Team:iTesla-SoundBio/Lab Notebook

InterLab Human Practices Collaborations

Recording Our Progress

Date: 2018/06/02
Transform blue coral amilCP into already competent dh5 alpha cells
Date: 2018/06/08
PCR Practice and E. Coli Culturing
Date: 2018/06/09
Miniprep Integration vector and BBa_k608006 (biobricking backbone) for future digestion and ligation. Also, to run an analytical gel on last night’s PCR to analyze its success.
Date: 2018/06/10
Repeat Miniprep of Biobrick Backbone
Date: 2018/06/11
Redo PCR of gblocks and Redo Analytical Gel on First PCR
Date: 2018/06/16
Digest of Miniprepped Biobricking Backbone and Analytical Gel
Date: 2018/06/17
Gel Purifying Digested BB Backbone slices and Digesting PCR'd FC 1
Date: 2018/06/21
Second Miniprep of Biobricking Backbone & Integration Vector of B. Subtilis and testing PCRs with phusion stuff AND test-digest for SacII and BglII
Date: 2018/06/22
Gel Test of psb1a3, pax01, and test-digest for BglII/SacII. BB digest of psb1a3, and purification gel
Date: 2018/06/30
Gel Extraction of psb1A3, Ligation of FC Frag 1 and psb1a3, and Transformation of Ligated Plasmid into E.coli
Date: 2018/07/05
Digesting, running a purification gel and gel extracting miniprepped psb1a3
Date: 2018/07/06
Miniprepping psb1a3 (Round #3)
Date: 2018/07/07
Digesting and gel purifying psb1a3, PCRing Frag 1
Date: 2018/07/12
Gel extracting digested psb1a3, PCRing and PCR purifying Factor C Frag 2
Date: 2018/07/14
Digest psb1a3, gel purify, gel extract and nanodrop digested psb1a3
Date: 2018/07/16
Digesting Frag 1, Ligating Frag 1 and Digested psb1a3, Transforming into E.coli, Transforming Interlab plasmids into E.coli, and Miniprepping psb1a3

Date: 2018/07/20
ESETITTTTTTTTTTTTTTTTTTTTTTTTT
Date: 2018/07/21
PCRing more Fragment 1, Running an analytical gel, digesting psb1a3 and psb1c3, running a purificaiton gel, gel extracting and nanodropping resulting digests
Date: 2018/07/22
Miniprepping psb1a3 and psb1c3, digesting, running a purification gel and gel extracting
Date: 2018/07/27
Digesting Frag 1, Ligating Frag 1 and psb1a3/c3, Transforming into E.coli
Date: 2018/07/29
More PCRs of Fragment 1
Date: 2018/07/30
Miniprep, digest, and gel purification of pSB1C3, pcr and pcr purification of fragment 1 geneblock
Date: 2018/07/31
Miniprepping psb1a3 (Round #3)
Date: 2018/08/01
Running a Purification Gel on Digested FC 1 NOT SUCCESSFUL
Date: 2018/08/02
Purification Gel of psb1C3
Date: 2018/08/03
PCR fragment 1
Date: 2018/08/07
PCR, Digest Fragment 1 & Digest psb1C3
Date: 2018/08/08
Debugging PCR using psb1C3 and DMSO
Date: 2018/08/09
PCR'ing Fragment 2, Digesting psb1C3
Date: 2018/08/10
Gel extraction of digested fragment 2
Date: 2018/08/11
Analytical gel of frag 1 PCRs and gel extraction of fragment 2 digests
Date: 2018/08/12
Ligation of pSB1C3 + frag 2 and transformation
Date: 2018/08/21
Miniprep of pSB1C3+mRFP, PCR of fragment 2 and fragment 1
Date: 2018/08/22
PCR of fragments 1, 2, and pSB1C3 and analytical gel
Date: 2018/08/26
PCR of fragments 1 and 2

Date: 2018/08/27
Practice with bacillus
Date: 2018/08/29
Practice FastClone Transformation
Date: 2018/08/31
Miniprep and PCR of xylose cassette and PCR of pAX01
Date: 2018/09/01
more pcrs.....
Date: 2018/09/02
Analytical gel of PCRs, digest with DpnI, and transformation
Date: 2018/09/08
Transformation of B. subtilis with pAX01 and PCRs of pAX01
Date: 2018/09/09
practice transformation with bacillus
Date: 2018/09/15
Transformation of competent B. subtilis 168
Date: 2018/09/21
More transformations and minipreps of pAX01
Date: 2018/09/22
More transformations with bacillus
Date: 2018/09/23 AM
Transformation Tests
Date: 2018/09/23 PM
Primer Design
Date: 2018/09/28
Buffers and Prep stuff
Date: 2018/09/29
Colony PCR and transformation stuff
Date: 2018/09/30
More colony PCRs
Date: 2018/10/03 AM
Instructions for David about colony PCR
Date: 2018/10/06 PM
analysis of colony pcr results and then...other stuff
Date: 2018/10/07
Leads Meeting
Date: 2018/10/11
Colony PCRs (for integration confirmation and 16s sequencing)
Date: 2018/10/13
Colony PCR for 16s Genome Sequencing