Team:NTNU Trondheim/Notebook

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Notebook

• Week

  25

  Note title

  • Jun

    18

    Test

    Test.
  • Jun

    19

    Note title

    Lorem ipsum dolor sit amet.
  • Jun

    20

    Our first day in the laboratory!

    Goal: Incubate the Escherichia coli (E. coli) samples which carry pgRNA and pdCas9 on agar plates with antibiotics.
    Preparation of LA- medium
    LA- medium (1.5%) were made after the following recipe, and autoclaved for 20min at 121°C.

    • 1L Distilled water
    • 5g Yeast extract
    • 10g Tryptone
    • 5g NaCl
    • 15g Agar

    
    Agar plates with antibiotics
    Two different agar plates were made – one type contains ampicillin (AMP) while the other type contains chloramphenicol (CM), and were prepared after the following recipe:
    LA- medium with AMP:

    • 0.5L LA-medium
    • 0.5mL AMP (50mg/mL)

    
    LA-medium with CM:

    • 0.5L LA-medium
    • 0.5mL CM (35mg/mL)

    The LA-media with antibiotic were poured on petri dishes.
  • Jun

    21

    Inoculation of E.coli from agar plates to LB-medium

    Goal: Inoculate a colony from each agar plate which were prepared from the previous day, in LB- medium with antibiotics.
    
    Procedure
    One colony of pgRNA- and pdCas9- bacteria were inoculated in LB-media (25mL) with AMP (25μL) and CM (25μL), respectively. The cell cultures were incubated at 37℃ in a shaking incubator at 204rpm.
    
  • Jun

    22

    Isolation and verification of pgRNA and pdCas9

    Test
• Week

  26

  Note title

  • Jun

    25

    Note title

    Lorem ipsum dolor sit amet.
  • Jun

    26

    Note title

    Lorem ipsum dolor sit amet.
  • Jun

    27

    Note title

    Lorem ipsum dolor sit amet.
  • Jun

    28

    Note title

    Lorem ipsum dolor sit amet.
  • Jun

    29

    Note title

    Lorem ipsum dolor sit amet.
• Week

  27

  Note title

  • Jul

    1

    Note title
  • Jul

    2

    InterLabs Study

    Calibration 1 and 2 for the InterLabs Study.
  • Jul

    3

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    4

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    5

    Note title

    Lorem ipsum dolor sit amet.
• Week

  28

  Note title

  • Jul

    9

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    10

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    11

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    12

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    13

    Note title

    Lorem ipsum dolor sit amet.
• Week

  29

  Note title

  • Jul

    16

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    17

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    18

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    19

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    20

    Note title

    Lorem ipsum dolor sit amet.
• Week

  30

  Note title

  • Jul

    23

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    24

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    25

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    26

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    27

    Note title

    Lorem ipsum dolor sit amet.
• Week

  31

  Note title

  • Jul

    30

    Note title

    Lorem ipsum dolor sit amet.
  • Jul

    31

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    1

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    2

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    3

    Note title

    Lorem ipsum dolor sit amet.
• Week

  32

  Note title

  • Aug

    6

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    7

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    8

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    9

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    10

    Note title

    Lorem ipsum dolor sit amet.
• Week

  33

  Note title

  • Aug

    13

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    14

    Note title

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  • Aug

    15

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    16

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    17

    Note title

    Lorem ipsum dolor sit amet.
• Week

  34

  Note title

  • Aug

    20

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    21

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    22

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    23

    Note title

    Lorem ipsum dolor sit amet.
  • Aug

    24

    Note title

    Lorem ipsum dolor sit amet.