Team:Kyoto/Model

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1)Abstract

We have to optimize combination of recombinant genes and amount of yeast when we apply our salt up taking system to real environment. But these are nontrivial because of cytosolic sodium ions’ inhibition of growth or alteration of intracellular Na⁺’s flux. We tried to make mathematical model that gives initial value corresponding to objective amount of salt decrease. We described yeast cell’s kinetics of salt absorbance. Then, using this model, we looked for best combination of recombinant genes. Through experiments, we calculated yeast’s behavior when the yeast has single protein in this project. We decided kinetic parameter of transporter with our model. Finally, we simulated possible pattern of recombinant yeast in our project.