Team:Lethbridge HS/Experiments
Lethbridge HS
PROJECT
Description
Design
Model
Results
Demonstrate
Applied Design
PARTS
Parts Overview
Basic Parts
Composite Parts
Improve
LAB
Experiments
Notebook
Interlab
HUMAN PRACTICES
Human Practices
Engagement
TEAM
Members
Collaboration
Attributions
Sponsors
SAFETY
EXPERIMENTS
PROTOCOLS
Agarose Gel Extraction
Colony PCR
LB Media
DNA Purification from Enzymatic Reactions
Ligation Reactions
Ligation to Insert DNA to Vector DNA
PCR Amplification
PCR Product Purification
Phenol/Chloroform Extraction and Ethanol Precipitation
Phusion PCR Protocol
pJET Cloning
Plasmid Miniprep
Preparation of Chemically Competent Cells
Protein Overexpression
Restriction Enzyme Digest and Dephosphorylation
Transformation