Description
When the concentration of Fe3+ in the environment is high, the hard skeleton of the microenvironment of the iron bacteria will be destroyed and lose its protection. At this time, the added bactericide will have a greater killing effect on the bacteria. The bactericide we selected was cecropin AD, a cationic chimeric antimicrobial peptide obtained from cecropin, consisting of the first 11 amino acid residues of cecropin A and the last 26 amino acid residues of cecropin D. It has strong antibacterial activity against Gram-positive and negative bacteria.
Design
![](https://static.igem.org/mediawiki/2018/b/b2/T--ECUST--BiocideF1.png)
Result
We insert fragment of Cecropin AD into vector pET-28a(Figure 1)
![](https://static.igem.org/mediawiki/2018/9/9e/T--ECUST--result--biocide_1.jpg)
The plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR(Figure 2).
![](https://static.igem.org/mediawiki/2018/4/47/T--ECUST--result--biocide_2.jpg)
We verified the expression of Cecropin AD by SDS-PAGE(Figure 3).
![](https://static.igem.org/mediawiki/2018/4/46/T--ECUST--result--biocide_3.jpg)
To test the effect of cecropin AD, we measured the growth curve of iron bacteria adding with different concentration of cecropin AD.
![](https://static.igem.org/mediawiki/2018/2/2a/T--ECUST--B4.jpg>
<figcaption><b>Figure 4. Growth curve of iron bacteria adding with different concentration of cecropin AD
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