We used general protocols as well as our own protocols for testing our gene and gathering quantitative data. You can find all of them below.
General ProtocolsOur general protocols come from the Lab Protocol Manual from the 2016 University of Lethbridge Synthetic Biology Workshop 2016.
Below is a copy of our lab protocol manual:
Our mentor, Robert, helped us adapt the lipase assay from Gupta et. al..
Below is our modified version:
Using our above Proof of Concept Protocol, we modified it slightly to test the same reaction but getting quantitative data from the spectrophotometer as the reaction progresses. We did this with help from the University of Calgary, specifically Cassie Stillner.
Note: We added 25uL of 50mM IPTG to a 50mL culture.
Below is our modified version: