Basic Parts
A basic part is a functional unit of DNA that cannot be subdivided into smaller component parts. BBa_R0051 is an example of a basic part, a promoter regulated by lambda cl.
Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are many opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts.
BBa_K2837000 short
===Name: ===
Phenylpropanoyltransferase (BAPT, TAX7)
===Reaction: ===
baccatin III + (3R)-3-amino-3-phenylpropanoyl-CoA → N-debenzoyl-(3'-RS)-2'-deoxytaxol + coenzyme A
===Km: ===
5.6 uM for (3R)-3-amino-3-phenylpropanoyl-CoA;
68 uM for baccatin III;
2.4 uM for baccatin III and 4.9 uM beta-phenylalanoyl-CoA (Thornburg, 2015)
===Kcat: ===
0.0583 ± 0.0010 s
-1 (Thornburg, 2015)
===Enzyme Description: ===
BAPT is the enzyme that adds the C13 Taxol side chain partially formed to the Taxol backbone. It makes use of
CoA chemistry to add the side chain to the 13’ hydroxyl group of baccatin III.
BBa_K2837000 SequenceAndFeatures
===Design Notes===
Part digested from vector designed by Duke's 2016 team. Original vector constructed by Gibson Assembly of BAPT Gblock into pSMART-HC-Amp vector.
===Source===
Origin from Pacific Yew Tree, Taxus brevifolia
===References===
Thornburg, C. (2015). Development of a four-step semi-biosynthesis of the anticancer drug paclitaxel and its analogues (Doctoral dissertation, Michigan State University).
