Using thermodynamic data and correlate it with the sequence of the promoter region. The experimental and thermodynamic data of the sequences obtained can be used to correlate it with the expression levels of the protein. Come up with a model to understand the correlation between the sequence of promoter and expression rates.
Troubleshooting or try to understand the reason of extremely high expression rate of P8S.
Characterize remaining biobrick parts.
Test promoter library in other chassis such as E. coli and other gram-negative microorganisms
Use Codon usage table available for other organisms of industrial interest using CUTE.
