Team:Vilnius-Lithuania/Basic
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Basic Parts
Separate Soldiers
Basic parts, that we created fall into three categories: coding, regulatory and RNA. Coding parts consist of proteins, that assemble into lipid bilayer. Regulatory parts ensure that protein synthesis starts at desired temperature. RNA is used as a modification platform to produce membrane associated ribosomes which help with protein integration into membrane.
Basic Parts
|
Part Number |
Type |
Name |
Brief description |
Length, nt |
|
Coding |
BamB |
Beta-barrel assembly protein B (BamB) |
1261 |
|
|
Coding |
BamA |
Beta-barrel assembly protein A (BamA) |
2520 |
|
|
Coding |
IgA |
Immunoglobulin protease A (IgA) beta-domain from Neisseria gonorrhoeae |
1398 |
|
|
Coding |
scFv_antiVLY |
Single chain variable fragment antibody anti-vaginolysin (scFv_antiVGY) |
733 |
|
|
Coding |
GFP Nanobody |
Camelid antibody against green fluorescent protein (GFP Nanobody) |
352 |
|
|
Coding |
Mistic (Mstx) |
Membrane-integrating protein Mistic (MstX) from Bacillus subtilis |
330 |
|
|
RNA |
gRNA for ribosome protein L12 |
Guide RNA for modification of ribosome protein L12 using CRISPR-Cas9 system |
174 |
|
|
RNA |
gRNA for ribosome protein L23 |
Guide RNA for modification of ribosome protein L23 using CRISPR-Cas9 system |
174 |
|
|
RNA |
gRNA for ribosome protein L24 |
Guide RNA for modification of ribosome protein L24 using CRISPR-Cas9 system |
174 |
|
|
Regulatory |
RNA thermoswitch1 (GJ2) |
Temperature-sensitive non-coding RNA 1 |
39 |
|
|
Regulatory |
RNA thermoswitch2 (GJ3) |
Temperature-sensitive non-coding RNA 2 |
38 |
|
|
Regulatory |
RNA thermoswitch3 (GJ6) |
Temperature-sensitive non-coding RNA 3 |
53 |
|
|
Regulatory |
RNA thermoswitch4 (GJ9) |
Temperature-sensitive non-coding RNA 4 |
32 |
|
|
Regulatory |
RNA thermoswitch5 (GJ10) |
Temperature-sensitive non-coding RNA 5 |
33 |
|
|
Coding |
BamC |
Beta-barrel assembly protein C (BamC) |
1057 |
|
|
Regulatory |
RNA thermoswitch 6 (Sw2) |
Temperature-sensitive non-coding RNA 6 |
33 |
|
|
Regulatory |
RNA thermoswitch 7 (Sw3) |
Temperature-sensitive non-coding RNA 7 |
33 |
|
|
Regulatory |
RNA thermoswitch 8 (Sw6) |
Temperature-sensitive non-coding RNA 8 |
33 |
|
|
Regulatory |
RNA thermoswitch 9 (Sw7) |
Temperature-sensitive non-coding RNA 9 |
33 |
|
|
Regulatory |
RNA thermoswitch 10 (Sw9) |
Temperature-sensitive non-coding RNA 10 |
33 |
|
|
Regulatory |
RNA thermoswitch 11 (Sw11) |
Temperature-sensitive non-coding RNA 11 |
33 |
|
|
Coding |
BamD |
Beta-barrel assembly protein D (BamD) |
757 |
|
|
Coding |
BamE |
Beta-barrel assembly protein E (BamE) |
448 |
|
|
Coding |
SurA |
Survival protein A (SurA) |
1374 |
|
|
Coding |
OmpA full |
Full length outer transmembrane protein A (OmpA) |
1041 |
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