Team:Minnesota/Results
Team:Minnesota
#Minnesota 2018
RESULTS:
Figure 1. Gel electrophoresis results for BamHI digested Mer-GlnA-GFP(Tn501) plasmid.
In figure 1, the bands on lane No. 2, 3, 6, 7, 12 represented successful plasmid construction. Comparing to the ladder, our bands have a size of roughly 7.7kb, which is the length that we are looking for.
Figure 2. Gel electrophoresis results for BamHI digested Mer-GlnA-GFP(pDu) plasmid.
In figure 2, the lanes are counted in a right to left, up to down fashion.
Lanes number 4, 13 and 14 show bands at roughly 7.7 kb according to our ladder separation.
We also expect the length of our digested product to be at this length, so we can conclude that the plasmid construct is successful.
Figure 3: Gel Electrophoresis result for Mer-GlnA-GFP(pDu) plasmid
The length of Mer-GlnA-GFP(Tn501) plasmid should be 7.7kb. Figure 3 shows that the length of plasmids we constructed are 7.7kb.
We concluded that the Mer-GlnA-GFP(pDu) plasmids were successfully constructed.
The length of our LacI-GlnA-GFP plasmid should be 5.5kb. Figure 4 shows that samples in well 1, 2, 6, 10, 12 have the correct length.
We concluded those LacI-GlnA-GFP plasmids were successfully constructed.
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