Team:RHIT/Parts
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Plasmid 1 Parts
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BBa_K2716000 - Optimized Double-Mutant PETase
PETase is an aromatic polyesterase that breaks PET into MHET, as well as some terephthalic acid and BHET. The double-mutated optimized PETase contains a S238F/W159H mutation, allowing for a more efficient enzyme. -
BBa_K2716002 - MHETase
MHETase breaks MHET into terephthalic acid and ethylene glycol.
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BBa_K2716100 - Complete Plasmid 1
This composite part contains a promoter, a ribosomal binding site, the double-mutated optimized PETase, a secretion tag (pelB), MHETase, and a terminator. PETase is an aromatic polyesterase that breaks PET into MHET, as well as some terephthalic acid and BHET. The double-mutated optimized PETase contains a S238F/W159H mutation, allowing for a more efficient enzyme. PelB is a secretion tag that will allow the PETase enzyme to be secreted outside of the cell. This increases the efficiency of the PET breakdown. MHETase breaks MHET into terephthalic acid and ethylene glycol. The second secretion tag is added so that the MHETase enzyme will also be secreted. -
BBa_K2716101 - Promoter+RBS+pelB+PETase
This composite part contains a promoter, a ribosomal binding site, the double-mutated optimized PETase, and a secretion tag (pelB). PETase is an aromatic polyesterase that breaks PET into MHET, as well as some terephthalic acid and BHET. The double-mutated optimized PETase contains a S238F/W159H mutation, allowing for a more efficient enzyme. PelB is a secretion tag that will allow the PETase enzyme to be secreted outside of the cell. This increases the efficiency of the PET breakdown.
<groupparts>iGEM18 RHIT</groupparts>