! function (t, e) {
"object" == typeof exports && "undefined" != typeof module ? e(exports, require("jquery"), require("popper.js")) : "function" == typeof define && define.amd ? define(["exports", "jquery", "popper.js"], e) : e(t.bootstrap = {}, t.jQuery, t.Popper)
}(this, function (t, e, c) {
"use strict";
function i(t, e) { for (var n = 0; n < e.length; n++) { var i = e[n]; i.enumerable = i.enumerable || !1, i.configurable = !0, "value" in i && (i.writable = !0), Object.defineProperty(t, i.key, i) } }
function o(t, e, n) { return e && i(t.prototype, e), n && i(t, n), t }
function h(r) { for (var t = 1; t < arguments.length; t++) { var s = null != arguments[t] ? arguments[t] : {}, e = Object.keys(s); "function" == typeof Object.getOwnPropertySymbols && (e = e.concat(Object.getOwnPropertySymbols(s).filter(function (t) { return Object.getOwnPropertyDescriptor(s, t).enumerable }))), e.forEach(function (t) { var e, n, i; e = r, i = s[n = t], n in e ? Object.defineProperty(e, n, { value: i, enumerable: !0, configurable: !0, writable: !0 }) : e[n] = i }) } return r } e = e && e.hasOwnProperty("default") ? e.default : e, c = c && c.hasOwnProperty("default") ? c.default : c; var r, n, s, a, l, u, f, d, _, g, m, p, v, E, y, T, C, I, A, D, b, S, w, N, O, k, P, L, j, R, H, W, M, x, U, K, F, V, Q, B, Y, G, q, z, X, J, Z, $, tt, et, nt, it, rt, st, ot, at, lt, ht, ct, ut, ft, dt, _t, gt, mt, pt, vt, Et, yt, Tt, Ct, It, At, Dt, bt, St, wt, Nt, Ot, kt, Pt, Lt, jt, Rt, Ht, Wt, Mt, xt, Ut, Kt, Ft, Vt, Qt, Bt, Yt, Gt, qt, zt, Xt, Jt, Zt, $t, te, ee, ne, ie, re, se, oe, ae, le, he, ce, ue, fe, de, _e, ge, me, pe, ve, Ee, ye, Te, Ce, Ie, Ae, De, be, Se, we, Ne, Oe, ke, Pe, Le, je, Re, He, We, Me, xe, Ue, Ke, Fe, Ve, Qe, Be, Ye, Ge, qe, ze, Xe, Je, Ze, $e, tn, en, nn, rn, sn, on, an, ln, hn, cn, un, fn, dn, _n, gn, mn, pn, vn, En, yn, Tn, Cn = function (i) { var e = "transitionend";
function t(t) { var e = this, n = !1; return i(this).one(l.TRANSITION_END, function () { n = !0 }), setTimeout(function () { n || l.triggerTransitionEnd(e) }, t), this } var l = { TRANSITION_END: "bsTransitionEnd", getUID: function (t) { for (; t += ~~(1e6 * Math.random()), document.getElementById(t);); return t }, getSelectorFromElement: function (t) { var e = t.getAttribute("data-target"); e && "#" !== e || (e = t.getAttribute("href") || ""); try { return 0 < i(document).find(e).length ? e : null } catch (t) { return null } }, getTransitionDurationFromElement: function (t) { if (!t) return 0; var e = i(t).css("transition-duration"); return parseFloat(e) ? (e = e.split(",")[0], 1e3 * parseFloat(e)) : 0 }, reflow: function (t) { return t.offsetHeight }, triggerTransitionEnd: function (t) { i(t).trigger(e) }, supportsTransitionEnd: function () { return Boolean(e) }, isElement: function (t) { return (t[0] || t).nodeType }, typeCheckConfig: function (t, e, n) { for (var i in n) if (Object.prototype.hasOwnProperty.call(n, i)) { var r = n[i], s = e[i], o = s && l.isElement(s) ? "element" : (a = s, {}.toString.call(a).match(/\s([a-z]+)/i)[1].toLowerCase()); if (!new RegExp(r).test(o)) throw new Error(t.toUpperCase() + ': Option "' + i + '" provided type "' + o + '" but expected type "' + r + '".') } var a } }; return i.fn.emulateTransitionEnd = t, i.event.special[l.TRANSITION_END] = { bindType: e, delegateType: e, handle: function (t) { if (i(t.target).is(this)) return t.handleObj.handler.apply(this, arguments) } }, l }(e), In = (n = "alert", a = "." + (s = "bs.alert"), l = (r = e).fn[n], u = { CLOSE: "close" + a, CLOSED: "closed" + a, CLICK_DATA_API: "click" + a + ".data-api" }, f = "alert", d = "fade", _ = "show", g = function () { function i(t) { this._element = t } var t = i.prototype; return t.close = function (t) { t = t || this._element; var e = this._getRootElement(t); this._triggerCloseEvent(e).isDefaultPrevented() || this._removeElement(e) }, t.dispose = function () { r.removeData(this._element, s), this._element = null }, t._getRootElement = function (t) { var e = Cn.getSelectorFromElement(t), n = !1; return e && (n = r(e)[0]), n || (n = r(t).closest("." + f)[0]), n }, t._triggerCloseEvent = function (t) { var e = r.Event(u.CLOSE); return r(t).trigger(e), e }, t._removeElement = function (e) { var n = this; if (r(e).removeClass(_), r(e).hasClass(d)) { var t = Cn.getTransitionDurationFromElement(e); r(e).one(Cn.TRANSITION_END, function (t) { return n._destroyElement(e, t) }).emulateTransitionEnd(t) } else this._destroyElement(e) }, t._destroyElement = function (t) { r(t).detach().trigger(u.CLOSED).remove() }, i._jQueryInterface = function (n) { return this.each(function () { var t = r(this), e = t.data(s); e || (e = new i(this), t.data(s, e)), "close" === n && e[n](this) }) }, i._handleDismiss = function (e) { return function (t) { t && t.preventDefault(), e.close(this) } }, o(i, null, [{ key: "VERSION", get: function () { return "4.1.0" } }]), i }(), r(document).on(u.CLICK_DATA_API, '[data-dismiss="alert"]', g._handleDismiss(new g)), r.fn[n] = g._jQueryInterface, r.fn[n].Constructor = g, r.fn[n].noConflict = function () { return r.fn[n] = l, g._jQueryInterface }, g), An = (p = "button", E = "." + (v = "bs.button"), y = ".data-api", T = (m = e).fn[p], C = "active", I = "btn", D = '[data-toggle^="button"]', b = '[data-toggle="buttons"]', S = "input", w = ".active", N = ".btn", O = { CLICK_DATA_API: "click" + E + y, FOCUS_BLUR_DATA_API: (A = "focus") + E + y + " blur" + E + y }, k = function () { function n(t) { this._element = t } var t = n.prototype; return t.toggle = function () { var t = !0, e = !0, n = m(this._element).closest(b)[0]; if (n) { var i = m(this._element).find(S)[0]; if (i) { if ("radio" === i.type) if (i.checked && m(this._element).hasClass(C)) t = !1; else { var r = m(n).find(w)[0]; r && m(r).removeClass(C) } if (t) { if (i.hasAttribute("disabled") || n.hasAttribute("disabled") || i.classList.contains("disabled") || n.classList.contains("disabled")) return; i.checked = !m(this._element).hasClass(C), m(i).trigger("change") } i.focus(), e = !1 } } e && this._element.setAttribute("aria-pressed", !m(this._element).hasClass(C)), t && m(this._element).toggleClass(C) }, t.dispose = function () { m.removeData(this._element, v), this._element = null }, n._jQueryInterface = function (e) { return this.each(function () { var t = m(this).data(v); t || (t = new n(this), m(this).data(v, t)), "toggle" === e && t[e]() }) }, o(n, null, [{ key: "VERSION", get: function () { return "4.1.0" } }]), n }(), m(document).on(O.CLICK_DATA_API, D, function (t) { t.preventDefault(); var e = t.target; m(e).hasClass(I) || (e = m(e).closest(N)), k._jQueryInterface.call(m(e), "toggle") }).on(O.FOCUS_BLUR_DATA_API, D, function (t) { var e = m(t.target).closest(N)[0]; m(e).toggleClass(A, /^focus(in)?$/.test(t.type)) }), m.fn[p] = k._jQueryInterface, m.fn[p].Constructor = k, m.fn[p].noConflict = function () { return m.fn[p] = T, k._jQueryInterface }, k), Dn = (L = "carousel", R = "." + (j = "bs.carousel"), H = ".data-api", W = (P = e).fn[L], M = { interval: 5e3, keyboard: !0, slide: !1, pause: "hover", wrap: !0 }, x = { interval: "(number|boolean)", keyboard: "boolean", slide: "(boolean|string)", pause: "(string|boolean)", wrap: "boolean" }, U = "next", K = "prev", F = "left", V = "right", Q = { SLIDE: "slide" + R, SLID: "slid" + R, KEYDOWN: "keydown" + R, MOUSEENTER: "mouseenter" + R, MOUSELEAVE: "mouseleave" + R, TOUCHEND: "touchend" + R, LOAD_DATA_API: "load" + R + H, CLICK_DATA_API: "click" + R + H }, B = "carousel", Y = "active", G = "slide", q = "carousel-item-right", z = "carousel-item-left", X = "carousel-item-next", J = "carousel-item-prev", Z = { ACTIVE: ".active", ACTIVE_ITEM: ".active.carousel-item", ITEM: ".carousel-item", NEXT_PREV: ".carousel-item-next, .carousel-item-prev", INDICATORS: ".carousel-indicators", DATA_SLIDE: "[data-slide], [data-slide-to]", DATA_RIDE: '[data-ride="carousel"]' }, $ = function () { function s(t, e) { this._items = null, this._interval = null, this._activeElement = null, this._isPaused = !1, this._isSliding = !1, this.touchTimeout = null, this._config = this._getConfig(e), this._element = P(t)[0], this._indicatorsElement = P(this._element).find(Z.INDICATORS)[0], this._addEventListeners() } var t = s.prototype; return t.next = function () { this._isSliding || this._slide(U) }, t.nextWhenVisible = function () { !document.hidden && P(this._element).is(":visible") && "hidden" !== P(this._element).css("visibility") && this.next() }, t.prev = function () { this._isSliding || this._slide(K) }, t.pause = function (t) { t || (this._isPaused = !0), P(this._element).find(Z.NEXT_PREV)[0] && (Cn.triggerTransitionEnd(this._element), this.cycle(!0)), clearInterval(this._interval), this._interval = null }, t.cycle = function (t) { t || (this._isPaused = !1), this._interval && (clearInterval(this._interval), this._interval = null), this._config.interval && !this._isPaused && (this._interval = setInterval((document.visibilityState ? this.nextWhenVisible : this.next).bind(this), this._config.interval)) }, t.to = function (t) { var e = this; this._activeElement = P(this._element).find(Z.ACTIVE_ITEM)[0]; var n = this._getItemIndex(this._activeElement); if (!(t > this._items.length - 1 || t < 0)) if (this._isSliding) P(this._element).one(Q.SLID, function () { return e.to(t) }); else { if (n === t) return this.pause(), void this.cycle(); var i = n < t ? U : K; this._slide(i, this._items[t]) } }, t.dispose = function () { P(this._element).off(R), P.removeData(this._element, j), this._items = null, this._config = null, this._element = null, this._interval = null, this._isPaused = null, this._isSliding = null, this._activeElement = null, this._indicatorsElement = null }, t._getConfig = function (t) { return t = h({}, M, t), Cn.typeCheckConfig(L, t, x), t }, t._addEventListeners = function () { var e = this; this._config.keyboard && P(this._element).on(Q.KEYDOWN, function (t) { return e._keydown(t) }), "hover" === this._config.pause && (P(this._element).on(Q.MOUSEENTER, function (t) { return e.pause(t) }).on(Q.MOUSELEAVE, function (t) { return e.cycle(t) }), "ontouchstart" in document.documentElement && P(this._element).on(Q.TOUCHEND, function () { e.pause(), e.touchTimeout && clearTimeout(e.touchTimeout), e.touchTimeout = setTimeout(function (t) { return e.cycle(t) }, 500 + e._config.interval) })) }, t._keydown = function (t) { if (!/input|textarea/i.test(t.target.tagName)) switch (t.which) { case 37: t.preventDefault(), this.prev(); break; case 39: t.preventDefault(), this.next() } }, t._getItemIndex = function (t) { return this._items = P.makeArray(P(t).parent().find(Z.ITEM)), this._items.indexOf(t) }, t._getItemByDirection = function (t, e) { var n = t === U, i = t === K, r = this._getItemIndex(e), s = this._items.length - 1; if ((i && 0 === r || n && r === s) && !this._config.wrap) return e; var o = (r + (t === K ? -1 : 1)) % this._items.length; return -1 === o ? this._items[this._items.length - 1] : this._items[o] }, t._triggerSlideEvent = function (t, e) { var n = this._getItemIndex(t), i = this._getItemIndex(P(this._element).find(Z.ACTIVE_ITEM)[0]), r = P.Event(Q.SLIDE, { relatedTarget: t, direction: e, from: i, to: n }); return P(this._element).trigger(r), r }, t._setActiveIndicatorElement = function (t) { if (this._indicatorsElement) { P(this._indicatorsElement).find(Z.ACTIVE).removeClass(Y); var e = this._indicatorsElement.children[this._getItemIndex(t)]; e && P(e).addClass(Y) } }, t._slide = function (t, e) { var n, i, r, s = this, o = P(this._element).find(Z.ACTIVE_ITEM)[0], a = this._getItemIndex(o), l = e || o && this._getItemByDirection(t, o), h = this._getItemIndex(l), c = Boolean(this._interval); if (t === U ? (n = z, i = X, r = F) : (n = q, i = J, r = V), l && P(l).hasClass(Y)) this._isSliding = !1; else if (!this._triggerSlideEvent(l, r).isDefaultPrevented() && o && l) { this._isSliding = !0, c && this.pause(), this._setActiveIndicatorElement(l); var u = P.Event(Q.SLID, { relatedTarget: l, direction: r, from: a, to: h }); if (P(this._element).hasClass(G)) { P(l).addClass(i), Cn.reflow(l), P(o).addClass(n), P(l).addClass(n); var f = Cn.getTransitionDurationFromElement(o); P(o).one(Cn.TRANSITION_END, function () { P(l).removeClass(n + " " + i).addClass(Y), P(o).removeClass(Y + " " + i + " " + n), s._isSliding = !1, setTimeout(function () { return P(s._element).trigger(u) }, 0) }).emulateTransitionEnd(f) } else P(o).removeClass(Y), P(l).addClass(Y), this._isSliding = !1, P(this._element).trigger(u); c && this.cycle() } }, s._jQueryInterface = function (i) { return this.each(function () { var t = P(this).data(j), e = h({}, M, P(this).data()); "object" == typeof i && (e = h({}, e, i)); var n = "string" == typeof i ? i : e.slide; if (t || (t = new s(this, e), P(this).data(j, t)), "number" == typeof i) t.to(i); else if ("string" == typeof n) { if ("undefined" == typeof t[n]) throw new TypeError('No method named "' + n + '"'); t[n]() } else e.interval && (t.pause(), t.cycle()) }) }, s._dataApiClickHandler = function (t) { var e = Cn.getSelectorFromElement(this); if (e) { var n = P(e)[0]; if (n && P(n).hasClass(B)) { var i = h({}, P(n).data(), P(this).data()), r = this.getAttribute("data-slide-to"); r && (i.interval = !1), s._jQueryInterface.call(P(n), i), r && P(n).data(j).to(r), t.preventDefault() } } }, o(s, null, [{ key: "VERSION", get: function () { return "4.1.0" } }, { key: "Default", get: function () { return M } }]), s }(), P(document).on(Q.CLICK_DATA_API, Z.DATA_SLIDE, $._dataApiClickHandler), P(window).on(Q.LOAD_DATA_API, function () { P(Z.DATA_RIDE).each(function () { var t = P(this); $._jQueryInterface.call(t, t.data()) }) }), P.fn[L] = $._jQueryInterface, P.fn[L].Constructor = $, P.fn[L].noConflict = function () { return P.fn[L] = W, $._jQueryInterface }, $), bn = (et = "collapse", it = "." + (nt = "bs.collapse"), rt = (tt = e).fn[et], st = { toggle: !0, parent: "" }, ot = { toggle: "boolean", parent: "(string|element)" }, at = { SHOW: "show" + it, SHOWN: "shown" + it, HIDE: "hide" + it, HIDDEN: "hidden" + it, CLICK_DATA_API: "click" + it + ".data-api" }, lt = "show", ht = "collapse", ct = "collapsing", ut = "collapsed", ft = "width", dt = "height", _t = { ACTIVES: ".show, .collapsing", DATA_TOGGLE: '[data-toggle="collapse"]' }, gt = function () { function a(t, e) { this._isTransitioning = !1, this._element = t, this._config = this._getConfig(e), this._triggerArray = tt.makeArray(tt('[data-toggle="collapse"][href="#' + t.id + '"],[data-toggle="collapse"][data-target="#' + t.id + '"]')); for (var n = tt(_t.DATA_TOGGLE), i = 0; i < n.length; i++) { var r = n[i], s = Cn.getSelectorFromElement(r); null !== s && 0 < tt(s).filter(t).length && (this._selector = s, this._triggerArray.push(r)) } this._parent = this._config.parent ? this._getParent() : null, this._config.parent || this._addAriaAndCollapsedClass(this._element, this._triggerArray), this._config.toggle && this.toggle() } var t = a.prototype; return t.toggle = function () { tt(this._element).hasClass(lt) ? this.hide() : this.show() }, t.show = function () { var t, e, n = this; if (!this._isTransitioning && !tt(this._element).hasClass(lt) && (this._parent && 0 === (t = tt.makeArray(tt(this._parent).find(_t.ACTIVES).filter('[data-parent="' + this._config.parent + '"]'))).length && (t = null), !(t && (e = tt(t).not(this._selector).data(nt)) && e._isTransitioning))) { var i = tt.Event(at.SHOW); if (tt(this._element).trigger(i), !i.isDefaultPrevented()) { t && (a._jQueryInterface.call(tt(t).not(this._selector), "hide"), e || tt(t).data(nt, null)); var r = this._getDimension(); tt(this._element).removeClass(ht).addClass(ct), (this._element.style[r] = 0) < this._triggerArray.length && tt(this._triggerArray).removeClass(ut).attr("aria-expanded", !0), this.setTransitioning(!0); var s = "scroll" + (r[0].toUpperCase() + r.slice(1)), o = Cn.getTransitionDurationFromElement(this._element); tt(this._element).one(Cn.TRANSITION_END, function () { tt(n._element).removeClass(ct).addClass(ht).addClass(lt), n._element.style[r] = "", n.setTransitioning(!1), tt(n._element).trigger(at.SHOWN) }).emulateTransitionEnd(o), this._element.style[r] = this._element[s] + "px" } } }, t.hide = function () { var t = this; if (!this._isTransitioning && tt(this._element).hasClass(lt)) { var e = tt.Event(at.HIDE); if (tt(this._element).trigger(e), !e.isDefaultPrevented()) { var n = this._getDimension(); if (this._element.style[n] = this._element.getBoundingClientRect()[n] + "px", Cn.reflow(this._element), tt(this._element).addClass(ct).removeClass(ht).removeClass(lt), 0 < this._triggerArray.length) for (var i = 0; i < this._triggerArray.length; i++) { var r = this._triggerArray[i], s = Cn.getSelectorFromElement(r); if (null !== s) tt(s).hasClass(lt) || tt(r).addClass(ut).attr("aria-expanded", !1) } this.setTransitioning(!0); this._element.style[n] = ""; var o = Cn.getTransitionDurationFromElement(this._element); tt(this._element).one(Cn.TRANSITION_END, function () { t.setTransitioning(!1), tt(t._element).removeClass(ct).addClass(ht).trigger(at.HIDDEN) }).emulateTransitionEnd(o) } } }, t.setTransitioning = function (t) { this._isTransitioning = t }, t.dispose = function () { tt.removeData(this._element, nt), this._config = null, this._parent = null, this._element = null, this._triggerArray = null, this._isTransitioning = null }, t._getConfig = function (t) { return (t = h({}, st, t)).toggle = Boolean(t.toggle), Cn.typeCheckConfig(et, t, ot), t }, t._getDimension = function () { return tt(this._element).hasClass(ft) ? ft : dt }, t._getParent = function () { var n = this, t = null; Cn.isElement(this._config.parent) ? (t = this._config.parent, "undefined" != typeof this._config.parent.jquery && (t = this._config.parent[0])) : t = tt(this._config.parent)[0]; var e = '[data-toggle="collapse"][data-parent="' + this._config.parent + '"]'; return tt(t).find(e).each(function (t, e) { n._addAriaAndCollapsedClass(a._getTargetFromElement(e), [e]) }), t }, t._addAriaAndCollapsedClass = function (t, e) { if (t) { var n = tt(t).hasClass(lt); 0 < e.length && tt(e).toggleClass(ut, !n).attr("aria-expanded", n) } }, a._getTargetFromElement = function (t) { var e = Cn.getSelectorFromElement(t); return e ? tt(e)[0] : null }, a._jQueryInterface = function (i) { return this.each(function () { var t = tt(this), e = t.data(nt), n = h({}, st, t.data(), "object" == typeof i && i); if (!e && n.toggle && /show|hide/.test(i) && (n.toggle = !1), e || (e = new a(this, n), t.data(nt, e)), "string" == typeof i) { if ("undefined" == typeof e[i]) throw new TypeError('No method named "' + i + '"'); e[i]() } }) }, o(a, null, [{ key: "VERSION", get: function () { return "4.1.0" } }, { key: "Default", get: function () { return st } }]), a }(), tt(document).on(at.CLICK_DATA_API, _t.DATA_TOGGLE, function (t) { "A" === t.currentTarget.tagName && t.preventDefault(); var n = tt(this), e = Cn.getSelectorFromElement(this); tt(e).each(function () { var t = tt(this), e = t.data(nt) ? "toggle" : n.data(); gt._jQueryInterface.call(t, e) }) }), tt.fn[et] = gt._jQueryInterface, tt.fn[et].Constructor = gt, tt.fn[et].noConflict = function () { return tt.fn[et] = rt, gt._jQueryInterface }, gt), Sn = (pt = "dropdown", Et = "." + (vt = "bs.dropdown"), yt = ".data-api", Tt = (mt = e).fn[pt], Ct = new RegExp("38|40|27"), It = { HIDE: "hide" + Et, HIDDEN: "hidden" + Et, SHOW: "show" + Et, SHOWN: "shown" + Et, CLICK: "click" + Et, CLICK_DATA_API: "click" + Et + yt, KEYDOWN_DATA_API: "keydown" + Et + yt, KEYUP_DATA_API: "keyup" + Et + yt }, At = "disabled", Dt = "show", bt = "dropup", St = "dropright", wt = "dropleft", Nt = "dropdown-menu-right", Ot = "position-static", kt = '[data-toggle="dropdown"]', Pt = ".dropdown form", Lt = ".dropdown-menu", jt = ".navbar-nav", Rt = ".dropdown-menu .dropdown-item:not(.disabled):not(:disabled)", Ht = "top-start", Wt = "top-end", Mt = "bottom-start", xt = "bottom-end", Ut = "right-start", Kt = "left-start", Ft = { offset: 0, flip: !0, boundary: "scrollParent", reference: "toggle", display: "dynamic" }, Vt = { offset: "(number|string|function)", flip: "boolean", boundary: "(string|element)", reference: "(string|element)", display: "string" }, Qt = function () { function l(t, e) { this._element = t, this._popper = null, this._config = this._getConfig(e), this._menu = this._getMenuElement(), this._inNavbar = this._detectNavbar(), this._addEventListeners() } var t = l.prototype; return t.toggle = function () { if (!this._element.disabled && !mt(this._element).hasClass(At)) { var t = l._getParentFromElement(this._element), e = mt(this._menu).hasClass(Dt); if (l._clearMenus(), !e) { var n = { relatedTarget: this._element }, i = mt.Event(It.SHOW, n); if (mt(t).trigger(i), !i.isDefaultPrevented()) { if (!this._inNavbar) { if ("undefined" == typeof c) throw new TypeError("Bootstrap dropdown require Popper.js (https://popper.js.org)"); var r = this._element; "parent" === this._config.reference ? r = t : Cn.isElement(this._config.reference) && (r = this._config.reference, "undefined" != typeof this._config.reference.jquery && (r = this._config.reference[0])), "scrollParent" !== this._config.boundary && mt(t).addClass(Ot), this._popper = new c(r, this._menu, this._getPopperConfig()) } "ontouchstart" in document.documentElement && 0 === mt(t).closest(jt).length && mt(document.body).children().on("mouseover", null, mt.noop), this._element.focus(), this._element.setAttribute("aria-expanded", !0), mt(this._menu).toggleClass(Dt), mt(t).toggleClass(Dt).trigger(mt.Event(It.SHOWN, n)) } } } }, t.dispose = function () { mt.removeData(this._element, vt), mt(this._element).off(Et), this._element = null, (this._menu = null) !== this._popper && (this._popper.destroy(), this._popper = null) }, t.update = function () { this._inNavbar = this._detectNavbar(), null !== this._popper && this._popper.scheduleUpdate() }, t._addEventListeners = function () { var e = this; mt(this._element).on(It.CLICK, function (t) { t.preventDefault(), t.stopPropagation(), e.toggle() }) }, t._getConfig = function (t) { return t = h({}, this.constructor.Default, mt(this._element).data(), t), Cn.typeCheckConfig(pt, t, this.constructor.DefaultType), t }, t._getMenuElement = function () { if (!this._menu) { var t = l._getParentFromElement(this._element); this._menu = mt(t).find(Lt)[0] } return this._menu }, t._getPlacement = function () { var t = mt(this._element).parent(), e = Mt; return t.hasClass(bt) ? (e = Ht, mt(this._menu).hasClass(Nt) && (e = Wt)) : t.hasClass(St) ? e = Ut : t.hasClass(wt) ? e = Kt : mt(this._menu).hasClass(Nt) && (e = xt), e }, t._detectNavbar = function () { return 0 < mt(this._element).closest(".navbar").length }, t._getPopperConfig = function () { var e = this, t = {}; "function" == typeof this._config.offset ? t.fn = function (t) { return t.offsets = h({}, t.offsets, e._config.offset(t.offsets) || {}), t } : t.offset = this._config.offset; var n = { placement: this._getPlacement(), modifiers: { offset: t, flip: { enabled: this._config.flip }, preventOverflow: { boundariesElement: this._config.boundary } } }; return "static" === this._config.display && (n.modifiers.applyStyle = { enabled: !1 }), n }, l._jQueryInterface = function (e) { return this.each(function () { var t = mt(this).data(vt); if (t || (t = new l(this, "object" == typeof e ? e : null), mt(this).data(vt, t)), "string" == typeof e) { if ("undefined" == typeof t[e]) throw new TypeError('No method named "' + e + '"'); t[e]() } }) }, l._clearMenus = function (t) { if (!t || 3 !== t.which && ("keyup" !== t.type || 9 === t.which)) for (var e = mt.makeArray(mt(kt)), n = 0; n < e.length; n++) { var i = l._getParentFromElement(e[n]), r = mt(e[n]).data(vt), s = { relatedTarget: e[n] }; if (r) { var o = r._menu; if (mt(i).hasClass(Dt) && !(t && ("click" === t.type && /input|textarea/i.test(t.target.tagName) || "keyup" === t.type && 9 === t.which) && mt.contains(i, t.target))) { var a = mt.Event(It.HIDE, s); mt(i).trigger(a), a.isDefaultPrevented() || ("ontouchstart" in document.documentElement && mt(document.body).children().off("mouseover", null, mt.noop), e[n].setAttribute("aria-expanded", "false"), mt(o).removeClass(Dt), mt(i).removeClass(Dt).trigger(mt.Event(It.HIDDEN, s))) } } } }, l._getParentFromElement = function (t) { var e, n = Cn.getSelectorFromElement(t); return n && (e = mt(n)[0]), e || t.parentNode }, l._dataApiKeydownHandler = function (t) { if ((/input|textarea/i.test(t.target.tagName) ? !(32 === t.which || 27 !== t.which && (40 !== t.which && 38 !== t.which || mt(t.target).closest(Lt).length)) : Ct.test(t.which)) && (t.preventDefault(), t.stopPropagation(), !this.disabled && !mt(this).hasClass(At))) { var e = l._getParentFromElement(this), n = mt(e).hasClass(Dt); if ((n || 27 === t.which && 32 === t.which) && (!n || 27 !== t.which && 32 !== t.which)) { var i = mt(e).find(Rt).get(); if (0 !== i.length) { var r = i.indexOf(t.target); 38 === t.which && 0 < r && r--, 40 === t.which && r < i.length - 1 && r++, r < 0 && (r = 0), i[r].focus() } } else { if (27 === t.which) { var s = mt(e).find(kt)[0]; mt(s).trigger("focus") } mt(this).trigger("click") } } }, o(l, null, [{ key: "VERSION", get: function () { return "4.1.0" } }, { key: "Default", get: function () { return Ft } }, { key: "DefaultType", get: function () { return Vt } }]), l }(), mt(document).on(It.KEYDOWN_DATA_API, kt, Qt._dataApiKeydownHandler).on(It.KEYDOWN_DATA_API, Lt, Qt._dataApiKeydownHandler).on(It.CLICK_DATA_API + " " + It.KEYUP_DATA_API, Qt._clearMenus).on(It.CLICK_DATA_API, kt, function (t) { t.preventDefault(), t.stopPropagation(), Qt._jQueryInterface.call(mt(this), "toggle") }).on(It.CLICK_DATA_API, Pt, function (t) { t.stopPropagation() }), mt.fn[pt] = Qt._jQueryInterface, mt.fn[pt].Constructor = Qt, mt.fn[pt].noConflict = function () { return mt.fn[pt] = Tt, Qt._jQueryInterface }, Qt), wn = (Yt = "modal", qt = "." + (Gt = "bs.modal"), zt = (Bt = e).fn[Yt], Xt = { backdrop: !0, keyboard: !0, focus: !0, show: !0 }, Jt = { backdrop: "(boolean|string)", keyboard: "boolean", focus: "boolean", show: "boolean" }, Zt = { HIDE: "hide" + qt, HIDDEN: "hidden" + qt, SHOW: "show" + qt, SHOWN: "shown" + qt, FOCUSIN: "focusin" + qt, RESIZE: "resize" + qt, CLICK_DISMISS: "click.dismiss" + qt, KEYDOWN_DISMISS: "keydown.dismiss" + qt, MOUSEUP_DISMISS: "mouseup.dismiss" + qt, MOUSEDOWN_DISMISS: "mousedown.dismiss" + qt, CLICK_DATA_API: "click" + qt + ".data-api" }, $t = "modal-scrollbar-measure", te = "modal-backdrop", ee = "modal-open", ne = "fade", ie = "show", re = { DIALOG: ".modal-dialog", DATA_TOGGLE: '[data-toggle="modal"]', DATA_DISMISS: '[data-dismiss="modal"]', FIXED_CONTENT: ".fixed-top, .fixed-bottom, .is-fixed, .sticky-top", STICKY_CONTENT: ".sticky-top", NAVBAR_TOGGLER: ".navbar-toggler" }, se = function () { function r(t, e) { this._config = this._getConfig(e), this._element = t, this._dialog = Bt(t).find(re.DIALOG)[0], this._backdrop = null, this._isShown = !1, this._isBodyOverflowing = !1, this._ignoreBackdropClick = !1, this._scrollbarWidth = 0 } var t = r.prototype; return t.toggle = function (t) { return this._isShown ? this.hide() : this.show(t) }, t.show = function (t) { var e = this; if (!this._isTransitioning && !this._isShown) { Bt(this._element).hasClass(ne) && (this._isTransitioning = !0); var n = Bt.Event(Zt.SHOW, { relatedTarget: t }); Bt(this._element).trigger(n), this._isShown || n.isDefaultPrevented() || (this._isShown = !0, this._checkScrollbar(), this._setScrollbar(), this._adjustDialog(), Bt(document.body).addClass(ee), this._setEscapeEvent(), this._setResizeEvent(), Bt(this._element).on(Zt.CLICK_DISMISS, re.DATA_DISMISS, function (t) { return e.hide(t) }), Bt(this._dialog).on(Zt.MOUSEDOWN_DISMISS, function () { Bt(e._element).one(Zt.MOUSEUP_DISMISS, function (t) { Bt(t.target).is(e._element) && (e._ignoreBackdropClick = !0) }) }), this._showBackdrop(function () { return e._showElement(t) })) } }, t.hide = function (t) { var e = this; if (t && t.preventDefault(), !this._isTransitioning && this._isShown) { var n = Bt.Event(Zt.HIDE); if (Bt(this._element).trigger(n), this._isShown && !n.isDefaultPrevented()) { this._isShown = !1; var i = Bt(this._element).hasClass(ne); if (i && (this._isTransitioning = !0), this._setEscapeEvent(), this._setResizeEvent(), Bt(document).off(Zt.FOCUSIN), Bt(this._element).removeClass(ie), Bt(this._element).off(Zt.CLICK_DISMISS), Bt(this._dialog).off(Zt.MOUSEDOWN_DISMISS), i) { var r = Cn.getTransitionDurationFromElement(this._element); Bt(this._element).one(Cn.TRANSITION_END, function (t) { return e._hideModal(t) }).emulateTransitionEnd(r) } else this._hideModal() } } }, t.dispose = function () { Bt.removeData(this._element, Gt), Bt(window, document, this._element, this._backdrop).off(qt), this._config = null, this._element = null, this._dialog = null, this._backdrop = null, this._isShown = null, this._isBodyOverflowing = null, this._ignoreBackdropClick = null, this._scrollbarWidth = null }, t.handleUpdate = function () { this._adjustDialog() }, t._getConfig = function (t) { return t = h({}, Xt, t), Cn.typeCheckConfig(Yt, t, Jt), t }, t._showElement = function (t) { var e = this, n = Bt(this._element).hasClass(ne); this._element.parentNode && this._element.parentNode.nodeType === Node.ELEMENT_NODE || document.body.appendChild(this._element), this._element.style.display = "block", this._element.removeAttribute("aria-hidden"), this._element.scrollTop = 0, n && Cn.reflow(this._element), Bt(this._element).addClass(ie), this._config.focus && this._enforceFocus(); var i = Bt.Event(Zt.SHOWN, { relatedTarget: t }), r = function () { e._config.focus && e._element.focus(), e._isTransitioning = !1, Bt(e._element).trigger(i) }; if (n) { var s = Cn.getTransitionDurationFromElement(this._element); Bt(this._dialog).one(Cn.TRANSITION_END, r).emulateTransitionEnd(s) } else r() }, t._enforceFocus = function () { var e = this; Bt(document).off(Zt.FOCUSIN).on(Zt.FOCUSIN, function (t) { document !== t.target && e._element !== t.target && 0 === Bt(e._element).has(t.target).length && e._element.focus() }) }, t._setEscapeEvent = function () { var e = this; this._isShown && this._config.keyboard ? Bt(this._element).on(Zt.KEYDOWN_DISMISS, function (t) { 27 === t.which && (t.preventDefault(), e.hide()) }) : this._isShown || Bt(this._element).off(Zt.KEYDOWN_DISMISS) }, t._setResizeEvent = function () { var e = this; this._isShown ? Bt(window).on(Zt.RESIZE, function (t) { return e.handleUpdate(t) }) : Bt(window).off(Zt.RESIZE) }, t._hideModal = function () { var t = this; this._element.style.display = "none", this._element.setAttribute("aria-hidden", !0), this._isTransitioning = !1, this._showBackdrop(function () { Bt(document.body).removeClass(ee), t._resetAdjustments(), t._resetScrollbar(), Bt(t._element).trigger(Zt.HIDDEN) }) }, t._removeBackdrop = function () { this._backdrop && (Bt(this._backdrop).remove(), this._backdrop = null) }, t._showBackdrop = function (t) { var e = this, n = Bt(this._element).hasClass(ne) ? ne : ""; if (this._isShown && this._config.backdrop) { if (this._backdrop = document.createElement("div"), this._backdrop.className = te, n && Bt(this._backdrop).addClass(n), Bt(this._backdrop).appendTo(document.body), Bt(this._element).on(Zt.CLICK_DISMISS, function (t) { e._ignoreBackdropClick ? e._ignoreBackdropClick = !1 : t.target === t.currentTarget && ("static" === e._config.backdrop ? e._element.focus() : e.hide()) }), n && Cn.reflow(this._backdrop), Bt(this._backdrop).addClass(ie), !t) return; if (!n) return void t(); var i = Cn.getTransitionDurationFromElement(this._backdrop); Bt(this._backdrop).one(Cn.TRANSITION_END, t).emulateTransitionEnd(i) } else if (!this._isShown && this._backdrop) { Bt(this._backdrop).removeClass(ie); var r = function () { e._removeBackdrop(), t && t() }; if (Bt(this._element).hasClass(ne)) { var s = Cn.getTransitionDurationFromElement(this._backdrop); Bt(this._backdrop).one(Cn.TRANSITION_END, r).emulateTransitionEnd(s) } else r() } else t && t() }, t._adjustDialog = function () { var t = this._element.scrollHeight > document.documentElement.clientHeight; !this._isBodyOverflowing && t && (this._element.style.paddingLeft = this._scrollbarWidth + "px"), this._isBodyOverflowing && !t && (this._element.style.paddingRight = this._scrollbarWidth + "px") }, t._resetAdjustments = function () { this._element.style.paddingLeft = "", this._element.style.paddingRight = "" }, t._checkScrollbar = function () { var t = document.body.getBoundingClientRect(); this._isBodyOverflowing = t.left + t.right < window.innerWidth, this._scrollbarWidth = this._getScrollbarWidth() }, t._setScrollbar = function () { var r = this; if (this._isBodyOverflowing) { Bt(re.FIXED_CONTENT).each(function (t, e) { var n = Bt(e)[0].style.paddingRight, i = Bt(e).css("padding-right"); Bt(e).data("padding-right", n).css("padding-right", parseFloat(i) + r._scrollbarWidth + "px") }), Bt(re.STICKY_CONTENT).each(function (t, e) { var n = Bt(e)[0].style.marginRight, i = Bt(e).css("margin-right"); Bt(e).data("margin-right", n).css("margin-right", parseFloat(i) - r._scrollbarWidth + "px") }), Bt(re.NAVBAR_TOGGLER).each(function (t, e) { var n = Bt(e)[0].style.marginRight, i = Bt(e).css("margin-right"); Bt(e).data("margin-right", n).css("margin-right", parseFloat(i) + r._scrollbarWidth + "px") }); var t = document.body.style.paddingRight, e = Bt(document.body).css("padding-right"); Bt(document.body).data("padding-right", t).css("padding-right", parseFloat(e) + this._scrollbarWidth + "px") } }, t._resetScrollbar = function () { Bt(re.FIXED_CONTENT).each(function (t, e) { var n = Bt(e).data("padding-right"); "undefined" != typeof n && Bt(e).css("padding-right", n).removeData("padding-right") }), Bt(re.STICKY_CONTENT + ", " + re.NAVBAR_TOGGLER).each(function (t, e) { var n = Bt(e).data("margin-right"); "undefined" != typeof n && Bt(e).css("margin-right", n).removeData("margin-right") }); var t = Bt(document.body).data("padding-right"); "undefined" != typeof t && Bt(document.body).css("padding-right", t).removeData("padding-right") }, t._getScrollbarWidth = function () { var t = document.createElement("div"); t.className = $t, document.body.appendChild(t); var e = t.getBoundingClientRect().width - t.clientWidth; return document.body.removeChild(t), e }, r._jQueryInterface = function (n, i) { return this.each(function () { var t = Bt(this).data(Gt), e = h({}, r.Default, Bt(this).data(), "object" == typeof n && n); if (t || (t = new r(this, e), Bt(this).data(Gt, t)), "string" == typeof n) { if ("undefined" == typeof t[n]) throw new TypeError('No method named "' + n + '"'); t[n](i) } else e.show && t.show(i) }) }, o(r, null, [{ key: "VERSION", get: function () { return "4.1.0" } }, { key: "Default", get: function () { return Xt } }]), r }(), Bt(document).on(Zt.CLICK_DATA_API, re.DATA_TOGGLE, function (t) { var e, n = this, i = Cn.getSelectorFromElement(this); i && (e = Bt(i)[0]); var r = Bt(e).data(Gt) ? "toggle" : h({}, Bt(e).data(), Bt(this).data()); "A" !== this.tagName && "AREA" !== this.tagName || t.preventDefault(); var s = Bt(e).one(Zt.SHOW, function (t) { t.isDefaultPrevented() || s.one(Zt.HIDDEN, function () { Bt(n).is(":visible") && n.focus() }) }); se._jQueryInterface.call(Bt(e), r, this) }), Bt.fn[Yt] = se._jQueryInterface, Bt.fn[Yt].Constructor = se, Bt.fn[Yt].noConflict = function () { return Bt.fn[Yt] = zt, se._jQueryInterface }, se), Nn = (ae = "tooltip", he = "." + (le = "bs.tooltip"), ce = (oe = e).fn[ae], ue = "bs-tooltip", fe = new RegExp("(^|\\s)" + ue + "\\S+", "g"), ge = { animation: !0,template: '
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Create DNA
One Problem of cell extract, is the stability of linear DNA. In escherichia coli, endogenous and exogenous linear DNA are differentiated by specific proteins, such as the recombinase complex RecBCD. It recognizes genomic double strand breaks via a specific sequence of 8 bases called Chi-site and prepares the DNA for homologous repair. However, foreign DNA lacks the Chi-site and is therefore degraded by RecBCD. For experiments in cell extract expensive and inefficient inhibitors are used. Since a knockout of RecBCD is lethal, we chose to modify the enzyme to enable selective depletion after cell lysis. Therefore, we came up with several depletion strategies:
Create Soup
The financial advantage of producing home-made cell extract for in vitro protein expression is virtually reversed by the fact that it is tedious and time-consuming, with huge batch to batch variations of the resulting product. Therefore, our objective is to optimize the protocol for self-made cell extract from E. coli, by simplifying time consuming steps while eliminating the central sources of variation during the preparation process. The focus of our effort is to determine the influence of cultivation conditions on extract quality and optimizing the cell lysis for speed, protein yield and nal product quality. By implementing novel ways of extract characterization, we aim to gain a deeper understanding of cell-free systems.
Create Phages
Generation of a universal platform for phage engineering: Our TX-TL system is superior to conventional bacterial based phage production in the following aspects:
- Assembly of phages in vitro
- Exchange of phage fibers in vitro
- Establishment of homogeneous phage batches
- Reduction of endotoxins within phage batches We will exemplarily engineer a non lytic fluorescent T7 phage, which targets a non primary E. coli strain.