BBa_K2843000 This part is an improvement of the 2017 East Chapel Hill iGem "CHOP" operon. At fluoride concentrations of 2uM and above, the delta-CRCB E coli strain (strain JW0619-1 from the Yale Coli Genetics Stock Center) will grow on chloramphenicol. This vector is designed with Xhol cutting sites so one may easily remove and replace different segments of the operon to screen for combinations of riboswitch and promoter with highest effectivity.
BBa_K2843002
This is BBa_K2843000 operon with the "Fluoride Binding Mutant", which is a mutant of the fluoride RBS. This serves as a controlled experiment because if there is growth of delta-CRCB Ecoli on the chloramphenicol plates than this indicates that growth of delta-CRCB is not entirely dependent only on the presence of fluoride.
