Team:Vilnius-Lithuania/Composite

Composite Parts

Making Them Stronger

Composite pats that we created are used to build beta-barrel bearing protein synthesis and membrane insertion system. These parts encode recombinant proteins, that are are targeted to lipid bilayers to display different classes of antibodies.

Composite Parts

Part Number

Type

Name

Brief description

Length, nt

BBa_2622025

Composite

GJ6-His-GFP

Recombinant N-terminus histidine tagged GFP with temperature-sensitive non-coding RNA 3

809

BBa_2622026

Composite

Lpp-OmpA-His

Recombinant C-terminus histidine tagged OmpA fused with Lpp

597

BBa_2622027

Composite

Mstx-Lpp-OmpA-His

Recombinant C-terminus histidine tagged OmpA fused with Mistic and Lpp

933

BBa_2622028

Composite

Mstx-Lpp-OmpA-GFPN

Recombinant protein OmpA fused with Mistic, Lpp and GFP Nanobody

1272

BBa_2622029

Composite

Lpp-OmpA-GFPN

Recombinant protein OmpA fused with Lpp and GFP Nanobody

936

BBa_2622030

Composite

Lpp-OmpA-scFv_antiVLY

Recombinant protein OmpA fused with Lpp and scFv_antiVLY

1317

BBa_2622031

Composite

Mstx-Lpp-OmpA-scFv_antiVLY

Recombinant protein OmpA fused with Mistic, Lpp and scFv_antiVLY

1653

BBa_2622032

Composite

His-IgA-Mstx

Recombinant N-terminus histidine tagged IgA protein fused with Mistic

2001

BBa_2622033

Composite

His-scFv_antiVLY

N-terminus histidine tagged scFv_antiVLY

927

BBa_2622034

Composite

GFPN-IgA-Mstx

Recombinant protein IgA fused with GFP Nanobody and Mistic

2286

BBa_2622035

Composite

scFv_antiVGY-IgA-Mstx

Recombinant protein IgA fused with scFv_antiVLY and Mistic

2667

BBa_2622036

Composite

Sw11-OmpA-His

C-terminus histidine tagged OmpA with temperature-sensitive non-coding RNA 11

598

BBa_2622037

Composite

Sw11-Mstx-OmpA-His

Recombinant C-terminus histidine tagged OmpA fused with temperature-sensitive non-coding RNA 11 and Mistic

1082

BBa_2622038

Composite

Mstx-scFv_antiVLY

Recombinant protein scFv_antiVLY fused with Mistic

1266

BBa_2622039

Composite

BamA-His composite

C-terminus histidine tagged BamA

2569

BBa_2622040

Composite

BamA composite

C-terminus histidine tagged BamA controlled by T7 promoter and terminator

2580

BBa_2622041

Composite

BamA composite

BamA controlled by T7 promoter and terminator

2628

BBa_2622042

Composite

BamB composite

BamB controlled by T7 promoter and terminator

1369

BBa_2622043

Composite

BamC composite

BamC controlled by T7 promoter and terminator

1165

BBa_2622044

Composite

BamD composite

BamD controlled by T7 promoter and terminator

865

BBa_2622045

Composite

BamE composite

BamE controlled by T7 promoter and terminator

556

BBa_K2 BBa_2622046622046

Composite

SurA composite

SurA controlled by T7 promoter and terminator

1482

BBa_2622047

Composite

Mstx-OmpA_full-His

Full length recombinant C-terminus histidine tagged protein OmpA fused with Mistic

1340
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