System Design

Introduction

The goal of our project is to produce a quick, easy, and cheap method of detecting E. coli in a water sample. This has a large number applications in industry and at home. To create a product, we are exploiting the mechanism that allows bacteriophages to bind specifically to E. coli. The portion of the J-protein from Lambda phage that is necessary for binding to E. coli was cloned and Histidine-tagged. This will allow us to purify the protein and conjugate the horseradish peroxidase enzyme to it. We can then expose water samples containing varying concentrations of E. coli to the HRP-conjugated J-protein (J-protein-HRP) and determine how much binds, thereby telling us how much E. coli is present, by measuring HRP activity.

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Team:WLC-Milwaukee/Applied Design

System Design

Introduction