Team:Kyoto/Discussion

Team:Kyoto/Project - 2018.igem.org

1)Summary of our research

We worked on the construction of the yeast “Swallowmyces cerevisiae” which absorbs NaCl from solution and adjusts the salt concentration of the solution. We created a gene-disrupted strain designed to make Na+ inside the cell, expressed the chaperone and produced the compatible solute to reduce the damage the cell receives from high salt concentration. And, we added a variety of genes for taking Na+ into the vacuole to the collection of BioBrick parts. With the help of mathematical modeling optimizing the system, eventually we produced a yeast that retains averagely 80mM of Na+ into the cell. Also, we performed the model experiment that this device really absorbed Na+ from all over the solution and demonstrated the decrease of Na+ in solution by it.

2)Performance and application of "Swallowmyces cerevisiae"

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Reference
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