Team:Bio Without Borders/Design

• Home

• TEAM

  • Team Members

  • Collaborations

• PROJECT

  • Description

  • Design

  • Notebook

  • InterLab

  • Model

  • Results

  • Demonstrate

  • Improve

  • Attributions

• PARTS

  • Parts Overview

  • Basic Parts

  • Composite Parts

  • Part Collection

• SAFETY

• H. PRACTICES

  • Human Practices

  • Education & Engagement

• AWARDS

  • Applied Design

  • Entrepreneurship

  • Hardware

  • Measurement

  • Model

  • Plant

  • Software

• JUDGING FORM

Creating Factor C

We used an NEBuilder kit to combine two sequences of the Limulus polyphemus that IDT sent us, as the whole sequence was too large to send as one part. We first confirmed that we could at the j04450 insert into the pSB1C3 backbone for submission. Once we confirmed this process, we then used the NEBuilder kit to put together the two G-Blocks of the Factor C sequence inside the pSCB1C3 backbone.

Creating a linker protein of the cellulose-binding domain (CBD) and GFP

This functions as the reporter protein which would demonstrate Factor C's ability to cleave.

Expressing Factor C through Pichia pastoris

After making our parts, we wanted to express the parts in an easy model organism like pichia.