Team:NTNU Trondheim/Notebook

• Team
  Team Members Collaborations
• Project
  Description Design Model Results Demonstrate
• Lab
  Lab Journal Protocols Measurement Safety
• Parts
  Parts Overview New Part
• InterLab
• Human Practices
  Integrated Human Practices Education and Engangement
• Acknowledgements
  Sponsors Attributions
• Awards

Notebook

• Week

  25

  Getting started

  • Jun

    18

    No lab

    Test.
  • Jun

    19

    No lab

    Contacted some potential sponsors and continued our search for new ones. The project description was entered on our wiki page. We had a quick lab tour guided by our instructor Nikolay.
  • Jun

    20

    Our first day in the laboratory!

    Contacted some sponsors, read articles related to our project and started on the script for the modelling of our project.
    We finally started working in the laboratory. We made LA-medium and plated bacteria carrying pgRNA and pdCas9 on agar plates with ampicillin and chloramphenicol, respectively. More details about the lab procedures throughout the project are given in our lab journal.
  • Jun

    21

    Inoculation of E.coli

    Pure cultures were made by picking and inoculating colonies grown overnight on agar plates with antibiotics as selection agents.
  • Jun

    22

    Isolation and verification of pgRNA and pdCas9

    Verified that the bacteria in our pure cultures had been correctly transformed with the pgRNA and the pdCas9. This was done by plasmid isolation (Miniprep), restriction cutting with appropriate enzymes and agarose gel electrophoresis (test digest).
• Week

  26

  Note title

  • Jun

    25

    Note title

    Inserted pgRNA and pdCas9 into competent E. coli DH5α by heat shock transformation, and aseptically spread plated the transformation mixes on separate agar plates with ampicillin and chloramphenicol, respectively. We also started preparing for the InterLabs Study.
  • Jun

    26

    Note title

    Pure cultures were made by Inoculating colonies from the agar plates prepared yesterday. We started to work with the InterLab Study: Calibration curve 1 and 2 were made.
  • Jun

    27

    Note title

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  • Jun

    28

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  • Jun

    29

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• Week

  27

  Note title

  • Jul

    1

    Note title
  • Jul

    2

    InterLabs Study

    Calibration 1 and 2 for the InterLabs Study.
  • Jul

    3

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  • Jul

    4

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  • Jul

    5

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• Week

  28

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  • Jul

    9

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  • Jul

    10

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  • Jul

    11

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  • Jul

    12

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  • Jul

    13

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• Week

  29

  Note title

  • Jul

    16

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  • Jul

    17

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  • Jul

    18

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  • Jul

    19

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  • Jul

    20

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• Week

  30

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  • Jul

    23

    Note title

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  • Jul

    24

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  • Jul

    25

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  • Jul

    26

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  • Jul

    27

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• Week

  31

  Note title

  • Jul

    30

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  • Jul

    31

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  • Aug

    1

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  • Aug

    2

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  • Aug

    3

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• Week

  32

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  • Aug

    6

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  • Aug

    7

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  • Aug

    8

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  • Aug

    9

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  • Aug

    10

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• Week

  33

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  • Aug

    13

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  • Aug

    14

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  • Aug

    15

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  • Aug

    16

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  • Aug

    17

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• Week

  34

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  • Aug

    20

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  • Aug

    21

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  • Aug

    22

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  • Aug

    23

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  • Aug

    24

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