Team:WLC-Milwaukee/Experiments
Wet Lab
Our Wet Lab efforts focused on first designing our parts in such a way as to test new versions of past WLC-iGEM binding proteins for optimization and use in our test kit. Our work then turned to successfully sub-cloning our new parts (BBa_K2589000, BBa_K2589002 ) from the pETail4 plasmid containing the lambda phage genome into both pTrc99a and pSB1C3 along with improving a past part BBa_K2452002 to create the composite part BBa_K2589001. Our final focus in WetLab was to purify the proteins from our new parts using Nickel column purification (as most of our parts contain a His tag element) and then confirming expression or protein presence.
Part Design
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Cloning
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Protein Expression
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