Template:Munich/Phactory
Create DNA
One Problem of cell extract, is the stability of linear DNA. In escherichia coli, endogenous and exogenous linear DNA are differentiated by specific proteins, such as the recombinase complex RecBCD. It recognizes genomic double strand breaks via a specific sequence of 8 bases called Chi-site and prepares the DNA for homologous repair. However, foreign DNA lacks the Chi-site and is therefore degraded by RecBCD. For experiments in cell extract expensive and inefficient inhibitors are used. Since a knockout of RecBCD is lethal, we chose to modify the enzyme to enable selective depletion after cell lysis. Therefore, we came up with several depletion strategies:
Create Soup
The financial advantage of producing home-made cell extract for in vitro protein expression is virtually reversed by the fact that it is tedious and time-consuming, with huge batch to batch variations of the resulting product. Therefore, our objective is to optimize the protocol for self-made cell extract from E. coli, by simplifying time consuming steps while eliminating the central sources of variation during the preparation process. The focus of our effort is to determine the influence of cultivation conditions on extract quality and optimizing the cell lysis for speed, protein yield and nal product quality. By implementing novel ways of extract characterization, we aim to gain a deeper understanding of cell-free systems.
Create Phages
Generation of a universal platform for phage engineering: Our TX-TL system is superior to conventional bacterial based phage production in the following aspects:
- Assembly of phages in vitro
- Exchange of phage fibers in vitro
- Establishment of homogeneous phage batches
- Reduction of endotoxins within phage batches We will exemplarily engineer a non lytic fluorescent T7 phage, which targets a non primary E. coli strain.
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return "4.1.0"
}
}, {
key: "Default",
get: function () {
return st
}
}]), a
}(), tt(document).on(at.CLICK_DATA_API, _t.DATA_TOGGLE, function (t) {
"A" === t.currentTarget.tagName && t.preventDefault();
var n = tt(this),
e = Cn.getSelectorFromElement(this);
tt(e).each(function () {
var t = tt(this),
e = t.data(nt) ? "toggle" : n.data();
gt._jQueryInterface.call(t, e)
})
}), tt.fn[et] = gt._jQueryInterface, tt.fn[et].Constructor = gt, tt.fn[et].noConflict = function () {
return tt.fn[et] = rt, gt._jQueryInterface
}, gt),
Sn = (pt = "dropdown", Et = "." + (vt = "bs.dropdown"), yt = ".data-api", Tt = (mt = e).fn[pt], Ct = new RegExp("38|40|27"), It = {
HIDE: "hide" + Et,
HIDDEN: "hidden" + Et,
SHOW: "show" + Et,
SHOWN: "shown" + Et,
CLICK: "click" + Et,
CLICK_DATA_API: "click" + Et + yt,
KEYDOWN_DATA_API: "keydown" + Et + yt,
KEYUP_DATA_API: "keyup" + Et + yt
}, At = "disabled", Dt = "show", bt = "dropup", St = "dropright", wt = "dropleft", Nt = "dropdown-menu-right", Ot = "position-static", kt = '[data-toggle="dropdown"]', Pt = ".dropdown form", Lt = ".dropdown-menu", jt = ".navbar-nav", Rt = ".dropdown-menu .dropdown-item:not(.disabled):not(:disabled)", Ht = "top-start", Wt = "top-end", Mt = "bottom-start", xt = "bottom-end", Ut = "right-start", Kt = "left-start", Ft = {
offset: 0,
flip: !0,
boundary: "scrollParent",
reference: "toggle",
display: "dynamic"
}, Vt = {
offset: "(number|string|function)",
flip: "boolean",
boundary: "(string|element)",
reference: "(string|element)",
display: "string"
}, Qt = function () {
function l(t, e) {
this._element = t, this._popper = null, this._config = this._getConfig(e), this._menu = this._getMenuElement(), this._inNavbar = this._detectNavbar(), this._addEventListeners()
}
var t = l.prototype;
return t.toggle = function () {
if (!this._element.disabled && !mt(this._element).hasClass(At)) {
var t = l._getParentFromElement(this._element),
e = mt(this._menu).hasClass(Dt);
if (l._clearMenus(), !e) {
var n = {
relatedTarget: this._element
},
i = mt.Event(It.SHOW, n);
if (mt(t).trigger(i), !i.isDefaultPrevented()) {
if (!this._inNavbar) {
if ("undefined" == typeof c) throw new TypeError("Bootstrap dropdown require Popper.js (https://popper.js.org)");
var r = this._element;
"parent" === this._config.reference ? r = t : Cn.isElement(this._config.reference) && (r = this._config.reference, "undefined" != typeof this._config.reference.jquery && (r = this._config.reference[0])), "scrollParent" !== this._config.boundary && mt(t).addClass(Ot), this._popper = new c(r, this._menu, this._getPopperConfig())
}
"ontouchstart" in document.documentElement && 0 === mt(t).closest(jt).length && mt(document.body).children().on("mouseover", null, mt.noop), this._element.focus(), this._element.setAttribute("aria-expanded", !0), mt(this._menu).toggleClass(Dt), mt(t).toggleClass(Dt).trigger(mt.Event(It.SHOWN, n))
}
}
}
}, t.dispose = function () {
mt.removeData(this._element, vt), mt(this._element).off(Et), this._element = null, (this._menu = null) !== this._popper && (this._popper.destroy(), this._popper = null)
}, t.update = function () {
this._inNavbar = this._detectNavbar(), null !== this._popper && this._popper.scheduleUpdate()
}, t._addEventListeners = function () {
var e = this;
mt(this._element).on(It.CLICK, function (t) {
t.preventDefault(), t.stopPropagation(), e.toggle()
})
}, t._getConfig = function (t) {
return t = h({}, this.constructor.Default, mt(this._element).data(), t), Cn.typeCheckConfig(pt, t, this.constructor.DefaultType), t
}, t._getMenuElement = function () {
if (!this._menu) {
var t = l._getParentFromElement(this._element);
this._menu = mt(t).find(Lt)[0]
}
return this._menu
}, t._getPlacement = function () {
var t = mt(this._element).parent(),
e = Mt;
return t.hasClass(bt) ? (e = Ht, mt(this._menu).hasClass(Nt) && (e = Wt)) : t.hasClass(St) ? e = Ut : t.hasClass(wt) ? e = Kt : mt(this._menu).hasClass(Nt) && (e = xt), e
}, t._detectNavbar = function () {
return 0 < mt(this._element).closest(".navbar").length
}, t._getPopperConfig = function () {
var e = this,
t = {};
"function" == typeof this._config.offset ? t.fn = function (t) {
return t.offsets = h({}, t.offsets, e._config.offset(t.offsets) || {}), t
} : t.offset = this._config.offset;
var n = {
placement: this._getPlacement(),
modifiers: {
offset: t,
flip: {
enabled: this._config.flip
},
preventOverflow: {
boundariesElement: this._config.boundary
}
}
};
return "static" === this._config.display && (n.modifiers.applyStyle = {
enabled: !1
}), n
}, l._jQueryInterface = function (e) {
return this.each(function () {
var t = mt(this).data(vt);
if (t || (t = new l(this, "object" == typeof e ? e : null), mt(this).data(vt, t)), "string" == typeof e) {
if ("undefined" == typeof t[e]) throw new TypeError('No method named "' + e + '"');
t[e]()
}
})
}, l._clearMenus = function (t) {
if (!t || 3 !== t.which && ("keyup" !== t.type || 9 === t.which))
for (var e = mt.makeArray(mt(kt)), n = 0; n < e.length; n++) {
var i = l._getParentFromElement(e[n]),
r = mt(e[n]).data(vt),
s = {
relatedTarget: e[n]
};
if (r) {
var o = r._menu;
if (mt(i).hasClass(Dt) && !(t && ("click" === t.type && /input|textarea/i.test(t.target.tagName) || "keyup" === t.type && 9 === t.which) && mt.contains(i, t.target))) {
var a = mt.Event(It.HIDE, s);
mt(i).trigger(a), a.isDefaultPrevented() || ("ontouchstart" in document.documentElement && mt(document.body).children().off("mouseover", null, mt.noop), e[n].setAttribute("aria-expanded", "false"), mt(o).removeClass(Dt), mt(i).removeClass(Dt).trigger(mt.Event(It.HIDDEN, s)))
}
}
}
}, l._getParentFromElement = function (t) {
var e, n = Cn.getSelectorFromElement(t);
return n && (e = mt(n)[0]), e || t.parentNode
}, l._dataApiKeydownHandler = function (t) {
if ((/input|textarea/i.test(t.target.tagName) ? !(32 === t.which || 27 !== t.which && (40 !== t.which && 38 !== t.which || mt(t.target).closest(Lt).length)) : Ct.test(t.which)) && (t.preventDefault(), t.stopPropagation(), !this.disabled && !mt(this).hasClass(At))) {
var e = l._getParentFromElement(this),
n = mt(e).hasClass(Dt);
if ((n || 27 === t.which && 32 === t.which) && (!n || 27 !== t.which && 32 !== t.which)) {
var i = mt(e).find(Rt).get();
if (0 !== i.length) {
var r = i.indexOf(t.target);
38 === t.which && 0 < r && r--, 40 === t.which && r < i.length - 1 && r++, r < 0 && (r = 0), i[r].focus()
}
} else {
if (27 === t.which) {
var s = mt(e).find(kt)[0];
mt(s).trigger("focus")
}
mt(this).trigger("click")
}
}
}, o(l, null, [{
key: "VERSION",
get: function () {
return "4.1.0"
}
}, {
key: "Default",
get: function () {
return Ft
}
}, {
key: "DefaultType",
get: function () {
return Vt
}
}]), l
}(), mt(document).on(It.KEYDOWN_DATA_API, kt, Qt._dataApiKeydownHandler).on(It.KEYDOWN_DATA_API, Lt, Qt._dataApiKeydownHandler).on(It.CLICK_DATA_API + " " + It.KEYUP_DATA_API, Qt._clearMenus).on(It.CLICK_DATA_API, kt, function (t) {
t.preventDefault(), t.stopPropagation(), Qt._jQueryInterface.call(mt(this), "toggle")
}).on(It.CLICK_DATA_API, Pt, function (t) {
t.stopPropagation()
}), mt.fn[pt] = Qt._jQueryInterface, mt.fn[pt].Constructor = Qt, mt.fn[pt].noConflict = function () {
return mt.fn[pt] = Tt, Qt._jQueryInterface
}, Qt),
wn = (Yt = "modal", qt = "." + (Gt = "bs.modal"), zt = (Bt = e).fn[Yt], Xt = {
backdrop: !0,
keyboard: !0,
focus: !0,
show: !0
}, Jt = {
backdrop: "(boolean|string)",
keyboard: "boolean",
focus: "boolean",
show: "boolean"
}, Zt = {
HIDE: "hide" + qt,
HIDDEN: "hidden" + qt,
SHOW: "show" + qt,
SHOWN: "shown" + qt,
FOCUSIN: "focusin" + qt,
RESIZE: "resize" + qt,
CLICK_DISMISS: "click.dismiss" + qt,
KEYDOWN_DISMISS: "keydown.dismiss" + qt,
MOUSEUP_DISMISS: "mouseup.dismiss" + qt,
MOUSEDOWN_DISMISS: "mousedown.dismiss" + qt,
CLICK_DATA_API: "click" + qt + ".data-api"
}, $t = "modal-scrollbar-measure", te = "modal-backdrop", ee = "modal-open", ne = "fade", ie = "show", re = {
DIALOG: ".modal-dialog",
DATA_TOGGLE: '[data-toggle="modal"]',
DATA_DISMISS: '[data-dismiss="modal"]',
FIXED_CONTENT: ".fixed-top, .fixed-bottom, .is-fixed, .sticky-top",
STICKY_CONTENT: ".sticky-top",
NAVBAR_TOGGLER: ".navbar-toggler"
}, se = function () {
function r(t, e) {
this._config = this._getConfig(e), this._element = t, this._dialog = Bt(t).find(re.DIALOG)[0], this._backdrop = null, this._isShown = !1, this._isBodyOverflowing = !1, this._ignoreBackdropClick = !1, this._scrollbarWidth = 0
}
var t = r.prototype;
return t.toggle = function (t) {
return this._isShown ? this.hide() : this.show(t)
}, t.show = function (t) {
var e = this;
if (!this._isTransitioning && !this._isShown) {
Bt(this._element).hasClass(ne) && (this._isTransitioning = !0);
var n = Bt.Event(Zt.SHOW, {
relatedTarget: t
});
Bt(this._element).trigger(n), this._isShown || n.isDefaultPrevented() || (this._isShown = !0, this._checkScrollbar(), this._setScrollbar(), this._adjustDialog(), Bt(document.body).addClass(ee), this._setEscapeEvent(), this._setResizeEvent(), Bt(this._element).on(Zt.CLICK_DISMISS, re.DATA_DISMISS, function (t) {
return e.hide(t)
}), Bt(this._dialog).on(Zt.MOUSEDOWN_DISMISS, function () {
Bt(e._element).one(Zt.MOUSEUP_DISMISS, function (t) {
Bt(t.target).is(e._element) && (e._ignoreBackdropClick = !0)
})
}), this._showBackdrop(function () {
return e._showElement(t)
}))
}
}, t.hide = function (t) {
var e = this;
if (t && t.preventDefault(), !this._isTransitioning && this._isShown) {
var n = Bt.Event(Zt.HIDE);
if (Bt(this._element).trigger(n), this._isShown && !n.isDefaultPrevented()) {
this._isShown = !1;
var i = Bt(this._element).hasClass(ne);
if (i && (this._isTransitioning = !0), this._setEscapeEvent(), this._setResizeEvent(), Bt(document).off(Zt.FOCUSIN), Bt(this._element).removeClass(ie), Bt(this._element).off(Zt.CLICK_DISMISS), Bt(this._dialog).off(Zt.MOUSEDOWN_DISMISS), i) {
var r = Cn.getTransitionDurationFromElement(this._element);
Bt(this._element).one(Cn.TRANSITION_END, function (t) {
return e._hideModal(t)
}).emulateTransitionEnd(r)
} else this._hideModal()
}
}
}, t.dispose = function () {
Bt.removeData(this._element, Gt), Bt(window, document, this._element, this._backdrop).off(qt), this._config = null, this._element = null, this._dialog = null, this._backdrop = null, this._isShown = null, this._isBodyOverflowing = null, this._ignoreBackdropClick = null, this._scrollbarWidth = null
}, t.handleUpdate = function () {
this._adjustDialog()
}, t._getConfig = function (t) {
return t = h({}, Xt, t), Cn.typeCheckConfig(Yt, t, Jt), t
}, t._showElement = function (t) {
var e = this,
n = Bt(this._element).hasClass(ne);
this._element.parentNode && this._element.parentNode.nodeType === Node.ELEMENT_NODE || document.body.appendChild(this._element), this._element.style.display = "block", this._element.removeAttribute("aria-hidden"), this._element.scrollTop = 0, n && Cn.reflow(this._element), Bt(this._element).addClass(ie), this._config.focus && this._enforceFocus();
var i = Bt.Event(Zt.SHOWN, {
relatedTarget: t
}),
r = function () {
e._config.focus && e._element.focus(), e._isTransitioning = !1, Bt(e._element).trigger(i)
};
if (n) {
var s = Cn.getTransitionDurationFromElement(this._element);
Bt(this._dialog).one(Cn.TRANSITION_END, r).emulateTransitionEnd(s)
} else r()
}, t._enforceFocus = function () {
var e = this;
Bt(document).off(Zt.FOCUSIN).on(Zt.FOCUSIN, function (t) {
document !== t.target && e._element !== t.target && 0 === Bt(e._element).has(t.target).length && e._element.focus()
})
}, t._setEscapeEvent = function () {
var e = this;
this._isShown && this._config.keyboard ? Bt(this._element).on(Zt.KEYDOWN_DISMISS, function (t) {
27 === t.which && (t.preventDefault(), e.hide())
}) : this._isShown || Bt(this._element).off(Zt.KEYDOWN_DISMISS)
}, t._setResizeEvent = function () {
var e = this;
this._isShown ? Bt(window).on(Zt.RESIZE, function (t) {
return e.handleUpdate(t)
}) : Bt(window).off(Zt.RESIZE)
}, t._hideModal = function () {
var t = this;
this._element.style.display = "none", this._element.setAttribute("aria-hidden", !0), this._isTransitioning = !1, this._showBackdrop(function () {
Bt(document.body).removeClass(ee), t._resetAdjustments(), t._resetScrollbar(), Bt(t._element).trigger(Zt.HIDDEN)
})
}, t._removeBackdrop = function () {
this._backdrop && (Bt(this._backdrop).remove(), this._backdrop = null)
}, t._showBackdrop = function (t) {
var e = this,
n = Bt(this._element).hasClass(ne) ? ne : "";
if (this._isShown && this._config.backdrop) {
if (this._backdrop = document.createElement("div"), this._backdrop.className = te, n && Bt(this._backdrop).addClass(n), Bt(this._backdrop).appendTo(document.body), Bt(this._element).on(Zt.CLICK_DISMISS, function (t) {
e._ignoreBackdropClick ? e._ignoreBackdropClick = !1 : t.target === t.currentTarget && ("static" === e._config.backdrop ? e._element.focus() : e.hide())
}), n && Cn.reflow(this._backdrop), Bt(this._backdrop).addClass(ie), !t) return;
if (!n) return void t();
var i = Cn.getTransitionDurationFromElement(this._backdrop);
Bt(this._backdrop).one(Cn.TRANSITION_END, t).emulateTransitionEnd(i)
} else if (!this._isShown && this._backdrop) {
Bt(this._backdrop).removeClass(ie);
var r = function () {
e._removeBackdrop(), t && t()
};
if (Bt(this._element).hasClass(ne)) {
var s = Cn.getTransitionDurationFromElement(this._backdrop);
Bt(this._backdrop).one(Cn.TRANSITION_END, r).emulateTransitionEnd(s)
} else r()
} else t && t()
}, t._adjustDialog = function () {
var t = this._element.scrollHeight > document.documentElement.clientHeight;
!this._isBodyOverflowing && t && (this._element.style.paddingLeft = this._scrollbarWidth + "px"), this._isBodyOverflowing && !t && (this._element.style.paddingRight = this._scrollbarWidth + "px")
}, t._resetAdjustments = function () {
this._element.style.paddingLeft = "", this._element.style.paddingRight = ""
}, t._checkScrollbar = function () {
var t = document.body.getBoundingClientRect();
this._isBodyOverflowing = t.left + t.right < window.innerWidth, this._scrollbarWidth = this._getScrollbarWidth()
}, t._setScrollbar = function () {
var r = this;
if (this._isBodyOverflowing) {
Bt(re.FIXED_CONTENT).each(function (t, e) {
var n = Bt(e)[0].style.paddingRight,
i = Bt(e).css("padding-right");
Bt(e).data("padding-right", n).css("padding-right", parseFloat(i) + r._scrollbarWidth + "px")
}), Bt(re.STICKY_CONTENT).each(function (t, e) {
var n = Bt(e)[0].style.marginRight,
i = Bt(e).css("margin-right");
Bt(e).data("margin-right", n).css("margin-right", parseFloat(i) - r._scrollbarWidth + "px")
}), Bt(re.NAVBAR_TOGGLER).each(function (t, e) {
var n = Bt(e)[0].style.marginRight,
i = Bt(e).css("margin-right");
Bt(e).data("margin-right", n).css("margin-right", parseFloat(i) + r._scrollbarWidth + "px")
});
var t = document.body.style.paddingRight,
e = Bt(document.body).css("padding-right");
Bt(document.body).data("padding-right", t).css("padding-right", parseFloat(e) + this._scrollbarWidth + "px")
}
}, t._resetScrollbar = function () {
Bt(re.FIXED_CONTENT).each(function (t, e) {
var n = Bt(e).data("padding-right");
"undefined" != typeof n && Bt(e).css("padding-right", n).removeData("padding-right")
}), Bt(re.STICKY_CONTENT + ", " + re.NAVBAR_TOGGLER).each(function (t, e) {
var n = Bt(e).data("margin-right");
"undefined" != typeof n && Bt(e).css("margin-right", n).removeData("margin-right")
});
var t = Bt(document.body).data("padding-right");
"undefined" != typeof t && Bt(document.body).css("padding-right", t).removeData("padding-right")
}, t._getScrollbarWidth = function () {
var t = document.createElement("div");
t.className = $t, document.body.appendChild(t);
var e = t.getBoundingClientRect().width - t.clientWidth;
return document.body.removeChild(t), e
}, r._jQueryInterface = function (n, i) {
return this.each(function () {
var t = Bt(this).data(Gt),
e = h({}, r.Default, Bt(this).data(), "object" == typeof n && n);
if (t || (t = new r(this, e), Bt(this).data(Gt, t)), "string" == typeof n) {
if ("undefined" == typeof t[n]) throw new TypeError('No method named "' + n + '"');
t[n](i)
} else e.show && t.show(i)
})
}, o(r, null, [{
key: "VERSION",
get: function () {
return "4.1.0"
}
}, {
key: "Default",
get: function () {
return Xt
}
}]), r
}(), Bt(document).on(Zt.CLICK_DATA_API, re.DATA_TOGGLE, function (t) {
var e, n = this,
i = Cn.getSelectorFromElement(this);
i && (e = Bt(i)[0]);
var r = Bt(e).data(Gt) ? "toggle" : h({}, Bt(e).data(), Bt(this).data());
"A" !== this.tagName && "AREA" !== this.tagName || t.preventDefault();
var s = Bt(e).one(Zt.SHOW, function (t) {
t.isDefaultPrevented() || s.one(Zt.HIDDEN, function () {
Bt(n).is(":visible") && n.focus()
})
});
se._jQueryInterface.call(Bt(e), r, this)
}), Bt.fn[Yt] = se._jQueryInterface, Bt.fn[Yt].Constructor = se, Bt.fn[Yt].noConflict = function () {
return Bt.fn[Yt] = zt, se._jQueryInterface
}, se),
Nn = (ae = "tooltip", he = "." + (le = "bs.tooltip"), ce = (oe = e).fn[ae], ue = "bs-tooltip", fe = new RegExp("(^|\\s)" + ue + "\\S+", "g"), ge = {
animation: !0,
template: '',
trigger: "hover focus",
title: "",
delay: 0,
html: !(_e = {
AUTO: "auto",
TOP: "top",
RIGHT: "right",
BOTTOM: "bottom",
LEFT: "left"
}),
selector: !(de = {
animation: "boolean",
template: "string",
title: "(string|element|function)",
trigger: "string",
delay: "(number|object)",
html: "boolean",
selector: "(string|boolean)",
placement: "(string|function)",
offset: "(number|string)",
container: "(string|element|boolean)",
fallbackPlacement: "(string|array)",
boundary: "(string|element)"
}),
placement: "top",
offset: 0,
container: !1,
fallbackPlacement: "flip",
boundary: "scrollParent"
}, pe = "out", ve = {
HIDE: "hide" + he,
HIDDEN: "hidden" + he,
SHOW: (me = "show") + he,
SHOWN: "shown" + he,
INSERTED: "inserted" + he,
CLICK: "click" + he,
FOCUSIN: "focusin" + he,
FOCUSOUT: "focusout" + he,
MOUSEENTER: "mouseenter" + he,
MOUSELEAVE: "mouseleave" + he
}, Ee = "fade", ye = "show", Te = ".tooltip-inner", Ce = ".arrow", Ie = "hover", Ae = "focus", De = "click", be = "manual", Se = function () {
function i(t, e) {
if ("undefined" == typeof c) throw new TypeError("Bootstrap tooltips require Popper.js (https://popper.js.org)");
this._isEnabled = !0, this._timeout = 0, this._hoverState = "", this._activeTrigger = {}, this._popper = null, this.element = t, this.config = this._getConfig(e), this.tip = null, this._setListeners()
}
var t = i.prototype;
return t.enable = function () {
this._isEnabled = !0
}, t.disable = function () {
this._isEnabled = !1
}, t.toggleEnabled = function () {
this._isEnabled = !this._isEnabled
}, t.toggle = function (t) {
if (this._isEnabled)
if (t) {
var e = this.constructor.DATA_KEY,
n = oe(t.currentTarget).data(e);
n || (n = new this.constructor(t.currentTarget, this._getDelegateConfig()), oe(t.currentTarget).data(e, n)), n._activeTrigger.click = !n._activeTrigger.click, n._isWithActiveTrigger() ? n._enter(null, n) : n._leave(null, n)
} else {
if (oe(this.getTipElement()).hasClass(ye)) return void this._leave(null, this);
this._enter(null, this)
}
}, t.dispose = function () {
clearTimeout(this._timeout), oe.removeData(this.element, this.constructor.DATA_KEY), oe(this.element).off(this.constructor.EVENT_KEY), oe(this.element).closest(".modal").off("hide.bs.modal"), this.tip && oe(this.tip).remove(), this._isEnabled = null, this._timeout = null, this._hoverState = null, (this._activeTrigger = null) !== this._popper && this._popper.destroy(), this._popper = null, this.element = null, this.config = null, this.tip = null
}, t.show = function () {
var e = this;
if ("none" === oe(this.element).css("display")) throw new Error("Please use show on visible elements");
var t = oe.Event(this.constructor.Event.SHOW);
if (this.isWithContent() && this._isEnabled) {
oe(this.element).trigger(t);
var n = oe.contains(this.element.ownerDocument.documentElement, this.element);
if (t.isDefaultPrevented() || !n) return;
var i = this.getTipElement(),
r = Cn.getUID(this.constructor.NAME);
i.setAttribute("id", r), this.element.setAttribute("aria-describedby", r), this.setContent(), this.config.animation && oe(i).addClass(Ee);
var s = "function" == typeof this.config.placement ? this.config.placement.call(this, i, this.element) : this.config.placement,
o = this._getAttachment(s);
this.addAttachmentClass(o);
var a = !1 === this.config.container ? document.body : oe(this.config.container);
oe(i).data(this.constructor.DATA_KEY, this), oe.contains(this.element.ownerDocument.documentElement, this.tip) || oe(i).appendTo(a), oe(this.element).trigger(this.constructor.Event.INSERTED), this._popper = new c(this.element, i, {
placement: o,
modifiers: {
offset: {
offset: this.config.offset
},
flip: {
behavior: this.config.fallbackPlacement
},
arrow: {
element: Ce
},
preventOverflow: {
boundariesElement: this.config.boundary
}
},
onCreate: function (t) {
t.originalPlacement !== t.placement && e._handlePopperPlacementChange(t)
},
onUpdate: function (t) {
e._handlePopperPlacementChange(t)
}
}), oe(i).addClass(ye), "ontouchstart" in document.documentElement && oe(document.body).children().on("mouseover", null, oe.noop);
var l = function () {
e.config.animation && e._fixTransition();
var t = e._hoverState;
e._hoverState = null, oe(e.element).trigger(e.constructor.Event.SHOWN), t === pe && e._leave(null, e)
};
if (oe(this.tip).hasClass(Ee)) {
var h = Cn.getTransitionDurationFromElement(this.tip);
oe(this.tip).one(Cn.TRANSITION_END, l).emulateTransitionEnd(h)
} else l()
}
}, t.hide = function (t) {
var e = this,
n = this.getTipElement(),
i = oe.Event(this.constructor.Event.HIDE),
r = function () {
e._hoverState !== me && n.parentNode && n.parentNode.removeChild(n), e._cleanTipClass(), e.element.removeAttribute("aria-describedby"), oe(e.element).trigger(e.constructor.Event.HIDDEN), null !== e._popper && e._popper.destroy(), t && t()
};
if (oe(this.element).trigger(i), !i.isDefaultPrevented()) {
if (oe(n).removeClass(ye), "ontouchstart" in document.documentElement && oe(document.body).children().off("mouseover", null, oe.noop), this._activeTrigger[De] = !1, this._activeTrigger[Ae] = !1, this._activeTrigger[Ie] = !1, oe(this.tip).hasClass(Ee)) {
var s = Cn.getTransitionDurationFromElement(n);
oe(n).one(Cn.TRANSITION_END, r).emulateTransitionEnd(s)
} else r();
this._hoverState = ""
}
}, t.update = function () {
null !== this._popper && this._popper.scheduleUpdate()
}, t.isWithContent = function () {
return Boolean(this.getTitle())
}, t.addAttachmentClass = function (t) {
oe(this.getTipElement()).addClass(ue + "-" + t)
}, t.getTipElement = function () {
return this.tip = this.tip || oe(this.config.template)[0], this.tip
}, t.setContent = function () {
var t = oe(this.getTipElement());
this.setElementContent(t.find(Te), this.getTitle()), t.removeClass(Ee + " " + ye)
}, t.setElementContent = function (t, e) {
var n = this.config.html;
"object" == typeof e && (e.nodeType || e.jquery) ? n ? oe(e).parent().is(t) || t.empty().append(e) : t.text(oe(e).text()) : t[n ? "html" : "text"](e)
}, t.getTitle = function () {
var t = this.element.getAttribute("data-original-title");
return t || (t = "function" == typeof this.config.title ? this.config.title.call(this.element) : this.config.title), t
}, t._getAttachment = function (t) {
return _e[t.toUpperCase()]
}, t._setListeners = function () {
var i = this;
this.config.trigger.split(" ").forEach(function (t) {
if ("click" === t) oe(i.element).on(i.constructor.Event.CLICK, i.config.selector, function (t) {
return i.toggle(t)
});
else if (t !== be) {
var e = t === Ie ? i.constructor.Event.MOUSEENTER : i.constructor.Event.FOCUSIN,
n = t === Ie ? i.constructor.Event.MOUSELEAVE : i.constructor.Event.FOCUSOUT;
oe(i.element).on(e, i.config.selector, function (t) {
return i._enter(t)
}).on(n, i.config.selector, function (t) {
return i._leave(t)
})
}
oe(i.element).closest(".modal").on("hide.bs.modal", function () {
return i.hide()
})
}), this.config.selector ? this.config = h({}, this.config, {
trigger: "manual",
selector: ""
}) : this._fixTitle()
}, t._fixTitle = function () {
var t = typeof this.element.getAttribute("data-original-title");
(this.element.getAttribute("title") || "string" !== t) && (this.element.setAttribute("data-original-title", this.element.getAttribute("title") || ""), this.element.setAttribute("title", ""))
}, t._enter = function (t, e) {
var n = this.constructor.DATA_KEY;
(e = e || oe(t.currentTarget).data(n)) || (e = new this.constructor(t.currentTarget, this._getDelegateConfig()), oe(t.currentTarget).data(n, e)), t && (e._activeTrigger["focusin" === t.type ? Ae : Ie] = !0), oe(e.getTipElement()).hasClass(ye) || e._hoverState === me ? e._hoverState = me : (clearTimeout(e._timeout), e._hoverState = me, e.config.delay && e.config.delay.show ? e._timeout = setTimeout(function () {
e._hoverState === me && e.show()
}, e.config.delay.show) : e.show())
}, t._leave = function (t, e) {
var n = this.constructor.DATA_KEY;
(e = e || oe(t.currentTarget).data(n)) || (e = new this.constructor(t.currentTarget, this._getDelegateConfig()), oe(t.currentTarget).data(n, e)), t && (e._activeTrigger["focusout" === t.type ? Ae : Ie] = !1), e._isWithActiveTrigger() || (clearTimeout(e._timeout), e._hoverState = pe, e.config.delay && e.config.delay.hide ? e._timeout = setTimeout(function () {
e._hoverState === pe && e.hide()
}, e.config.delay.hide) : e.hide())
}, t._isWithActiveTrigger = function () {
for (var t in this._activeTrigger)
if (this._activeTrigger[t]) return !0;
return !1
}, t._getConfig = function (t) {
return "number" == typeof (t = h({}, this.constructor.Default, oe(this.element).data(), t)).delay && (t.delay = {
show: t.delay,
hide: t.delay
}), "number" == typeof t.title && (t.title = t.title.toString()), "number" == typeof t.content && (t.content = t.content.toString()), Cn.typeCheckConfig(ae, t, this.constructor.DefaultType), t
}, t._getDelegateConfig = function () {
var t = {};
if (this.config)
for (var e in this.config) this.constructor.Default[e] !== this.config[e] && (t[e] = this.config[e]);
return t
}, t._cleanTipClass = function () {
var t = oe(this.getTipElement()),
e = t.attr("class").match(fe);
null !== e && 0 < e.length && t.removeClass(e.join(""))
}, t._handlePopperPlacementChange = function (t) {
this._cleanTipClass(), this.addAttachmentClass(this._getAttachment(t.placement))
}, t._fixTransition = function () {
var t = this.getTipElement(),
e = this.config.animation;
null === t.getAttribute("x-placement") && (oe(t).removeClass(Ee), this.config.animation = !1, this.hide(), this.show(), this.config.animation = e)
}, i._jQueryInterface = function (n) {
return this.each(function () {
var t = oe(this).data(le),
e = "object" == typeof n && n;
if ((t || !/dispose|hide/.test(n)) && (t || (t = new i(this, e), oe(this).data(le, t)), "string" == typeof n)) {
if ("undefined" == typeof t[n]) throw new TypeError('No method named "' + n + '"');
t[n]()
}
})
}, o(i, null, [{
key: "VERSION",
get: function () {
return "4.1.0"
}
}, {
key: "Default",
get: function () {
return ge
}
}, {
key: "NAME",
get: function () {
return ae
}
}, {
key: "DATA_KEY",
get: function () {
return le
}
}, {
key: "Event",
get: function () {
return ve
}
}, {
key: "EVENT_KEY",
get: function () {
return he
}
}, {
key: "DefaultType",
get: function () {
return de
}
}]), i
}(), oe.fn[ae] = Se._jQueryInterface, oe.fn[ae].Constructor = Se, oe.fn[ae].noConflict = function () {
return oe.fn[ae] = ce, Se._jQueryInterface
}, Se),
On = (Ne = "popover", ke = "." + (Oe = "bs.popover"), Pe = (we = e).fn[Ne], Le = "bs-popover", je = new RegExp("(^|\\s)" + Le + "\\S+", "g"), Re = h({}, Nn.Default, {
placement: "right",
trigger: "click",
content: "",
template: ' }), He = h({}, Nn.DefaultType, {
content: "(string|element|function)"
}), We = "fade", xe = ".popover-header", Ue = ".popover-body", Ke = {
HIDE: "hide" + ke,
HIDDEN: "hidden" + ke,
SHOW: (Me = "show") + ke,
SHOWN: "shown" + ke,
INSERTED: "inserted" + ke,
CLICK: "click" + ke,
FOCUSIN: "focusin" + ke,
FOCUSOUT: "focusout" + ke,
MOUSEENTER: "mouseenter" + ke,
MOUSELEAVE: "mouseleave" + ke
}, Fe = function (t) {
var e, n;
function i() {
return t.apply(this, arguments) || this
}
n = t, (e = i).prototype = Object.create(n.prototype), (e.prototype.constructor = e).__proto__ = n;
var r = i.prototype;
return r.isWithContent = function () {
return this.getTitle() || this._getContent()
}, r.addAttachmentClass = function (t) {
we(this.getTipElement()).addClass(Le + "-" + t)
}, r.getTipElement = function () {
return this.tip = this.tip || we(this.config.template)[0], this.tip
}, r.setContent = function () {
var t = we(this.getTipElement());
this.setElementContent(t.find(xe), this.getTitle());
var e = this._getContent();
"function" == typeof e && (e = e.call(this.element)), this.setElementContent(t.find(Ue), e), t.removeClass(We + " " + Me)
}, r._getContent = function () {
return this.element.getAttribute("data-content") || this.config.content
}, r._cleanTipClass = function () {
var t = we(this.getTipElement()),
e = t.attr("class").match(je);
null !== e && 0 < e.length && t.removeClass(e.join(""))
}, i._jQueryInterface = function (n) {
return this.each(function () {
var t = we(this).data(Oe),
e = "object" == typeof n ? n : null;
if ((t || !/destroy|hide/.test(n)) && (t || (t = new i(this, e), we(this).data(Oe, t)), "string" == typeof n)) {
if ("undefined" == typeof t[n]) throw new TypeError('No method named "' + n + '"');
t[n]()
}
})
}, o(i, null, [{
key: "VERSION",
get: function () {
return "4.1.0"
}
}, {
key: "Default",
get: function () {
return Re
}
}, {
key: "NAME",
get: function () {
return Ne
}
}, {
key: "DATA_KEY",
get: function () {
return Oe
}
}, {
key: "Event",
get: function () {
return Ke
}
}, {
key: "EVENT_KEY",
get: function () {
return ke
}
}, {
key: "DefaultType",
get: function () {
return He
}
}]), i
}(Nn), we.fn[Ne] = Fe._jQueryInterface, we.fn[Ne].Constructor = Fe, we.fn[Ne].noConflict = function () {
return we.fn[Ne] = Pe, Fe._jQueryInterface
}, Fe),
kn = (Qe = "scrollspy", Ye = "." + (Be = "bs.scrollspy"), Ge = (Ve = e).fn[Qe], qe = {
offset: 10,
method: "auto",
target: ""
}, ze = {
offset: "number",
method: "string",
target: "(string|element)"
}, Xe = {
ACTIVATE: "activate" + Ye,
SCROLL: "scroll" + Ye,
LOAD_DATA_API: "load" + Ye + ".data-api"
}, Je = "dropdown-item", Ze = "active", $e = {
DATA_SPY: '[data-spy="scroll"]',
ACTIVE: ".active",
NAV_LIST_GROUP: ".nav, .list-group",
NAV_LINKS: ".nav-link",
NAV_ITEMS: ".nav-item",
LIST_ITEMS: ".list-group-item",
DROPDOWN: ".dropdown",
DROPDOWN_ITEMS: ".dropdown-item",
DROPDOWN_TOGGLE: ".dropdown-toggle"
}, tn = "offset", en = "position", nn = function () {
function n(t, e) {
var n = this;
this._element = t, this._scrollElement = "BODY" === t.tagName ? window : t, this._config = this._getConfig(e), this._selector = this._config.target + " " + $e.NAV_LINKS + "," + this._config.target + " " + $e.LIST_ITEMS + "," + this._config.target + " " + $e.DROPDOWN_ITEMS, this._offsets = [], this._targets = [], this._activeTarget = null, this._scrollHeight = 0, Ve(this._scrollElement).on(Xe.SCROLL, function (t) {
return n._process(t)
}), this.refresh(), this._process()
}
var t = n.prototype;
return t.refresh = function () {
var e = this,
t = this._scrollElement === this._scrollElement.window ? tn : en,
r = "auto" === this._config.method ? t : this._config.method,
s = r === en ? this._getScrollTop() : 0;
this._offsets = [], this._targets = [], this._scrollHeight = this._getScrollHeight(), Ve.makeArray(Ve(this._selector)).map(function (t) {
var e, n = Cn.getSelectorFromElement(t);
if (n && (e = Ve(n)[0]), e) {
var i = e.getBoundingClientRect();
if (i.width || i.height) return [Ve(e)[r]().top + s, n]
}
return null
}).filter(function (t) {
return t
}).sort(function (t, e) {
return t[0] - e[0]
}).forEach(function (t) {
e._offsets.push(t[0]), e._targets.push(t[1])
})
}, t.dispose = function () {
Ve.removeData(this._element, Be), Ve(this._scrollElement).off(Ye), this._element = null, this._scrollElement = null, this._config = null, this._selector = null, this._offsets = null, this._targets = null, this._activeTarget = null, this._scrollHeight = null
}, t._getConfig = function (t) {
if ("string" != typeof (t = h({}, qe, t)).target) {
var e = Ve(t.target).attr("id");
e || (e = Cn.getUID(Qe), Ve(t.target).attr("id", e)), t.target = "#" + e
}
return Cn.typeCheckConfig(Qe, t, ze), t
}, t._getScrollTop = function () {
return this._scrollElement === window ? this._scrollElement.pageYOffset : this._scrollElement.scrollTop
}, t._getScrollHeight = function () {
return this._scrollElement.scrollHeight || Math.max(document.body.scrollHeight, document.documentElement.scrollHeight)
}, t._getOffsetHeight = function () {
return this._scrollElement === window ? window.innerHeight : this._scrollElement.getBoundingClientRect().height
}, t._process = function () {
var t = this._getScrollTop() + this._config.offset,
e = this._getScrollHeight(),
n = this._config.offset + e - this._getOffsetHeight();
if (this._scrollHeight !== e && this.refresh(), n <= t) {
var i = this._targets[this._targets.length - 1];
this._activeTarget !== i && this._activate(i)
} else {
if (this._activeTarget && t < this._offsets[0] && 0 < this._offsets[0]) return this._activeTarget = null, void this._clear();
for (var r = this._offsets.length; r--;) {
this._activeTarget !== this._targets[r] && t >= this._offsets[r] && ("undefined" == typeof this._offsets[r + 1] || t < this._offsets[r + 1]) && this._activate(this._targets[r])
}
}
}, t._activate = function (e) {
this._activeTarget = e, this._clear();
var t = this._selector.split(",");
t = t.map(function (t) {
return t + '[data-target="' + e + '"],' + t + '[href="' + e + '"]'
});
var n = Ve(t.join(","));
n.hasClass(Je) ? (n.closest($e.DROPDOWN).find($e.DROPDOWN_TOGGLE).addClass(Ze), n.addClass(Ze)) : (n.addClass(Ze), n.parents($e.NAV_LIST_GROUP).prev($e.NAV_LINKS + ", " + $e.LIST_ITEMS).addClass(Ze), n.parents($e.NAV_LIST_GROUP).prev($e.NAV_ITEMS).children($e.NAV_LINKS).addClass(Ze)), Ve(this._scrollElement).trigger(Xe.ACTIVATE, {
relatedTarget: e
})
}, t._clear = function () {
Ve(this._selector).filter($e.ACTIVE).removeClass(Ze)
}, n._jQueryInterface = function (e) {
return this.each(function () {
var t = Ve(this).data(Be);
if (t || (t = new n(this, "object" == typeof e && e), Ve(this).data(Be, t)), "string" == typeof e) {
if ("undefined" == typeof t[e]) throw new TypeError('No method named "' + e + '"');
t[e]()
}
})
}, o(n, null, [{
key: "VERSION",
get: function () {
return "4.1.0"
}
}, {
key: "Default",
get: function () {
return qe
}
}]), n
}(), Ve(window).on(Xe.LOAD_DATA_API, function () {
for (var t = Ve.makeArray(Ve($e.DATA_SPY)), e = t.length; e--;) {
var n = Ve(t[e]);
nn._jQueryInterface.call(n, n.data())
}
}), Ve.fn[Qe] = nn._jQueryInterface, Ve.fn[Qe].Constructor = nn, Ve.fn[Qe].noConflict = function () {
return Ve.fn[Qe] = Ge, nn._jQueryInterface
}, nn),
Pn = (on = "." + (sn = "bs.tab"), an = (rn = e).fn.tab, ln = {
HIDE: "hide" + on,
HIDDEN: "hidden" + on,
SHOW: "show" + on,
SHOWN: "shown" + on,
CLICK_DATA_API: "click" + on + ".data-api"
}, hn = "dropdown-menu", cn = "active", un = "disabled", fn = "fade", dn = "show", _n = ".dropdown", gn = ".nav, .list-group", mn = ".active", pn = "> li > .active", vn = '[data-toggle="tab"], [data-toggle="pill"], [data-toggle="list"]', En = ".dropdown-toggle", yn = "> .dropdown-menu .active", Tn = function () {
function i(t) {
this._element = t
}
var t = i.prototype;
return t.show = function () {
var n = this;
if (!(this._element.parentNode && this._element.parentNode.nodeType === Node.ELEMENT_NODE && rn(this._element).hasClass(cn) || rn(this._element).hasClass(un))) {
var t, i, e = rn(this._element).closest(gn)[0],
r = Cn.getSelectorFromElement(this._element);
if (e) {
var s = "UL" === e.nodeName ? pn : mn;
i = (i = rn.makeArray(rn(e).find(s)))[i.length - 1]
}
var o = rn.Event(ln.HIDE, {
relatedTarget: this._element
}),
a = rn.Event(ln.SHOW, {
relatedTarget: i
});
if (i && rn(i).trigger(o), rn(this._element).trigger(a), !a.isDefaultPrevented() && !o.isDefaultPrevented()) {
r && (t = rn(r)[0]), this._activate(this._element, e);
var l = function () {
var t = rn.Event(ln.HIDDEN, {
relatedTarget: n._element
}),
e = rn.Event(ln.SHOWN, {
relatedTarget: i
});
rn(i).trigger(t), rn(n._element).trigger(e)
};
t ? this._activate(t, t.parentNode, l) : l()
}
}
}, t.dispose = function () {
rn.removeData(this._element, sn), this._element = null
}, t._activate = function (t, e, n) {
var i = this,
r = ("UL" === e.nodeName ? rn(e).find(pn) : rn(e).children(mn))[0],
s = n && r && rn(r).hasClass(fn),
o = function () {
return i._transitionComplete(t, r, n)
};
if (r && s) {
var a = Cn.getTransitionDurationFromElement(r);
rn(r).one(Cn.TRANSITION_END, o).emulateTransitionEnd(a)
} else o()
}, t._transitionComplete = function (t, e, n) {
if (e) {
rn(e).removeClass(dn + " " + cn);
var i = rn(e.parentNode).find(yn)[0];
i && rn(i).removeClass(cn), "tab" === e.getAttribute("role") && e.setAttribute("aria-selected", !1)
}
if (rn(t).addClass(cn), "tab" === t.getAttribute("role") && t.setAttribute("aria-selected", !0), Cn.reflow(t), rn(t).addClass(dn), t.parentNode && rn(t.parentNode).hasClass(hn)) {
var r = rn(t).closest(_n)[0];
r && rn(r).find(En).addClass(cn), t.setAttribute("aria-expanded", !0)
}
n && n()
}, i._jQueryInterface = function (n) {
return this.each(function () {
var t = rn(this),
e = t.data(sn);
if (e || (e = new i(this), t.data(sn, e)), "string" == typeof n) {
if ("undefined" == typeof e[n]) throw new TypeError('No method named "' + n + '"');
e[n]()
}
})
}, o(i, null, [{
key: "VERSION",
get: function () {
return "4.1.0"
}
}]), i
}(), rn(document).on(ln.CLICK_DATA_API, vn, function (t) {
t.preventDefault(), Tn._jQueryInterface.call(rn(this), "show")
}), rn.fn.tab = Tn._jQueryInterface, rn.fn.tab.Constructor = Tn, rn.fn.tab.noConflict = function () {
return rn.fn.tab = an, Tn._jQueryInterface
}, Tn);
! function (t) {
if ("undefined" == typeof t) throw new TypeError("Bootstrap's JavaScript requires jQuery. jQuery must be included before Bootstrap's JavaScript.");
var e = t.fn.jquery.split(" ")[0].split(".");
if (e[0] < 2 && e[1] < 9 || 1 === e[0] && 9 === e[1] && e[2] < 1 || 4 <= e[0]) throw new Error("Bootstrap's JavaScript requires at least jQuery v1.9.1 but less than v4.0.0")
}(e), t.Util = Cn, t.Alert = In, t.Button = An, t.Carousel = Dn, t.Collapse = bn, t.Dropdown = Sn, t.Modal = wn, t.Popover = On, t.Scrollspy = kn, t.Tab = Pn, t.Tooltip = Nn, Object.defineProperty(t, "__esModule", {
value: !0
})
});