Team:Rotterdam HR/Notebook
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June
Tom van Dijk | Mike Vrieswijk
The first draft of the architecture design for the temperature controller was made.
01
Architecture design [TC]
18-6-2018
Tom van Dijk | Mike Vrieswijk
Today a few changes have been made to the architecture design of the temperature controller. The choice was made to use a premade power supply as opposed to a self-made one, because this was considering the time left to finish this device the best choice. A few alterations were made to make the architecture design better readable.
02
Architecture design [TC]
24-6-2018
Tom van Dijk | Mike Vrieswijk
A 3d drawing was made for the casing that will hold the temperature controller. The first prototype was printed, but was determent to be inadequate due to the lack of enough space for the cooler block.
01
First prototype [TC]
25-6-2018
Tom van Dijk | Mike Vrieswijk
In the drawing space was added to fit the cooler block for the temperature controller. Slits were added to the drawing for the nuts to slide into. The altered drawing was printed. The newly printed parts, cooler block, fan and Peltier element were assembled. Everything fit together.
02
Second prototype [TC]
26-6-2018
Tom van Dijk | Mike Vrieswijk
A short test run was done with the newly assembled cooler. The test concluded that the temperature controller could cool a water droplet to the point of freezing and heat it back up to its boiling point.
03
Testrun [TC]
27-6-2018
July
Tom van Dijk | Mike Vrieswijk
The choice to use a computer PSU as a power supply was made. The choice was made on the grounds, that computer PSU have a standard 12, 5 and 3.3V output. This is beneficial because these are the voltages necessary for the selected components.
01
Draft decision
2-7-2018
Tom van Dijk | Mike Vrieswijk
Changes were made to the architecture design of the temperature controller. Different voltage regulators were selected to fit the newly chosen power supply. To better readability of the architecture design a legend was added.
03
Architecture design [TC]
5-7-2018
Tom van Dijk | Mike Vrieswijk
Custom footprints were made for multiple components and added to the parts library in the PCB design software.
01
Electrical circuit design [TC]
8-7-2018
Tom van Dijk | Mike Vrieswijk
The provisional electronic circuit design for the temperature controller was made.
02
Electrical circuit design [TC]
9-7-2018
Elise Grootscholten | Loraine Nelson | Paul Reusink
To have some basic biobricks ready, we stocked them by doing a plasmid isolation and freeze the DNA.
01
Stock up
11-7-2018
Elise Grootscholten | Loraine Nelson | Paul Reusink
To start on a basic construct we did a digestion and ligation with the parts we isolated earlier. To prevent original constructs we did a defosphorylation after the digestion. We controlled the digestion by doing a gelelktroforeses.
01
Basic parts
11-7-2018
Tom van Dijk | Mike Vrieswijk
The electronic circuit design for the temperature controller was finished. Measuring point were added to the electronic circuit design.
03
Electrical circuit design [TC]
11-7-2018
Tom van Dijk | Randall de Waard
We continued with the ligated DNA by transformating it to Neb10Beta. This was plated onto agarplates and incubated for 1 day.
02
Basic parts
12-7-2018
Tom van Dijk | Mike Vrieswijk
Started working on the board layout for the PCB of the temperature controller.
01
Board layout [TC]
12-7-2018
Elise Grootscholten | Randall de Waard
The results of 7/12/2018 were collected, in action of this we decided to plate some ligated and transferred DNA from 7/12/2018 again. This we did because there wasn't a clear grow of colonies on the plates.
03
Basic parts
13-7-2018
Elise Grootscholten | Randall de Waard
We tried to let bacteria grow on paper, therefore we used our own buisnesscards. We let the buisnesscards absorb some LB-medium and put them inside petridishes.
01
Blue White screening on paper
13-7-2018
Tom van Dijk | Mike Vrieswijk
Further work was done to the board layout for the temperature controller.
02
Board layout [TC]
13-7-2018
Tom van Dijk | Mike Vrieswijk
Work has been done on the board layout for the temperature controller. The board layout is now finished.
03
Board layout [TC]
15-7-2018
Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard
To know if the ligation was succesfull we will controll the DNA, therefor we ented the bacteria's with DNA in LB-medium to grow overnight. We also plated the used bacteria's onto new plates to use for further experiments.
04
Basic parts
16-7-2018
Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Randall de Waard
We did a blue white screening on our buisnesscards to see if bacteria had grew on them. We transferred the buisnesscards to new petrydishes and added ITPG and X-gal to the petrydishes.
02
Blue White screening on paper
16-7-2018
Rens Boeser | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard
The resulst of 7/16/2018 showed that the DNA didnt ligate as planned. To be able to digest enough DNA we did a plasmid isolation again. We digested this DNA and froze it for further use.
05
Basic parts
17-7-2018
Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Mike Vrieswijk | Randall de Waard
We did another round of plasmid isolation for stocking.
02
Stock up
18-7-2018
Dustin van der Meulen | Mei Ju Goemans| Randall de Waard
We digested all DNA we have in stock to test for our basic construct. We tested the DNA by doing a gelelktroforeses.
06
Basic parts
19-7-2018
Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard
New biobricks, used to make an ATP sensor, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day.
01
ATP sensor
24-7-2018
Rens Boeser | Mei Ju Goemans | Elise Grootscholten | Dustin van der Meulen | Loraine Nelson | Suzanne Romeijn | Randall de Waard
New biobricks, which produces gasses, were transformed into NEB10Beta. The culture was than plated onto agar and incubated for 1 day.
01