Team:BioIQS-Barcelona/Results

Project | Results

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Achievements of our project

1.

Computationally demonstrated that primer binding regions are perfectly conserved or slightly different for both DQ2 and DQ8 celiac haplotypes separately. This means that either the same primer can be used for amplifying all genotypes or that a specific DQ2- or DQ8-primer has to be used.

2.

Successfully designed a simple and cheap way to extract the extracellular domain of both alpha and beta chains of the HLA-DQ protein from the human genome. This would make the extraction of all the HLA-DQ variants possible with only one protocol.

3.

Thoroughly created different synthetic constructs to ensure the correct expression of the HLA-DQ in a bacterial or yeast host. This would enable us to find the best way to use them in a sensor.

4.

Correctly cloned one of the constructs into a desired expression vector and prepared it for further expression experiments. Cloning is a tedious work and not always results as one would expect so this is an important achievement!

5.

Carefully built a theoretical model to illustrate the essential mechanism of the sensor. Modelling has become one of the most important jobs in this project and have allowed us to better understand the role of the sensor.