Team:Gifu/Results

HOME

TEAM

Team Members

Collaborations

PROJECT

Description

Experiments

Protocol

InterLab

Results

Improve

Attributions

Calendar

Reference

PARTS

SAFETY

HUMAN PRACTICES

Human Practices

Education & Engagement

JUDGING FORM

Results

---

PCR

The result of PCR is shown in Fig.1. All PCR was performed by primers in table 1. All PCR were succeeded. We used PrimeSTAR Max premix for amplification.

Fig.1 PCR of BBa_K1491033

Fig.1 PCR of Seq.1

Fig.1 PCR of Seq.2

Cell-free expression

Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence.

Fig.4 Expression in PURE system (No UV)

P: Positive (DHFR gene), N: Negative (Water), tem.: Template DNA

mRFP tem. and mRFP PCR was slightly turned into red.

Fig.4 Expression in PURE system (No UV)

P: Positive (eGFP), N: Negative (Water)

Positive (eGFP) shows green color.