Template:Vilnius-Lithuania/InnerPageJS
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abstract: "Liposomes are at the heart of SynDrop. Unlike cells, these adjustable synthetic containers can be constructed only from well-defined parts and elements that are necessary for a particular experiment, leaving no place for background noise, cross-talk and inconsistencies. Encapsulating transcriptional and translational systems within the liposomes transforms them into effective bioreactors with applications only limited by our own imagination. With the help of microfluidics, extensive modeling and self-made custom software we are able to produce cell-sized, monodisperse and biocompatible liposomes with nearly maximal encapsulation efficiency. With these qualities they are perfectly suitable for membrane protein synthesis, folding and integration into the membrane.",
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abstract: "Self-inserting α-helical protein Mistic (MstX) is unique due to its uncommonly hydrophilic surface. Recently it has found a novel application as a fusion tag supporting the recombinant production and bilayer insertion of other membrane proteins. When fused to the N-terminus of integral membrane proteins, MstX enables the cargo proteins to fold into their native conformations in the membrane, thus yielding a high-level expression.",
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abstract: "Since liposomes can maintain the genotype-phenotype link, they could potentially be used as tool to perform molecular evolution. To elaborate this perspective, we constructed a bunch of novel and effective membrane protein fusions that could be used to display various epitopes to the surface of liposomes.",
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abstract: "Mathematical modeling is an approach to understand the processes that alter behaviors of different systems. Synthetic biology is inescapably involved in modeling as it requires deeper knowledge of how distinct processes form one indispensable system in order to create one. With this knowledge, one can manipulate parameters and design unique system with desired features. During creation of our system we hypothesized that adding target membrane protein messenger RNA (mRNA) would ensure that this protein folds and inserts into liposome membrane correctly as well as enhances other membrane protein expression. Mathematical model for necessity of mRNA addition to IVTT system was generated to compare results primarily across different starting volumes of target protein mRNA. It was done in order to quantify the effectiveness of an initial addition of mRNA ensuring fast expression of target membrane protein for creating more efficient cell-free system for membrane protein research.",
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abstract: "Together with colleagues from Vienna University we have compiled a simple in silico translation-initiation potential model to quantify the likelihood of translation of a mRNA bearing thermoswitch sequence at varying temperatures. The model resulted in a great number of de novo synthetic thermoswitches and provided theoretical evidence of their behaviour thus allowing us to choose and apply only the best-performing RNA thermometers for the SynDrop system.",
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abstract: "Analyzing means to reduce the workload and time consumption while working on our project and wishing to extend our collaboration with current Lithuanian IT leaders, we organized an international life sciences BioHackathon. The successful event resulted in many wet-lab-applicable projects and most importantly, it provided the base for our software tool LipoVision. This software is currently being tested in our University’s research centre, Microfluidics lab, where LipoVision promises to become a daily helper in all liposome synthesis-related experiments.",
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abstract: "Encouraged by the rapid Life Sciences development in Lithuania and our team’s previous achievements, we decided that it was meaningful to share our experience with the younger generation. Visiting schools, inviting pupils to visit Vilnius University Life Sciences Center and introducing SynDrop to them helped us not only to reveal but also clarify our own attitude towards synthetic biology. The discussion that we have organised during the DNA Day’s celebration has become a great inspiration to search for a creative approach to implement our project’s idea and to make it more public-friendly.",
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