Team:IIT Kanpur/Results

• Swash
  Home NEED and Background idea
• Project
  Design Results Safety References
• Modelling
  Model Overview
• Outreach
  Education and
  Public Engagement Integrated Human
  Practices Collaborations
• Parts and
  Procedures
  Biobricks Notebook Protocols InterLab
• Measure
  Measurement
• Team
  People Attributions

Results

Cloning of our device for optimizing extracellular expression using PelB/Ompt secretion tags under different Anderson promoters:

We used pSBIK3.m1 and pSB1C3 for cloning of our designed constructs. We synthesised our DNA construct via IDT. We added restriction enzyme site for Ecor1 and Pst1 in our synthesised double stranded DNA. We performed restriction enzyme digestion for both our double stranded DNA and backbones with Ecor1 and Pst1. These were ligated together.

These are the biobricks we submitted in iGEM registry.
BBa_K2874000
BBa_K2874001
BBa_K2874002

These are the sequences which we cloned but are not submitted with iGEM registry.
pBS1K3.m1 ( JA23100->pelB-LINKER-sfGFP-HIS-STOP)
pBS1K3.m1 ( JA23102->ompT-LINKER-sfGFP-HIS-STOP)

• 
• 
• 
• 
• 
•