Team:Bio Without Borders/Design

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Creating Factor C

We used an NEBuilder kit to combine two sequence that IDT sent us.

Creating a linker protein of the cellulose-binding domain (CBD) and GFP

This functions as the reporter protein which would demonstrate Factor C's ability to cleave.

Expressing Factor C through Pichia pastoris

After making our parts, we wanted to express the parts in an easy model organism like pichia.