PCR
- Before cycles: 98°C 10
- After cycles: 72°C 7min; 16°C ∞ (4°C is more suitable for storing PCR product, but 4°C overnight may damage some PCR machine) (A) For reactions in which the quantity of template is 200 ng / 50 μl or less:*
- -98°C 10 sec.
- -55°C 5 sec. or 15 sec
- -72°C 5 sec./kb
- -30-35 cycles
- (B) For reactions in which the quantity of template exceeds 200 ng / 50 μl:* -98°C 10 sec.
- -55°C 5 sec. or 15 sec.
- -72°C 30 - 60 sec./kb
- -30-35 cycles
Agarose Gel Electrophoresis
After setup the program doesn't start