Team:RHIT/Parts




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Basic Part
Composite Part
Collection

Plasmid 1 Parts

  • BBa_K2716000 - Optimized Double-Mutant PETase

    PETase is an aromatic polyesterase that breaks PET into MHET, as well as some terephthalic acid and BHET. The double-mutated optimized PETase contains a S238F/W159H mutation, allowing for a more efficient enzyme.

  • BBa_K2716002 - MHETase

    MHETase breaks MHET into terephthalic acid and ethylene glycol.

  • BBa_K2716100 - Complete Plasmid 1

    This composite part contains a promoter, a ribosomal binding site, the double-mutated optimized PETase, a secretion tag (pelB), MHETase, and a terminator. PETase is an aromatic polyesterase that breaks PET into MHET, as well as some terephthalic acid and BHET. The double-mutated optimized PETase contains a S238F/W159H mutation, allowing for a more efficient enzyme. PelB is a secretion tag that will allow the PETase enzyme to be secreted outside of the cell. This increases the efficiency of the PET breakdown. MHETase breaks MHET into terephthalic acid and ethylene glycol. The second secretion tag is added so that the MHETase enzyme will also be secreted.

  • BBa_K2716101 - Promoter+RBS+pelB+PETase

    This composite part contains a promoter, a ribosomal binding site, the double-mutated optimized PETase, and a secretion tag (pelB). PETase is an aromatic polyesterase that breaks PET into MHET, as well as some terephthalic acid and BHET. The double-mutated optimized PETase contains a S238F/W159H mutation, allowing for a more efficient enzyme. PelB is a secretion tag that will allow the PETase enzyme to be secreted outside of the cell. This increases the efficiency of the PET breakdown.

What information do I need to start putting my parts on the Registry?

The information needed to initially create a part on the Registry is:

  • Part Name
  • Part type
  • Creator
  • Sequence
  • Short Description (60 characters on what the DNA does)
  • Long Description (Longer description of what the DNA does)
  • Design considerations

We encourage you to put up much more information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page.

<groupparts>iGEM18 RHIT</groupparts>