Figure 1. (a)Bacteria is screened from rusty iron sample and cultured with Winogradsky culture medium. With rust and biofilm, bacterial colony is bronzing and circular. (b)Gram Straing：Gram-negative bacteria. (c)Iron bacteria with its biofilm (d)Electron microscope picture of iron bacteria with rust and biofilm complex.Figure 2. The iron bacteria belongs to Pseudomonas sp. According to the identification results of 16s rDNAFigure 3: Iron bacteria growth curve.Bacteria is cultured in Winogradsky culture medium at 30℃ and shake at 220rpm, 2% inoculum of culture solution (culturing age 3 days) and measure the light absorption at 600 nm.
QUORUM SENSING
We constructed a quorum-sensing system and found that the promoter with afe-box can work when the AHL concentration is more than 10-7 mol/L.
Figure 4. (a) Fluorescence intensity at different time induced by different concentrations of AHL.
(b)A three-dimensional map with log10 concentration of AHL,time and fluorescence intensity as the coordinate
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RUST REMOVER
Figure 5. Fitting curves of oxalic acid and enterobactin
(a) 3D fitting figure of rust removal rate for oxalic acid ;(b) 3D fitting figure of rust removal rate for enterobactin; (c) fitting curve of rust removal rate for oxalic acid ;(b) fitting curve of rust removal rate for enterobactin.
