Team:IISER-Kolkata/Parts
Parts
Team IISER Kolkata’s contribution to this year’s 2018 iGEM registry are the following parts:
| Part Number | Part Type | Sequence | Experience |
|---|---|---|---|
| Bba_K2773000 | Composite | Confirmed | Will work, requires testing |
Bba_K2773000 is a composite biobrick part designed by Mr. Diptatanu Das and Miss. Hrishika Rai. The part comprises of an Arsenic responsive promoter and autorepressor unit (Bba_J33201) upstream of a strong ribosome binding site (Bba_B0030).
When cloned upstream of a reporter or any other protein coding gene, the part will cause strong expression of the protein in response to arsenate and/or arsenite ions. As per characterization by team Edinburgh 2006, the threshold for derepression of the promoter is 1μM of arsenate or arsenite salts in the external growth media of the chassis organism.