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PCR

The result of PCR is shown in Fig.1. All PCR was performed by primers in table 1. All PCR were succeeded. We used PrimeSTAR Max premix for amplification.

Fig.1 PCR of BBa_K1491033

Fig.1 PCR of Seq.1

Fig.1 PCR of Seq.2

Cell-free expression

Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence.