Team:Kyoto/Parts Collection

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This Part Collection enables to create a biological desalination yeast with aggregation system. BBa_K2665018, BBa_K2665019 and BBa_K2665020 work for yeast aggregation. BBa_K2665011 and BBa_K2665012 can be used for raising yeasts' halotorelance, and parts of BBa_K2665013 to BBa_K2665016 enhance yeasts' ability of Na+ uptake. BBa_K2665011 to BBa_K2665016 include a constitutive yeast promoter and a common terminator so iGEMers can use them without assembly to express these proteins in yeast.

Basic Parts

Number	parts	Remark	Designer	Remark2	Subimittion
BBa_K2665000	TDH3 promoter	－	Tong	－	not submitted
BBa_K2665001	CYCterm fused with 6xHis tag and stop codon	－	Tong	－	not submitted
BBa_K2665002	Mangrin ORF	－	Tong	－	not submitted
BBa_K2665003	ZrGPD1 ORF	－	Tong	－	not submitted
BBa_K2665004	AtNHXS1 ORF	－	Tong	－	not submitted
BBa_K2665005	SseNHX1 ORF	－	Tong	－	not submitted
BBa_K2665006	AVP1 ORF	including the stop codon	Tong	－	not submitted
BBa_K2665007	AtHKT1 ORF	－	Tong	－	not submitted
BBa_K2665008	ZrFPS1 ORF	－	Tong	－	not submitted
BBa_K2665018	Fgb	－	Tong	Fluorescence Microscope	"submitted 2A"
BBa_K2665019	SdrG	－	Tong	Fluorescence Microscope	"submitted 2B"
BBa_K2665022	Stalk	－	Tong	Fluorescence Microscope	not submitted
BBa_K2665023	ybbr-tag	Refer to the following K2665018, K2665019	Tong	－	not submitted
BBa_K2665024	ddFLN4	Refer to the following K2665018, K2665020	Tong	－	not submitted
BBa_K2665025	SED1 secretion signal	－	Tong	－	not submitted
BBa_K2665026	SED1 anchoring domain	－	Tong	－	not submitted

Number	parts	Remark	Designer	Experimental　data	Subimittion
BBa_K2665011	TDH3-Mangrin-6xHis-CYC	－	Tong	Spot Assay and Boil method	"submitted １A"
BBa_K2665012	TDH3-ZrGPD1-6xHis-CYC	－	Tong	〃	"submitted １B"
BBa_K2665013	TDH3-AtNHXS1-6xHis-CYC	－	Tong	〃	"submitted １C"
BBa_K2665014	TDH3-SseNHX1-6xHis-CYC	codon optimized	Tong	〃	"submitted １E"
BBa_K2665015	TDH3-AVP1-6xHis-CYC	not excluded the stop codon	Tong	〃	"submitted １D"
BBa_K2665016	TDH3-AtHKT1-6xHis-CYC	－	Tong	〃	"submitted１F"
BBa_K2665017	TDH3-ZrFPS1-6xHis-CYC	－	Tong	〃	not submitted
BBa_K2665020	Fgb-stalk	－	Tong	〃	"submitted 2C"
BBa_K2665021	SdrG-stalk	－	Tong	〃	not submitted