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<!doctype html> KCL iGEM

3' Suspects Investigating Untranslated regions on 3' end

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what are we doing?

We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).

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Some of Our Work

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iGEM Symposium

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Bexley School Visit

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Social Media

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Lab Work

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