Team:NUS Singapore-Sci/enzyme kinetics
Enzyme
Kinetics
Enzyme Kinetics and Least Squares Regression
The efficiency of our RESCUE system is likely to be dependent on multiple factors such as mismatch distance, length of spacer regions as with ADAR-dCas13b constructs (Cox et al., 2016), as well as the relative concentrations of the substrates/enzymes. The concept of regression models can be utilized to identify and evaluate the significance of these factors from experimental results. As such, a early build of an enzyme kinetics regression model on dCas13b-APOBEC editing efficiency may help us to gain further insights of the RESCUE system.