-F(M_S2) | CGAAGaggacAAAAGCGAACCTGTCGAACCGCC | |
| O558 | O541 mNC-R(M_S3) | GCAAAAagaagagcagTACGAAGAACAAGAGTACGAACGCAGCAGC | |
| O559 | O542 mNC-F(M_S3) | CGTActgctcttctTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGG | |
| O560 | O543 MycoP-F | CACCATCTGTCACTCTGTTAA | |
| O561 | O544 MycoP-R | GGAGCAAACAGGATTAGATAC | |
| O562 | O562 BP-ZF21-16-43-8-BS | TTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTAGCGGCCGCTG | |
| O563 | O563 CE ZF21-16-43-8 F | TAAGGATGATTTCTGGAATTCGCGGCCGCTTc | |
| O564 | O564 CE ZF21-16-43-8 R | ttgcccttttttgccggaCTGCAGCGGCCGctac | |
| O565 | mNC-R-N(S) | CCTCTGTCAGTTGGCAGTC | |
| O566 | O566 pGEX usual F(S) | GAGCTGCATGTGTCAGAGG | |
| O567 | O567 pGEX usual R(S) | GCAAGCCACGTTTGGTG | |
| O568 | O568 d2EGFP-R(L1) | CCTGCTCCTCCACCTCCGGCGGGAAGCCATGGCTCAACTTCTTGTACAGCTCGTCCATG | |
| O569 | O569 d2EGFP-R(L2) | ATCCCGCTCTCCTGGGCACAAGACATGGGCAGCGTGCCATCATCCTGCTCCTCCACCTC | |
| O570 | O570 d2EGFP-R(L3) | CACATTGATCCTAGCAGAAGCACAGGCTGCTGGGTGACGGTCCATCCCGCTCTCCTGG | |
| O571 | O571 d2EGFP-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTCTACACATTGATCCTAGCAGAAGC | |
| O572 | O572 CD8aSP-F(CE_p2Y | G3) | ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG |
| O573 | O573 EGFP-R(CE_mN1ce) | GGATTACTACCAACACAAGGCTTGTACAGCTCGTCCATGCC | |
| O574 | O574 mN1c-R(C) | CCTTCTTCTTTTTCGTGACAGCAAAACTCCGC | |
| O575 | O575 p2Y-F(C_HindIII) | AAGCTTCCCTCATTCAAGTACCAGTCTAGTCG | |
| O576 | O576 VP64-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTCAGagcatGtcCagGtcGaagtcatctagggcatcgg | |
| O577 | O577 Gal4-F(CE_m|hN1c) | TCACGAAAAAGAAGAAGGATGAAGCTGCTGAGCAGCatc | |
| O578 | O578 ZF21-16-F(CE_m|hN1c) | TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC | |
| O579 | O579 ZF42-10-F(CE_mN1c) | TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCAC | |
| O580 | O580 ZF43-8-F(CE_mN1c) | TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC | |
| O581 | O581 KRAB-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTCACACCAGCCAAGGTTCTTCGCccttttccagcc | |
| O582 | O582 rtTAA-F(CE_m|hN1c|e) | TCACGAAAAAGAAGAAGGatgtctagactggacaagagc | |
| O583 | O583 rtTAA-R(CE_p2Y-HindIII) | GTACTTGAATGAGGGAAGCTTTCAcccggggagcatgtcaag | |
| O584 | O584 mN1c-F-C(S) | CCAGTTGCACCTCATGTACG | |
| O585 | O585 mTagBFP2-R(L1) | CCTGCTCCTCCACCTCCGGCGGGAAGCCATGGCTCAACTTATTCAGCTTGTGCCCCAG | |
| O586 | O586 rtTAA-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCatgtctagactggacaagagcaaag | |
| O587 | O587 rtTAA-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTttacccggggagcatgtcaag | |
| O588 | O588 VP64-R(MfeI | CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCACAATTGtagcatatcCagatcaaagtcatctaggg |
| O589 | O589 ZF21-16-F(CE_P2A) | GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC | |
| O590 | O590 ZF42-10-F(M | CE_p2Y) | accggaacccttaatGAATTCcggccgcttcTAGAGaGACGCTG |
| O591 | O591 CMV-F(BP_M) | tGAATTCcggccgcttcTAGAGcgttacataacttacggtaaatg | |
| O592 | O592 CMV-R(BS_M) | cACCGGTCTGCAGacggccgcTACTAGTAagctctgcttatatagacctc | |
| O593 | O593 TetO-R(BS_M) | cACCGGTCTGCAGacggccgcTACTAGTATCTCTATCACTGATAGGGAgtaaactggat | |
| O594 | O594 EGFP-F(L1_palm) | CaaaatgcggctgctgcttcgcccgggaCccaccCgtcgccaccATGGTGAGCAAGGGC | |
| O595 | O595 EGFP-F(L2_palm) | Gggggtcgctgtgcctgtccccgtgtgatccgaaaagtgctggcaaaatgcggctgctg | |
| O596 | O596 palm-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCatgcggggggggcacaaagggggtcgctgtgc | |
| O597 | O597 mCherry-F(CE_mN1c-SV40NLS) | TCACGAAAAAGAAGAAGGCCAAAAAAGAAGAGAAAGGTAATGGTGAGCAAGGGCGAGG | |
| O598 | O598 mCherry-R(L_HA) | AGCGTAATCTGGTACGTCGTATGGGTACTTGTACAGCTCGTCCATGC | |
| O599 | O599 HA-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCAAGCGTAATCTGGTACGTCGTATG | |
| O600 | O600 mCherry(CE_mN1c) | TCACGAAAAAGAAGAAGGATGGTGAGCAAGGGCGAGG | |
| O601 | O601 mN1c-R(M_S1) | TCGAGTTCTGCAGCTGCAGCGCCACCACTCGTGCCCGG | |
| O602 | O602 mN1c-F(M_S1) | GCTGCAGCTGCAGAACTCGATCCTATGGACATACGGGGGTCC | |
| O603 | O603 mN1c-R(M_S2) | GTTCGCTTTTGTGCACTTCGATTTTATACGGAATATTCAAGCTCCCAAGAGAGGCC | |
| O604 | O604 mN1c-F(M_S2) | CGAAGTGCACAAAAGCGAACCTGTCGAACCGCC | |
| O605 | O605 CD8aSP-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG | |
| O606 | O606 PDGFRB-R | GCGAGGTTTCTTTTGCCAG | |
| O607 | O607 mCherry-F(CE_PDGFRB) | TGGCAAAAGAAACCTCGCATGGTGAGCAAGGGCGAGG | |
| O608 | O608 mCherry-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCACTTGTACAGCTCGTCCATGC | |
| O609 | O609 tTAA-F(CE_PDGFRB) | TCTGGCAAAAGAAACCTCGCATGAGTCGATTGGATAAATCTAAGGTAATTAACAG | |
| O610 | O610 minCMV-R(L1_ZF21-16) | cgcTACTAGTAtCACTTCTAAttCACTTCTAAtCTCTAgTAggcgatctgacggttcac | |
| O611 | O611 minCMV-R(L2_ZF21-16) | CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGTAtCACTTCTAAttCAC | |
| O612 | O612 minCMV-R(L1_ZF43-8) | cgcTACTAGTAgTCCTCACTCtgTCCTCACTCtCTCTAgTAGGCGATCTGACGGTTCAC | |
| O613 | O613 minCMV-R(L2_ZF43-8) | CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGTAgTCCTCACTCtg | |
| O614 | tTAA-R-N(S) | CCCCTCGATACCTACTTCGTTC | |
| O615 | O615 UAS-R(CE_minCMV） | CTCTAGTActagagtctccgctcggaggacaga | |
| O616 | O616 minCMV-F(CE_UAS) | cggagactctagTACTAGAGGGTAGGCGTGTACGGTGG | |
| O617 | O617 pSV40-R(L_ZF43-8) | cgcTACTAGTAgTCCTCACTCtgTCCTCACTCtCTCTAgTAtttgcaaaagcctaggcc | |
| O618 | O618 mNc F (OE-test) | ATTCTCGACTACAGTTTCACAGGAG | |
| O619 | O619 mNc R (OE-test) | CCTTCTTCTTTTTCGTGACAGCAAAA | |
| O620 | O620 LNRA-R | GCAGTCACCCCCGTCC | |
| O621 | O621 LNRB-F | TGCACCCAGTCCCTCCAAT | |
| O622 | O622 LNRA R (OE_AB lnkr-WT10) | GTTCTTCCACGGATCATTGAAGTTAAG | |
| O623 | O623 LNRB F (OE_AB lnkr-WT10) | TCACTTAACTTCAATGATCCGTGGAAG | |
| O624 | O624 LNR A R (OE_ABlnkr-WT 8) | CTTCCACGGATCATTGAAGTTAAGTG | |
| O625 | O625 LNR B F (OE_AB lnkr-WT8) | CTTAACTTCAATGATCCGTGGAAGAAC | |
| O626 | O626 LNR A R (OE_AB lnkr-WT6) | CCACGGATCATTGAAGTTAAGTGAG | |
| O627 | O627 LNR B F (OE_AB lnkr-WT6) | AACTTCAATGATCCGTGGAAGAAC | |
| O628 | O628 LNR A R (OE_AB lnkr-WT4) | CGGATCATTGAAGTTAAGTGAGCAG | |
| O629 | O629 LNR B F (OE_AB lnkr-WT4) | TTCAATGATCCGTGGAAGAACTGC | |
| O630 | O630 LNR A R (OE_AB lnkr-WT2) | ATCATTGAAGTTAAGTGAGCAGTCACC | |
| O631 | O631 LNR B F (OE_AB lnkr-WT2) | AATGATCCGTGGAAGAACTGCAC | |
| O632 | O632 LNR A-lnkr R(OE mut L-I ) | AGTTCTTCCACGGATCATTGAAGTTGATTGAGCAGTCACCCCCGT | |
| O633 | O633 LNR B-lnkr F(OE mut L-I ) | ACGGGGGTGACTGCTCAATCAACTTCAATGATCCGTGGAAGAACT | |
| O634 | O634 LNR A-lnkr R(OE mut L-V) | AGTTCTTCCACGGATCATTGAAGTTCACTGAGCAGTCACCCCCGT | |
| O635 | O635 LNR B-lnkr F(OE mut L-V ) | ACGGGGGTGACTGCTCAGTGAACTTCAATGATCCGTGGAAGAACT | |
| O636 | O636 LNR A-lnkr R(OE mut L-A) | AGTTCTTCCACGGATCATTGAAGTTGGCTGAGCAGTCACCCCCGT | |
| O637 | O637 LNR B-lnkr F(OE mut L-A ) | ACGGGGGTGACTGCTCAGCCAACTTCAATGATCCGTGGAAGAACT | |
| O638 | O638 LNR A-lnkr R(OE mut L-G) | AGTTCTTCCACGGATCATTGAAGTTGCCTGAGCAGTCACCCCCGT | |
| O639 | O639 LNR B-lnkr F(OE mut L-G ) | ACGGGGGTGACTGCTCAGGCAACTTCAATGATCCGTGGAAGAACT | |
| O640 | O640 LNR A-lnkr R(OE mut S-G) | GTTCTTCCACGGATCATTGAAGTTAAGGCCGCAGTCACCCCCGTCC | |
| O641 | O641 LNR B-lnkr F(OE mut S-G ) | GGACGGGGGTGACTGCGGCCTTAACTTCAATGATCCGTGGAAGAAC | |
| O642 | O642 LNR A-lnkr R(OE mut N-Q) | GCAGTTCTTCCACGGATCATTGAATTGAAGTGAGCAGTCACCCCC | |
| O643 | O643 LNR B-lnkr F(OE mut N-Q ) | GGGGGTGACTGCTCACTTCAATTCAATGATCCGTGGAAGAACTGC | |
| O644 | O644 LNR A-lnkr R(OE mut N-V) | GCAGTTCTTCCACGGATCATTGAAAACAAGTGAGCAGTCACCCCC | |
| O645 | O645 LNR B-lnkr F(OE mut N-V ) | GGGGGTGACTGCTCACTTGTTTTCAATGATCCGTGGAAGAACTGC | |
| O646 | O646 LNR A-lnkr R(OE mut S-A) | GTTCTTCCACGGATCATTGAAGTTAAGGGCGCAGTCACCCCCGTCC | |
| O647 | O647 LNR B-lnkr F(OE mut S-A ) | GGACGGGGGTGACTGCGCCCTTAACTTCAATGATCCGTGGAAGAAC | |
| O648 | O648 IL10-F(L1) | TGCTTACATGCGGAGATGTGGAGGAGAATCCGGGACCAATGCATTCTAGCGCTCTTTTG | |
| O649 | O649 IL10-F(L2) | GAAGGTAGGGGGTCATTGCTTACATGCGGAGATGTG | |
| O650 | O650 TetOn3G-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGAGCCGACTGGACAAG | |
| O651 | O614 mNc F(CE_LNR-A[-]hlx3+)√ | ATTCTCGACTACAGTTTCACAAATCACGCCTGCGGTT | |
| O652 | O615 LaG17 R(CE_G6-LNR-A[-]hlx3+) | ACCGCCTCCACCGCCTCCGGAAACGGTCACCTGTGTACC | |
| O653 | O616 mNc F(CE_G6-LNR-A[-]hlx3+) | GGAGGCGGTGGAGGCGGTAATCACGCCTGCGGTTG | |
| O654 | O617 mNc F(CE_G6-LNR-A[-]) | GGAGGCGGTGGAGGCGGTTCACTTAACTTCAATGATCCGTGGAAG | |
| O655 | O655 LNR A-lnkr R(OE mut L-I ) | TTGAAGTTAAGTGAGCAGTCACCCCCGTCCCAAC | |
| O656 | O656 LNR B-lnkr F(OE mut L-I ) | GACTGCTCACTTAACTTCAATGATCCGTGGAAGAACTGCACCC | |
| O657 | O657 LNR A-lnkr R(OE mut L-V) | TTGAAGTTCACTGAGCAGTCACCCCCGTCCCAAC | |
| O658 | O658 LNR B-lnkr F(OE mut L-V ) | GACTGCTCAGTGAACTTCAATGATCCGTGGAAGAACTGCACCC | |
| O659 | O659 LNR A-lnkr R(OE mut L-A) | TTGAAGTTGGCTGAGCAGTCACCCCCGTCCCAAC | |
| O660 | O660 LNR B-lnkr F(OE mut L-A ) | GACTGCTCAGCCAACTTCAATGATCCGTGGAAGAACTGCACCC | |
| O661 | O661 LNR A-lnkr R(OE mut L-G) | TTGAAGTTGCCTGAGCAGTCACCCCCGTCCCAAC | |
| O662 | O662 LNR B-lnkr F(OE mut L-G ) | GACTGCTCAGGCAACTTCAATGATCCGTGGAAGAACTGCACCC | |
| O663 | O663 LNR A-lnkr R(OE mut S-G) | TTGAAGTTAAGGCCGCAGTCACCCCCGTCCCAACC | |
| O664 | O664 LNR B-lnkr F(OE mut S-G ) | GACTGCGGCCTTAACTTCAATGATCCGTGGAAGAACTGCACCC | |
| O665 | O665 LNR A-lnkr R(OE mut N-Q) | TTGAACTGAAGTGAGCAGTCACCCCCGTCC | |
| O666 | O666 LNR B-lnkr F(OE mut N-Q ) | GACTGCTCACTTCAGTTCAATGATCCGTGGAAGAACTGCACCCAG | |
| O667 | O667 LNR A-lnkr R(OE mut N-V) | TTGAACACAAGTGAGCAGTCACCCCCGTCC | |
| O668 | O668 LNR B-lnkr F(OE mut N-V ) | GACTGCTCACTTGTGTTCAATGATCCGTGGAAGAACTGCACCCAG | |
| O669 | O669 LNR A-lnkr R(OE mut S-A) | TTGAAGTTAAGGGCGCAGTCACCCCCGTCCCAACC | |
| O670 | O670 LNR B-lnkr F(OE mut S-A ) | GACTGCGCCCTTAACTTCAATGATCCGTGGAAGAACTGCACCC | |
| O671 | O671 LNR A-lnkr R(OE mut N-L) | TTGAACAGAAGTGAGCAGTCACCCCCGTCC | |
| O672 | O672 LNR B-lnkr F(OE mut N-L ) | GACTGCTCACTTCTGTTCAATGATCCGTGGAAGAACTGCACCCAG | |
| O673 | O673 LNR A-lnkr R(OE mut N-G) | TTGAAGCCAAGTGAGCAGTCACCCCCGTCC | |
| O674 | O674 LNR B-lnkr F(OE mut N-G ) | GACTGCTCACTTGGCTTCAATGATCCGTGGAAGAACTGCACCCAG | |
| O675 | O675 tTAA-F(CE_p2A) | GAAGAAAACCCGGGACCTATGAGTCGATTGGATAAATCTAAGGTAATTAACAG | |
| O676 | O676 rtTAA-F(CE_p2A) | GAAGAAAACCCGGGACCTatgtctagactggacaagagcaaag | |
| O677 | O677 CD19ECD-F(3-BamHI) | CTGGGATCCGAAGAGCCTCTCGTCGTGAAAG | |
| O678 | O678 CD19ECD-R(TAA-EcoRI-3) | CGGGAATTCTTACTTCCATCCCCCCGTGC | |
| O679 | O679 Gal4-R(CE_VP64) | agaAcctccActTccAccagcagctgcCACTGTCAGCTGCCGCTGgc | |
| O680 | O680 VP64-F(CE_Gal4) | ggTggAagTggaggTtctggCgggAGcgatgctttagacgattttgacttagatatgc | |
| O681 | O681 VP64-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCAtgatccGagcatGtcCagGtcGaagtcatctaggg | |
| O682 | O682 Gal4-F(CE_P2A) | GAAGAAAACCCGGGACCTATGAAGCTGCTGAGCAGCatc | |
| O683 | O683 mCherry-F-C(S) | ACCTCCCACAACGAGGAC | |
| O684 | O684 SynTF-F-N(CE_P2A) | GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTG | |
| O685 | O685 mScarlet-F(3-SalI-Kozak-ATG-BamHI) | CTAGTCGACGCCACCATGGGATCCGTGAGCAAGGGCGAGG | |
| O686 | O686 mScarlet-R(M) | CTTGCGGTCcACGTTGTAGGCGCCG | |
| O687 | O687 mScarlet-F(M) | TACAACGTgGACCGCAAGTTGGACATCAC | |
| O688 | O688 G4S-R(C) | ACATCGGCCATTGAACCACCCCCACCGGAACC | |
| O689 | O689 LaG17-F(CE_G4S) | TGGTTCAATGGCCGATGTGCAGC | |
| O690 | O690 mN1c-F-N(C) | ATTCTCGACTACAGTTTCACAGGAGGAGCG | |
| O691 | O691 HA-R(C) | CGCGTAATCTGGTACATCATAGGGGTAGGG | |
| O692 | O692 LaG17-F(CE_HA) | GATGTACCAGATTACGCGATGGCCGATGTGCAGC | |
| O693 | O693 LaG17-R | GGAAACGGTCACCTGTGTACC | |
| O694 | O694 G4S-F(CE_LaG17) | ACACAGGTGACCGTTTCCGGGGGGGGTGGCAGTGG | |
| O695 | O695 SynZF-F(CE_p2Y-Kozak) | ATCACGTTAGGGCTAGTCGACGCCACCATGGCAAGTCCTAGGGTG | |
| O696 | O696 TRE3GV-F(1-BP_M_p2Y) | CGAATTCcggccgcttcTAGAGTCCCTATCagtgatagagaacgtatgaag | |
| O697 | O697 4xUAS-F(1-BP_M_p2Y) | tGAATTCcggccgcttcTAGAGcgccaagctacggg | |
| O698 | O698 4xUAS-R(1-BS_M_p2Y) | cACCGGTCTGCAGacggccgcTACTAGTAcgctcggaggacaga | |
| O699 | O699 CMV-R(L1_21-43) | TCTAAtCTAGTAgTCCTCACTCttCACTTCTAAtCTAGTAagctctgcttatatagacc | |
| O724 | O724 pol-F-C | GCCCTCAAAGTAGACGGC | |
| O725 | O725 ori-F-3 | CGCCTGGTATCTTTATAGTCCTG | |
| O726 | O726 tTAA-R(C) | TCCTGGGAGCATATCCAGGTCAAGATCGTC | |
| O727 | O727 CD8ASP-F(L1_P2A) | CTCCCTGCTGAAGCAAGCAGGAGACGTGGAAGAAAACCCGGGACCTATGGCGCTGCCGG | |
| O728 | O728 CD8ASP-F(L2_P2A | CE_tTAA) | CTGGATATGCTCCCAGGAggctccggcGCCACCAACTTCTCCCTGCTGAAGCAAGC |
| O729 | O729 LaG2-F-N(S) | GTGCTGCCTCCGGTC | |
| O730 | O730 tTAA-F-C(S) | GCTTAAGTGCGAGAGTGGC | |
| O731 | O731 ACD8b-F-N(S) | TATCCGGGGTCTCCCTCC | |
| O732 | O732 Gal4DBD-R-N(S) | ttggcgcacttgggc | |
| O733 | O733 TetOn3G-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGGAGGTgaattcGCCACC | |
| O734 | O734 TetOn3G-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTACCTCCggatccTTAGCC | |
| O735 | O735 T2A-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACggAtcAggTGAGGGTCGG | |
| O736 | O736 2.3D11scFv-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCACTTTATTTCGACTTTCGTGCCTCC | |
| O737 | O737 CAR3G-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACCCAGCCAAACCAACAACTACAC | |
| O747 | O747 CD8A-F(CE_P2A) | GAAGAAAACCCGGGACCTATGGCGCTGCCGG | |
| O748 | O748 SynPro_space_5-F(CE_p2Y) | accggaacccttaatGAATTCCGGGTTTCGTAACAATCGCATGAGGATTCGCAACGC | |
| O749 | O749 SynPro_space_7/7-F(CE_p2Y) | accggaacccttaatGAATTCGCCTAGATCATCGTTGGC | |
| O750 | O750 SynPro_space_6/7-F(CE_p2Y) | accggaacccttaatGAATTCCGCACGTATCACTTGATC | |
| O751 | O751 SynPro_space_5/7-F(CE_p2Y) | accggaacccttaatGAATTCCTTTCCACGATCATGTGC | |
| O752 | O752 SynPro_space_4/4-F(CE_p2Y) | accggaacccttaatGAATTCGACTGTTGCGAACGATTC | |
| O753 | O753 SynPro_space_3/4-F(CE_p2Y) | accggaacccttaatGAATTCTCCCGTCTCAGTAAAGG | |
| O754 | O754 SynPro_space_2/4-F(CE_p2Y) | accggaacccttaatGAATTCAATCGGACTGCCTTCG | |
| O755 | O755 SynPro_space_1/4-F(CE_p2Y) | accggaacccttaatGAATTCGTATCAGTCGCCTCGG | |
| O756 | O756 minCMV2-F(L_space3) | CGTAAGAGGCTCACTCTCCCTTACACGGAGTGGATAGGTAGGCGTGTACGG | |
| O757 | O757 minCMV2-R(CE_p2Y) | CAGTACAaTAAGACCACCGGTAAGTATAAGACAAAAGTGTTGTGG | |
| O758 | O758 ZF21.16_8/8-F(L) | GAGGATTCGCAACGCaTTAGAAGTGaGCCTAGATCATCGTTGGC | |
| O759 | O759 ZF21.16_7/8-F(L) | GCCTAGATCATCGTTGGCaTTAGAAGTGaCGCACGTATCACTTGATC | |
| O760 | O760 ZF21.16_6/8-F(L) | CGCACGTATCACTTGATCaTTAGAAGTGaCTTTCCACGATCATGTGC | |
| O761 | O761 ZF21.16_5/8-F(L) | CTTTCCACGATCATGTGCaTTAGAAGTGaGACTGTTGCGAACGATTC | |
| O762 | O762 ZF21.16_4/8-F(L) | GACTGTTGCGAACGATTCaTTAGAAGTGaTCCCGTCTCAGTAAAGG | |
| O763 | O763 ZF21.16_3/8-F(L) | TCCCGTCTCAGTAAAGGTaTTAGAAGTGaAATCGGACTGCCTTCG | |
| O764 | O764 ZF21.16_2/8-F(L) | AATCGGACTGCCTTCGTAaTTAGAAGTGaGTATCAGTCGCCTCGG | |
| O765 | O765 ZF21.16_1/8-F(L) | GTATCAGTCGCCTCGGAAaTTAGAAGTGaCGTAAGAGGCTCACTCTC | |
| O766 | O766 TetO_6/6-F(L) | CGCACGTATCACTTGATCTCCCTATCagtgatagagaCTTTCCACGATCATGTGC | |
| O767 | O767 TetO_5/6-F(L) | CTTTCCACGATCATGTGCTCCCTATCagtgatagagaGACTGTTGCGAACGATTC | |
| O768 | O768 TetO_4/6-F(L) | GACTGTTGCGAACGATTCTCCCTATCagtgatagagaTCCCGTCTCAGTAAAGG | |
| O769 | O769 TetO_3/6-F(L) | TCCCGTCTCAGTAAAGGTTCCCTATCagtgatagagaAATCGGACTGCCTTCG | |
| O770 | O770 TetO_2/6-F(L) | AATCGGACTGCCTTCGTATCCCTATCagtgatagagaGTATCAGTCGCCTCGG | |
| O771 | O771 TetO_1/6-F(L) | GTATCAGTCGCCTCGGAATCCCTATCagtgatagagaCGTAAGAGGCTCACTCTC | |
| O772 | O772 minCMV2-F(CE_p2Y) | accggaacccttaatGAATTCGGTAGGCGTGTACGGTGG | |
| O773 | O773 SV40NLS-KRAB-F(CE_P2A) | GAAGAAAACCCGGGACCTATGGGCCCAAAAAAGAAGAGAAAGGTAatggacg | |
| O774 | O774 KRAB-G4S-R | ACTACCTCCTCCACCTACAAGCCACGGTTCCTCGCccttttccagcctaag | |
| O775 | O775 TetR-F(CE_KRAB-G4S) | TTGTAGGTGGAGGAGGTAGTATGAGTCGATTGGATAAATCTAAGG | |
| O776 | O776 TetR-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCAAGAGCCACTCTCGCACTTAAG | |
| O777 | O777 Gal4-F(CE_KRAB-G4S) | TTGTAGGTGGAGGAGGTAGTATGAAGCTGCTGAGCAGCatc | |
| O778 | O778 Gal4-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCACACTGTCAGCTGCCGC | |
| O779 | O779 SynZFN-F(CE_KRAB-G4S) | TTGTAGGTGGAGGAGGTAGTATGGCAAGTCCTAGGGTG | |
| O780 | O780 ZF21.16-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTGTGTGTTTTCAGATGc | |
| O781 | O781 ZF42.10-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTGTGGGTTTTCAGATGc | |
| O782 | O782 ZF43.8-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTATGTGTTTTCAGATGtc | |
| O783 | O783 7xTetO-R(CE_CMVe) | accgtaagttatgtaacgGCCTACCTCGACATACG | |
| O784 | O784 4xUAS-F(CE_p2Y) | accggaacccttaatGAATTCcgccaagctacggg | |
| O785 | O785 4xUAS-R(CE_CMVe) | accgtaagttatgtaacgGTActagagtctccgc | |
| O786 | O786 8xZFO-R(CE_CMVe) | accgtaagttatgtaacgCACGCCTACCCTCTAG | |
| O787 | O787 VP64-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGgatgctttagacgattttgacttag | |
| O788 | O788 VP64-G4S-R | ACTACCTCCTCCACCAagcatGtcCagGtcGaagtcatc | |
| O789 | O789 SynZF-F(CE_VP64_G4S) | tgctTGGTGGAGGAGGTAGTatgGCAAGTCCTAGGGTG | |
| O790 | O790 CfaN-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCATGGCAACCCATCGACCTG | |
| O791 | O791 KRAB-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGgacgccaagagcctg | |
| O792 | O792 SynZF-F(CE_KRAB_G4S) | TTGTAGGTGGAGGAGGTAGTatgGCAAGTCCTAGGGTG | |
| O793 | O793 CfaC-F(CE_p2Y) | ATCACGTTAGGGCTAGTCGACGCCACCATGGTTAAGATCATATCACGG | |
| O794 | O794 SynZF-R(SV40NLS-CE_p2Y) | TACTTGAATGAGGGAAGCTTTCATACCTTTCTCTTCTTTTTTGGGGAGCCCTTCTCACC | |
| O795 | O795 KRAB-R(CE_NES) | GTCAATCTTTCCAGGGGCGGCAGTACAAGCCACGGTTCCTCGCccttttccagcctaag | |
| O796 | O796 SynZF-F(L1_dNLS) | GATCAGAGAACTTGTATTTTCAAAGTGGCAAGAAGAAGAAAATGGCAAGTCCTAGGGTG | |
| O797 | O797 SynZF-F(L2_dNLS) | GCCCCTGGAAAGATTGACGCTGAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC | |
| O798 | O798 KRAB-R | TACAAGCCACGGTTCCTCGCccttttccagcctaag | |
| O799 | O799 dNLS-F(CE_KRAB) | GAGGAACCGTGGCTTGTAAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC | |
| O800 | O800 KRAB-R(CE_TCS) | CAAGTTCTCTGATCCACCTACAAGCCACGGTTCCTCGCccttttccagcctaag | |
| O801 | O801 SynZF-F(CE_TCS) | GGTGGATCAGAGAACTTGTATTTTCAAAGTGGCGGAATGGCAAGTCCTAGGGTG | |
| O802 | O802 TEVp-F(L1_NLS) | GGTGCCAAAAAAGAAGAGAAAGGTAGGGGAGAGCCTGTTCAAAG | |
| O803 | O803 TEVp-F(L2_NLS) | ATCACGTTAGGGCTAGTCGACGCCACCATGGTGCCAAAAAAGAAGAGAAAGG | |
| O804 | O804 TEVpN-R(CE_CfaN) | CGTGTCGTATGAGAGGCATGTCTGAAAATTAGTGGTTACCAAAC | |
| O805 | O805 CfaN-F | TGCCTCTCATACGACACGGAAATTTTGACCGTC | |
| O806 | O806 CfaN-R(CE_p2Y) | GTACTTGAATGAGGGAAGCTTTCATGGCAACCCATCGACC | |
| O807 | O807 CfaC-F | ATCACGTTAGGGCTAGTCGACGCCACCATGGTTAAGATCATATCACGGAAGAGC | |
| O808 | O808 CfaC-R | GTTTGAAGCGACGAGACCATTTTTAAGGAGG | |
| O809 | O809 TEVpC-F(CE_CfaC) | GGTCTCGTCGCTTCAAACTGCGCCGAAAAGAGCATGTCCTCCATGG | |
| O810 | O810 BS-R(CE_minCMV2) | ACGCCTACCTCGACATACGTCACGCCTACCCTCTAG | |
| O811 | O811 minCMV2-F | ACGTATGTCGAGGTAGGCGT | |
| O812 | O812 VP64-R | CagcatGtcCagGtcGaagtcatc | |
| O813 | O813 dNLS-F(CE_VP64) | ttCgaCctGgaCatgctGAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC | |
| O814 | O814 SynZFC-R(CE_T2AN) | CCTACCTTCAccTgaTccGGAGCCCTTCTCACCTGT | |
| O815 | O815 SV40NLS-F(L1_T2A) | GCTTACATGCGGAGATGTGGAGGAGAATCCTGGACCAGTGCCAAAAAAGAAGAGAAAGG | |
| O816 | O816 T2A-F | ggAtcAggTGAAGGTAGGGGGTCATTGCTTACATGCGGAGATGTGG | |
| O817 | O817 TEVpC-R(NLS-CE_T2A) | CCTACCTTCAccTgaTccTACCTTTCTCTTCTTTTTTGGCTGGCTATAGACCAATTCG | |
| O818 | O818 VP64-F(L_T2A) | TACATGCGGAGATGTGGAGGAGAATCCTGGACCAgatgctttagacgattttgacttag | |
| O819 | O819 KRAB-F(L_T2A) | CTTACATGCGGAGATGTGGAGGAGAATCCTGGACCAATGgacgccaagagcc | |
| O824 | O824 CE_p2Y-SalI(CE_pSB1C3-XbaI) | gaattcgcggccgcttctagagATCACGTTAGGGCTAGTCGAC | |
| O825 | O825 CE_p2Y-HindIII(CE_pSB1C3-SpeI) | actgcagcggccgctactagtaGTACTTGAATGAGGGAAGCTT | |
| O826 | O826 CE_p2Y-EcoRI(CE_pSB1C3-XbaI) | gaattcgcggccgcttctagagaccggaacccttaatGAATTC | |
| O827 | O827 CE_p2Y-AgeI(CE_pSB1C3-SpeI) | actgcagcggccgctactagtaCAGTACAaTAAGACCACCGGT | |
| O828 | O828 mN1c-F(CE_BP) | gaattcgcggccgcttctagagATTCTCGACTACAGTTTC | |
| O829 | O829 mN1c-R(CE_BS) | actgcagcggccgctactagtaCCTTCTTCTTTTTCGTGA | |
| O830 | O830 mN1ce-F(CE_BP) | gaattcgcggccgcttctagagCCTTGTGTTGGTAGTAATCC | |
| O831 | O831 LaG2-F(CE_BP) | gaattcgcggccgcttctagagATGGCTCAGGTCCAGCTTG | |
| O832 | O832 LaG2-R(CE_BS) | actgcagcggccgctactagtaAACAGTGACTTGCGTTCCC | |
| O833 | O833 LaG16(CE_BP) | gaattcgcggccgcttctagagATGGCACAGGTCCAACTCG | |
| O834 | O834 LaG16-R(CE_BS) | actgcagcggccgctactagtaGCTTACCGTTACTTGTGTGCC | |
| O835 | O835 LaG17-F(CE_BP) | gaattcgcggccgcttctagagATGGCCGATGTGCAGC | |
| O836 | O836 LaG17-R(CE_BS) | actgcagcggccgctactagtaGGAAACGGTCACCTGTGTACC | |
| O837 | O837 ACD19-F(CE_BP) | gaattcgcggccgcttctagagGATATACAAATGACCCAGACTACGTCC | |
| O838 | O838 ACD19-R(CE_BS) | actgcagcggccgctactagtaTGATGAAACGGTTACGGATGTCC | |
| O839 | O839 CfaN-F(CE_BP) | gaattcgcggccgcttctagagTGCCTCTCATATGACACGG | |
| O840 | O840 CfaN-R(CE_BS) | actgcagcggccgctactagtaTGGCAACCCATCGACCTG | |
| O841 | O841 CfaC-F(CE_BP) | gaattcgcggccgcttctagagATGGTTAAGATCATATCACGGAAGAGC | |
| O842 | O842 CfaC-R(CE_BS) | actgcagcggccgctactagtaGTTTGAAGCTACGAGACCATTTTTAAGG | |
| O843 | O843 TEVN(CE_BP) | gaattcgcggccgcttctagagGGGGAGAGCCTGTTCAAAGG | |
| O844 | O844 TEVpN(CE_BS) | actgcagcggccgctactagtaTGTCTGAAAATTAGTGGTTACCAAACAAATG | |
| O845 | O845 TEVpC-F(CE_BP) | gaattcgcggccgcttctagagTGCGCCGAAAAGAGCATGTCCTCCATGGTTAG | |
| O846 | O846 TEVpC-R(CE_BS) | actgcagcggccgctactagtaCTGGCTATAGACCAATTCGTTCATC | |
| O847 | O847 SynZFN-F(CE_BP) | gaattcgcggccgcttctagagatgGCAAGTCCTAGGGTG | |
| O848 | O848 ZF21.16N-R(CE_BS) | actgcagcggccgctactagtaCTGGAAGGGCTTTTCTCCG | |
| O849 | O849 ZF21.16C-F(CE_BP) | gaattcgcggccgcttctagagTGTAGGATATGCATGCGAAACTTCTC | |
| O850 | O850 SynZFC-R(CE_BS) | actgcagcggccgctactagtaGGAGCCCTTCTCACCTGT |
gBlocks
> alphaCD19(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCTACCCCTATGATGTACCAGAT TACGCGGATATACAAATGACCCAGACTACGTCCAGCCTTTCCGCCTCCCTGGGTGATCGC GTGACAATCAGTTGTAGGGCTAGTCAGGACATAAGTAAATACCTCAACTGGTATCAACAG AAGCCTGACGGAACAGTTAAACTTCTTATCTATCATACAAGTAGGCTTCATTCAGGCGTT CCATCCAGATTTAGTGGATCTGGGAGCGGCACCGACTACTCACTCACGATTAGTAATCTG GAACAAGAGGACATCGCCACTTATTTTTGCCAGCAGGGTAACACTCTTCCCTATACATTC GGCGGCGGTACGAAACTCGAAATTACAGGGGGGGGTGGCAGTGGTGGCGGAGGTTCCGGT GGGGGTGGTTCAGAAGTAAAACTTCAGGAATCTGGTCCAGGGCTGGTTGCACCCAGCCAA TCCCTTTCCGTCACGTGCACCGTATCCGGGGTCTCCCTCCCAGACTACGGCGTGAGTTGG ATACGACAGCCGCCGCGCAAGGGGCTTGAGTGGCTGGGTGTCATTTGGGGATCTGAAACA ACGTATTATAATTCCGCTTTGAAATCCCGCCTCACTATTATCAAGGACAATTCTAAGAGC CAGGTCTTTCTGAAGATGAACAGCCTGCAAACGGATGACACAGCCATCTACTATTGTGCA AAGCACTATTACTATGGTGGTTCATACGCTATGGATTACTGGGGACAAGGGACATCCGTA ACCGTTTCATCACCCTGCGTCGGTAGCAACCC
> tTAA(CO) TGTCACGAAAGCGCCGACGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAGCGCAC TGGAGCTTCTGAACGAAGTAGGTATCGAGGGGCTCACCACACGAAAATTGGCACAGAAGC TCGGAGTGGAGCAGCCGACTTTGTACTGGCACGTTAAGAATAAGAGGGCTTTGCTTGACG CGCTGGCCATTGAGATGCTCGACCGGCATCACACTCACTTCTGCCCGCTTGAAGGCGAGA GTTGGCAAGATTTCCTTAGGAACAATGCAAAGAGCTTTCGATGCGCCCTGCTTAGCCATC GGGACGGCGCAAAGGTTCATCTTGGGACTCGGCCTACTGAGAAGCAATATGAAACCCTGG AAAACCAGCTTGCTTTCCTTTGCCAACAGGGGTTCTCACTGGAGAATGCCCTGTACGCAC TCTCCGCGGTCGGCCATTTTACTCTCGGGTGTGTTTTGGAAGATCAGGAACACCAGGTAG CGAAAGAGGAGCGAGAAACGCCTACAACTGATTCCATGCCACCGTTGCTGAGACAAGCTA TAGAGTTGTTCGACCACCAGGGTGCTGAACCGGCGTTTCTCTTTGGTCTGGAACTCATTA TCTGCGGCCTTGAAAAGCAGCTTAAGTGCGAGAGTGGCGGTCCCGCAGATGCACTTGATG ACTTTGATTTGGATATGTTGCCCGCGGACGCTCTTGATGACTTCGATCTGGATATGCTGC CGGCTGATGCCCTCGACGATCTTGACCTGGATATGCTCCCAGGATGAaagcttggaaccc ttaatataacttcgtat
> TetOn3G(CO) GGAGGTgaattcGCCACCATGAGCCGACTGGACAAGAGCAAGGTAATCAATTCAGCTTTG GAGCTTCTGAACGGCGTGGGAATCGAGGGTCTCACCACCAGAAAGCTCGCGCAAAAATTG GGGGTGGAACAACCGACTCTCTATTGGCATGTTAAGAACAAGAGGGCCCTCCTTGACGCA CTCCCCATCGAAATGCTGGATCGACATCACACACATAGTTGTCCGCTGGAGGGGGAGTCT TGGCAAGATTTCCTCAGGAATAACGCGAAGAGTTATCGGTGCGCTCTCCTTAGTCATCGC GATGGAGCGAAAGTGCATCTGGGGACAAGACCGACGGAAAAACAATATGAGACGTTGGAG AACCAACTGGCATTCTTGTGCCAACAGGGGTTCAGCCTTGAAAATGCTCTTTACGCCTTG AGCGCTGTTGGGCACTTCACCTTGGGCTGTGTACTGGAGGAGCAGGAGCACCAGGTCGCA AAGGAGGAGCGCGAAACGCCCACCACAGATAGTATGCCACCCCTGTTGAAGCAGGCTATC GAGCTTTTTGATCGGCAGGGTGCTGAGCCTGCCTTTTTGTTCGGCCTTGAGCTTATAATT TGCGGCCTGGAAAAACAACTCAAGTGCGAAAGCGGTGGTCCCACAGACGCATTGGACGAT TTTGATCTTGACATGCTGCCAGCCGATGCTTTGGACGATTTTGACCTGGATATGTTGCCG GCAGATGCCCTCGACGACTTTGATCTTGATATGTTGCCGGGCTAAggatccGGAGGT
> mN1ce(CO) CCCTGCGTCGGTAGCAACCCCTGCTACAATCAAGGGACCTGCGAGCCAACGTCAGAAAAT CCCTTTTATAGATGCCTGTGCCCGGCAAAGTTCAACGGACTGCTGTGCCACATACTCGAC TACAGTTTTACTGGGGGGGCGGGCCGGGATATACCCCCGCCTCAGATAGAAGAGGCATGC GAACTGCCGGAATGCCAGGTCGATGCGGGCAATAAAGTCTGCAACCTCCAATGCAATAAT CACGCCTGCGGTTGGGACGGGGGTGACTGCTCACTTAACTTCAATGATCCGTGGAAGAAC TGCACCCAGTCCCTCCAATGTTGGAAATATTTTTCCGATGGTCATTGTGATAGCCAGTGC AATTCCGCAGGATGTTTGTTCGATGGGTTTGACTGCCAACTGACAGAGGGTCAGTGTAAT CCTCTTTACGACCAATACTGCAAGGATCATTTCAGTGATGGACATTGTGACCAAGGTTGT AACAGTGCGGAGTGCGAATGGGATGGGTTGGATTGTGCTGAGCATGTTCCTGAGCGCCTC GCAGCGGGAACTTTGGTCCTCGTTGTCCTCCTTCCGCCTGACCAGCTCAGAAATAATAGC TTTCACTTTCTTAGAGAACTCTCTCATGTGTTGCATACCAACGTAGTCTTTAAGCGCGAC GCTCAAGGACAGCAAATGATCTTCCCGTACTATGGGCACGAAGAAGAGCTTCGGAAACAC CCGATAAAAAGGAGTACGGTTGGTTGGGCGACGAGTAGTCTTTTGCCGGGCACGAGTGGT GGCAGACAACGGCGGGAACTCGATCCGATGGACATACGGGGGTCCATAGTGTATCTGGAG ATTGACAACCGACAGTGTGTTCAAAGTTCAAGCCAGTGTTTTCAATCAGCCACTGATGTA GCTGCGTTTCTCGGAGCGTTGGCCTCTCTTGGGAGCTTGAATATTCCGTATAAAATCGAA GCTGTAAAAAGCGAACCTGTCGAACCGCCATTGCCAAGCCAGTTGCACCTCATGTACGTG GCCGCTGCTGCGTTCGTACTCTTGTTCTTCGTAGGCTGCGGTGTTTTGCTGTCACGAAAG CGCCGACGG
> hN1c(CO) CCCTGTCTTGGAGGGAATCCGTGTTACAACCAAGGGACATGTGAACCCACGAGCGAGTCT CCTTTTTACAGGTGTCTCTGTCCAGCAAAATTTAATGGATTGCTTTGCCACATTCTGGAT TACTCATTTGGTGGGGGAGCGGGGCGGGACATACCGCCGCCCCTTATTGAAGAAGCATGC GAACTGCCCGAGTGCCAGGAGGATGCAGGTAACAAGGTATGCTCTCTTCAGTGCAATAAC CACGCTTGCGGGTGGGATGGTGGCGATTGCTCCTTGAATTTTAATGATCCTTGGAAAAAT TGCACCCAATCACTTCAGTGTTGGAAATACTTCTCAGATGGCCATTGCGATTCTCAGTGT AACAGCGCGGGATGCTTGTTTGACGGATTCGACTGTCAAAGGGCTGAGGGACAATGTAAT CCGCTCTACGATCAGTATTGTAAAGACCATTTCAGCGACGGCCACTGTGACCAAGGCTGC AATAGTGCCGAGTGTGAGTGGGATGGGTTGGATTGCGCGGAGCATGTTCCGGAGAGACTT GCGGCAGGTACGCTCGTGGTTGTTGTACTCATGCCGCCTGAGCAACTGAGGAATAGTAGC TTTCACTTCCTCCGGGAACTGAGCCGGGTTCTGCATACTAACGTTGTATTTAAGAGAGAT GCACATGGTCAGCAAATGATATTTCCGTACTACGGTCGGGAAGAGGAATTGCGGAAGCAC CCCATTAAGAGAGCTGCCGAGGGTTGGGCCGCTCCGGATGCCCTCCTTGGACAAGTAAAA GCCTCCCTCCTTCCCGGCGGATCTGAGGGTGGACGGAGACGCCGGGAACTCGATCCAATG GATGTAAGAGGGTCCATTGTGTACTTGGAGATTGATAATCGCCAGTGTGTGCAGGCGTCC TCACAATGTTTTCAAAGTGCGACCGATGTCGCAGCATTCTTGGGTGCCCTGGCATCATTG GGCAGCCTGAATATTCCATATAAGATCGAAGCGGTGCAATCCGAAACGGTGGAACCGCCT CCACCGGCACAGCTTCATTTCATGTATGTCGCAGCAGCAGCTTTCGTTTTGCTTTTCTTC GTAGGGTGTGGTGTTCTCCTTAGTCGAAAACGACGCCGG
> LaG17(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG GAGGACCTCATGGCCGATGTGCAGCTCGTCGAGTCAGGTGGGGGTCTCGTACAAGCTGGG GGGTCACTCAGACTGTCTTGCGCCGCCAGTGGCAGAACCATCTCTATGGCAGCTATGTCC TGGTTCAGACAGGCGCCAGGCAAGGAGAGGGAGTTTGTGGCGGGTATAAGTAGGTCCGCA GGAAGCGCGGTCCACGCCGATTCAGTCAAAGGTCGGTTCACGATAAGCCGGGATAACACT AAGAACACTCTTTATCTCCAGATGAACTCTCTGAAGGCCGAGGATACGGCTGTTTACTAT TGCGCTGTACGGACATCTGGGTTCTTCGGGAGCATACCGCGCACCGGAACAGCGTTTGAT TACTGGGGACAAGGTACACAGGTGACCGTTTCCCCCTGCGTCGGTAGCAACCC
> LaG16-2(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG GAGGACCTCATGGCACAGGTCCAACTCGTCGAATCCGGAGGTCGCCTCGTGCAAGCTGGC GATTCACTTCGACTCTCTTGCGCGGCGTCAGGTCGGACCTTCAGCACGTCAGCTATGGCG TGGTTCCGCCAGGCGCCTGGGAGAGAGAGAGAGTTTGTAGCAGCCATCACATGGACTGTT GGAAATACTATATTGGGCGACTCCGTAAAAGGGCGCTTCACTATCTCACGAGATAGAGCG AAAAACACTGTAGATCTGCAAATGGACAATTTGGAGCCGGAGGATACTGCTGTGTATTAT TGCAGCGCTCGCTCTCGGGGGTACGTCCTCTCCGTACTCCGGAGCGTGGATTCATACGAC TACTGGGGACAGGGCACACAAGTAACGGTAAGCGGTGGGGGAGGGTCTGGTGGTGGAGGC TCAGGAGGCGGGGGATCTATGGCTCAGGTCCAGCTTGTAGAGAGCGGTGGTGGATTGGTG CAAGCAGGAGGGAGCCTCAGGCTTAGTTGTGCTGCCTCCGGTCGAACCTTCTCAAATTAT GCAATGGGTTGGTTTAGGCAAGCTCCTGGTAAAGAACGGGAGTTCGTGGCCGCAATTAGC TGGACGGGAGTTAGCACATATTACGCTGACTCTGTGAAAGGTCGGTTCACGATAAGTCGA GACAATGATAAGAATACGGTTTACGTCCAAATGAATAGCCTCATCCCGGAGGACACAGCT ATATATTATTGCGCTGCTGTACGGGCAAGATCCTTCTCAGATACTTATAGCAGGGTTAAC GAATACGATTATTGGGGGCAGGGAACGCAAGTCACTGTTCCCTGCGTCGGTAGCAACCC
> EGFPtm(CO) GGAAGAAAATCCCGGTCCGATGGCCCTTCCTGTAACGGCGCTCCTTCTCCCTCTTGCCC TCCTGCTCCATGCCGCACGACCAATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGG TGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCG AGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCA GCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCT ACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGG TGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGG AGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATA TCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCG AGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCC CCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCA ACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCG GCATGGACGAGCTGTACAAGAATGCCGTTGGTCAAGACACGCAGGAAGTCATTGTTGTAC CCCACTCCCTGCCATTTAAGGTGGTAGTAATTTCAGCCATCCTTGCTCTCGTTGTGTTGA CAATTATTTCTCTGATAATTCTTATCATGCTCTGGCAAAAGAAACCTCGCTGAaagcttg gaacccttaatataacttcgtat
> CD19ECD(CO) TTGCTCCCACTGGCCTTGCTGCTTCATGCGGCACGGCCTTACCCCTATGATGTACCAGAT TACGCGGAAGAGCCTCTCGTCGTGAAAGTTGAAGAAGGTGACAATGCAGTTTTGCAATGT CTGAAAGGGACGTCTGACGGACCAACCCAACAGCTCACTTGGAGTCGCGAATCCCCGCTT AAGCCGTTCCTGAAGTTGTCTCTGGGGTTGCCGGGTCTTGGTATACACATGCGCCCGCTT GCGATATGGCTCTTCATCTTCAATGTCAGCCAACAGATGGGCGGGTTCTATCTCTGTCAA CCGGGACCGCCATCAGAGAAGGCGTGGCAACCTGGTTGGACGGTAAACGTGGAAGGTAGC GGGGAGTTGTTTCGCTGGAACGTTTCTGATCTCGGGGGCCTGGGTTGCGGTCTGAAAAAT CGCTCTAGCGAAGGGCCGAGTTCACCTTCCGGCAAGCTCATGTCCCCGAAACTCTACGTG TGGGCGAAGGATAGGCCAGAAATTTGGGAGGGAGAACCCCCCTGCCTGCCGCCCAGAGAT TCACTCAACCAGAGTCTCTCCCAAGATCTGACTATGGCCCCAGGAAGTACCTTGTGGCTT AGCTGCGGTGTACCGCCCGACTCCGTGAGCCGAGGGCCTCTCTCTTGGACACACGTTCAC CCTAAGGGGCCGAAATCATTGTTGAGTTTGGAACTCAAAGACGATAGGCCAGCAAGGGAT ATGTGGGTTATGGAGACCGGCCTTCTGCTCCCACGAGCGACCGCTCAGGATGCGGGCAAG TATTATTGCCACAGAGGAAATCTGACTATGTCTTTTCATCTGGAAATTACTGCGCGGCCA GTTTTGTGGCACTGGCTGTTGCGCACGGGGGGATGGAAGGAGCAGAAATTGATCTCTGA
> Gal4DBD(CO) atgaagctgctgagcagcatcgagcaggcctgtgacatctgccggctgaagaaactgaag tgcagcaaagaaaagcccaagtgcgccaagtgcctgaagaacaactgggagtgccggtac agccccaagaccaagagaagccccctgaccagagcccacctgaccgaggtggaaagccgg ctggaaagactggaacagctgtttctgctgatcttcccacgcgaggacctggacatgatc ctgaagatggacagcctccaggacatcaaggccctgctgaccggcctgttcgtgcaggac aacgtgaacaaggacgccgtgaccgacagactggccagcgtggaaaccgacatgcccctg accctgcggcagcacagaatcagcgccaccagcagcagcgaggaaagcagcaacaagggc cagcggcagctgacagtgtct
> ZF21.16(CO) GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCTCCTCACGAGAGGCCTTTTCAATGT AGAATTTGTATGCGTAATTTTTCTaggaacttcatcctccagagaCATACAAGGACTCAC ACAGGTGAAAAGCCTTTTCAGTGCAGAATTTGTATGCGCAATTTTTCTcagggcggtaac ctggtgagaCATCTGCGTACCCACACTGGTGAAAAGCCTTTCCAATGTAGAATTTGTATG CGAAATTTTTCTcagcaaaccggcctgaacgtgCATCTGAAGACACACACAGGCGAGAAG GGATCC
> ZF42.10(CO) GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCACCACATGAAAGACCATTCCAATGT AGAATTTGTATGAGGAATTTTTCTaccggccagatcctggacagaCATACAAGGACCCAC ACCGGCGAAAAGCCATTCCAGTGTAGAATTTGTATGCGTAATTTTTCTgtggcccacagc ctgaagagaCATCTGAGGACCCACACCGGAGAGAAGCCTTTTCAATGCAGAATTTGTATG CGTAATTTTTCTgaccccagcaacctgagaaggCATCTGAAGACCCACACCGGAGAAAAG GGATCC
> ZF43.8(CO) GCTAGCCCCAGAGTGAGAACCGGTTCTAAGACACCTCCCCACGAGAGGCCTTTTCAGTGT AGAATTTGTATGCGTAATTTTTCTaggcaggacagactggacaggCACACAAGGACTCAC ACTGGTGAAAAGCCCTTCCAATGTAGAATTTGTATGCGCAATTTTTCTcagaaggagcac ctggccggcCATCTGAGGACCCACACAGGCGAAAAGCCTTTCCAGTGCAGAATTTGTATG AGAAATTTTTCTaggagagacaacctgaacagaCATCTGAAGACACATACAGGTGAAAAA GGATCC
> TEVp(FL)(CO) ATCACGTTAGGGCTAGTCGACGCCACCATGGGGGAGAGCCTGTTCAAAGGGCCCCGCGAT TATAACCCTATAAGCAGTACAATATGTCATCTGACTAACGAATCAGACGGACATACGACC TCCCTCTACGGGATAGGGTTCGGCCCCTTCATCATCACCAATAAGCACTTGTTTAGGCGA AATAACGGCACGCTTCTGGTACAGTCCCTGCACGGGGTCTTTAAGGTCAAGAATACGACA ACTCTCCAACAGCATCTTATTGATGGACGCGACATGATTATCATTAGAATGCCTAAAGAT TTTCCGCCGTTCCCACAAAAATTGAAGTTTAGAGAACCGCAAAGGGAAGAGCGCATTTGT TTGGTAACCACTAATTTTCAGACAAAGAGCATGTCCTCCATGGTTAGTGACACTTCATGC ACGTTTCCTTCTTCTGATGGTATATTTTGGAAACATTGGATACAGACGAAAGACGGACAG TGCGGGTCCCCTCTTGTCTCTACCAGGGACGGCTTTATCGTCGGCATACATTCAGCCAGT AATTTCACAAACACAAATAATTATTTTACTAGCGTGCCCAAAAATTTCATGGAGTTGCTG ACCAATCAAGAGGCACAGCAGTGGGTTTCTGGATGGAGACTCAACGCCGACAGCGTACTC TGGGGTGGACATAAGGTGTTCATGgtgAAACCCGAGGAACCCTTTCAGCCGGTGAAAGAG GCCACGCAACTGATGAACGAATTGGTCTATAGCCAGTGAAAGCTTCCCTCATTCAAGTAC
> ZF21_16N-CfaN-CfaC-ZF21_16C(CO) ATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGCGGACCGGATCAAAG ACGCCGCCTCACGAACGACCATTTCAATGCAGGATCTGCATGCGGAACTTCTCCCGGAAC TTTATCCTCCAGCGGCATACACGGACCCATACCGGAGAAAAGCCCTTCCAGTGCCTCTCA TATGACACGGAAATTTTGACCGTCGAATATGGCTTTCTTCCTATCGGGAAGATCGTTGAA GAAAGAATTGAATGCACGGTCTATACCGTGGATAAGAATGGTTTTGTATACACTCAACCG ATAGCGCAGTGGCATAACAGGGGGGAGCAAGAAGTCTTTGAATACTGTTTGGAGGATGGG TCCATCATCAGGGCAACCAAGGACCATAAGTTCATGACCACTGATGGGCAGATGCTTCCG ATTGATGAAATTTTCGAAAGGGGCCTTGACCTGAAGCAGGTCGATGGGTTGCCAATGGTT AAGATCATATCACGGAAGAGCCTGGGGACACAGAATGTGTATGATATTGGAGTAGGCGAG CCGCACAACTTCCTCCTTAAAAATGGTCTCGTAGCTTCAAACTGTAGGATATGCATGCGA AACTTCTCCCAGGGGGGCAACTTGGTCAGGCACCTTCGGACGCATACCGGAGAGAAGCCA TTTCAGTGCAGGATTTGCATGCGAAATTTTTCTCAGCAAACCGGGTTGAATGTACATCTG AAAACGCATACAGGTGAGAAGGGCTCCTGAAAGCTTCCCTCATTCAAGTAC
Abstract
Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.