Team:NUS Singapore-Sci/Wetlab/Interlab

• Project
  • Project Overview
  • Reporter System
    • Reporter Overview
    • Dual Reporter - Demonstration
    • Dual Reporter - Improvement
    • GLB1 Reporter
  • Editor System
    • Editor Overview
    • Editor Results
  • Modelling
    • Modelling Overview
    • Kalman Filter Ensemble
    • Molecular Dynamics
    • Enzyme Kinetics
    • gRNA Design
  • InterLab
• Human Practice
  • Overview of Human Practice
  • Public Engagement
  • #CasAsks
    • #CasAsks: Survey
    • #CasAsks: Interview
  • #CasTalks
  • #CasTeaches
  • #CasWrites
    • #CasWrites: Discussion
    • #CasWrites: Collaboration
    • #CasWrites: BioEthics
• Collaborations
• Notebook
  • Reporter Logbooks
    • Dual Reporter Logbook
    • GLB1 Reporter Logbook
  • Editor Logbook
  • Primers and Protocols
• People
  • Team
  • Attributions
• Admin
  • Safety
  • Medals
  • Parts

This year, our team took part in the 2018 InterLab Study, which aims to standardise the fluorescence levels of bacteria transformed with different devices conferring it fluorescence. This is accomplished through the use of a plate reader. The plate reader we used was Synergy H1 Hybrid Multi-Mode Reader (courtesy of SynCTI?), which could read both absorbance and fluorescence.

For all the following measurements, we did not use pathlength correction, and kept the temperature setting to room temperature (20-25°C). The bandpass width is fixed at 16 nm. Bottom optics are used.

Before cell measurements were taken, three different calibration protocols were done.

1) OD600 Reference Point - LUDOX Protocol

LUDOX CL-X (45% colloidal silica suspension) was used to convert the Absorbance at 600 nm as obtained from the plate reader into a comparable OD600 measurement as obtained from a spectrophotometer. Here, pathlength correction is especially important to be switched off so as to not provide correction for the volume of sample in the 96-well plate.