Team:Kyoto/Discussion

Team:Kyoto/Project - 2018.igem.org

仲里 一応 訳中
1)Summary of our research

We worked on the construction of the yeast “Swallowmyces cerevisiae” which absorbs NaCl from solution and adjusts the salt concentration of the solution. We created a gene-disrupted strain designed to make Na+ inside the cell, expressed the chaperone and produced the compatible solute to reduce the damage the cell receives from high salt concentration. And, we added a variety of genes for taking Na+ into the vacuole to the collection of BioBrick parts. With the help of mathematical modeling optimizing the system, eventually we produced a yeast that retains averagely 80mM of Na+ into the cell. Also, we performed the model experiment that this device really absorbed Na+ from all over the solution and demonstrated the decrease of Na+ in solution by it.

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