Team:ECUST/Demonstrate

Demonstrate

CULTURE AND IDENTIFICATION OF IRON BACTERIA

Figure 1. (a)Bacteria is screened from rusty iron sample and cultured with Winogradsky culture medium. With rust and biofilm, bacterial colony is bronzing and circular. (b)Gram Straing：Gram-negative bacteria. (c)Iron bacteria with its biofilm (d)Electron microscope picture of iron bacteria with rust and biofilm complex.
Figure 2. The iron bacteria belongs to Pseudomonas sp. According to the identification results of 16s rDNA
Figure 3: Iron bacteria growth curve.Bacteria is cultured in Winogradsky culture medium at 30℃ and shake at 220rpm, 2% inoculum of culture solution (culturing age 3 days) and measure the light absorption at 600 nm.

QUORUM SENSING

We constructed a quorum-sensing system and found that the promoter with afe-box can work when the AHL concentration is more than 10-7 mol/L.

Figure 4. (a) Fluorescence intensity at different time induced by different concentrations of AHL. (b)A three-dimensional map with log10 concentration of AHL,time and fluorescence intensity as the coordinate

Click here for detailed information.

RUST REMOVER

We have not successfully constructed the recombinant plasmid with enterobactin because the gene cluster is too long.But we purified the enterobactin from E. coli successfully and tested the rust removal effect.

Figure 5. Fitting curves of oxalic acid and enterobactin (a) 3D fitting figure of rust removal rate for oxalic acid ;(b) 3D fitting figure of rust removal rate for enterobactin; (c) fitting curve of rust removal rate for oxalic acid ;(b) fitting curve of rust removal rate for enterobactin.

Click here for detailed information.

BIOFILM REMOVER

FUR INVERTER

LYSIN

BIOCIDE

LIGHT-ON SUICIDE

IRON BACTERIA-KILL CONPREHENSIVE CHARACTERIZATION