Team:Marburg/Part Collection

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Part Collection

Explanation

Figure 1: Hierarchical cloning is facilitated by subsequent Golden Gate reactions.
Basic building blocks like promoters or terminators are stored in level 0 plasmids. Parts from each category of our collection can be chosen to built level 1 plasmids harboring a single transcription unit. Up to five transcription units can be assembled into a level 2 plasmid.

We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids. 36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk. The Marburg Collection contains 123 parts in total, including: inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.(Range:BBa_K2560001 - BBa_K2560305)

Figure 2: Additional bases and fusion sites ensure correct spacing and allow tags.
Between some parts, additional base pairs were integrated to ensure correct spacing and to maintain the triplet code. We expanded our toolbox by providing N- and C- terminal tags by creating novel fusions and splitting the CDS and terminator part, respectivel

Parts of the Marburg Collection

Download the "Marburg Collection"

5'Connector

Download all 5'Connectors

BioBrick	Name	Plasmid
K2560011	5'Connector Dummy	BBa_K2560002 (pSB1C3 derivative)
K2560055	1-6 Connector	BBa_K2560002 (pSB1C3 derivative)
K2560065	5'Con1	BBa_K2560002 (pSB1C3 derivative)
K2560066	5'Con2	BBa_K2560002 (pSB1C3 derivative)
K2560067	5'Con3	BBa_K2560002 (pSB1C3 derivative)
K2560066	5'Con4	BBa_K2560002 (pSB1C3 derivative)
K2560069	5'Con5	BBa_K2560002 (pSB1C3 derivative)
K2560075	5'Con1 Short Res	BBa_K2560002 (pSB1C3 derivative)
K2560076	5'Con2 Short	BBa_K2560002 (pSB1C3 derivative)
K2560077	5'Con3 Short	BBa_K2560002 (pSB1C3 derivative)
K2560078	5'Con4 Short	BBa_K2560002 (pSB1C3 derivative)
K2560079	5'Con5 Short	BBa_K2560002 (pSB1C3 derivative)
K2560095	5'Con1 inv	BBa_K2560002 (pSB1C3 derivative)
K2560096	5'Con2 inv	BBa_K2560002 (pSB1C3 derivative)
K2560097	5'Con3 inv	BBa_K2560002 (pSB1C3 derivative)
K2560098	5'Con4 inv	BBa_K2560002 (pSB1C3 derivative)
K2560099	5'Con5 inv	BBa_K2560002 (pSB1C3 derivative)
K2560105	5'Con5 inv Ori	BBa_K2560002 (pSB1C3 derivative)
K2560107	5'Con1 Res	BBa_K2560002 (pSB1C3 derivative)

Promoter

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BioBrick	Name	Plasmid
K2560007	J23100	BBa_K2560002 (pSB1C3 derivative)
K2560009	J23104	BBa_K2560002 (pSB1C3 derivative)
K2560014	J23106	BBa_K2560002 (pSB1C3 derivative)
K2560015	J23115	BBa_K2560002 (pSB1C3 derivative)
K2560017	J23101	BBa_K2560002 (pSB1C3 derivative)
K2560018	J23102	BBa_K2560002 (pSB1C3 derivative)
K2560019	J23103	BBa_K2560002 (pSB1C3 derivative)
K2560020	J23105	BBa_K2560002 (pSB1C3 derivative)
K2560021	J23107	BBa_K2560002 (pSB1C3 derivative)
K2560022	J23108	BBa_K2560002 (pSB1C3 derivative)
K2560023	J23109	BBa_K2560002 (pSB1C3 derivative)
K2560024	J23110	BBa_K2560002 (pSB1C3 derivative)
K2560025	J23111	BBa_K2560002 (pSB1C3 derivative)
K2560026	J23113	BBa_K2560002 (pSB1C3 derivative)
K2560027	J23114	BBa_K2560002 (pSB1C3 derivative)
K2560028	J23116	BBa_K2560002 (pSB1C3 derivative)
K2560029	J23117	BBa_K2560002 (pSB1C3 derivative)
K2560029	J23117	BBa_K2560002 (pSB1C3 derivative)
K2560030	J23118	BBa_K2560002 (pSB1C3 derivative)
K2560031	J23119	BBa_K2560002 (pSB1C3 derivative)
K2560123	pTet	BBa_K2560002 (pSB1C3 derivative)
K2560124	pTrc	BBa_K2560002 (pSB1C3 derivative)
K2560131	Promoter Dummy	BBa_K2560002 (pSB1C3 derivative)

RBS

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BioBrick	Name	Plasmid
K2560008	B0034	BBa_K2560002 (pSB1C3 derivative)
K2560010	B0032	BBa_K2560002 (pSB1C3 derivative)
K2560013	B0031	BBa_K2560002 (pSB1C3 derivative)
K2560016	B0030	BBa_K2560002 (pSB1C3 derivative)
K2560084	RBS Dummy	BBa_K2560002 (pSB1C3 derivative)

CDS

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BioBrick	Name	Plasmid
K2560033	E1010	BBa_K2560002 (pSB1C3 derivative)
K2560041	E0030	BBa_K2560002 (pSB1C3 derivative)
K2560042	sfGFP	BBa_K2560002 (pSB1C3 derivative)
K2560043	sfGFP Vn	BBa_K2560002 (pSB1C3 derivative)
K2560047	Cas9	BBa_K2560002 (pSB1C3 derivative)
K2560051	Lux Operon	BBa_K2560002 (pSB1C3 derivative)
K2560054	dCas9	BBa_K2560002 (pSB1C3 derivative)
K2560082	CDS Dummy	BBa_K2560002 (pSB1C3 derivative)
K2560114	LacZ Alpha	BBa_K2560002 (pSB1C3 derivative)
K2560128	K6600004	BBa_K2560002 (pSB1C3 derivative)
K2560135	SXT Beta	BBa_K2560002 (pSB1C3 derivative)
K2560268	tfox Vc	BBa_K2560002 (pSB1C3 derivative)
K2560269	tfox Vn	BBa_K2560002 (pSB1C3 derivative)
K2560270	SXT	BBa_K2560002 (pSB1C3 derivative)
K2560272	flp recombinase	BBa_K2560002 (pSB1C3 derivative)

Terminator

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BioBrick	Name	Plasmid
K2560034	B0010	BBa_K2560002 (pSB1C3 derivative)
K2560035	B0015	BBa_K2560002 (pSB1C3 derivative)
K2560081	Terminator Dummy	BBa_K2560002 (pSB1C3 derivative)
K2560089	B1002	BBa_K2560002 (pSB1C3 derivative)
K2560091	B0003	BBa_K2560002 (pSB1C3 derivative)
K2560093	B0006	BBa_K2560002 (pSB1C3 derivative)

3'Connector

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BioBrick	Name	Plasmid
K2560012	3'Connector Dummy	BBa_K2560002 (pSB1C3 derivative)
K2560070	3'Con1	BBa_K2560002 (pSB1C3 derivative)
K2560071	3'Con2	BBa_K2560002 (pSB1C3 derivative)
K2560072	3'Con3	BBa_K2560002 (pSB1C3 derivative)
K2560073	3'Con4	BBa_K2560002 (pSB1C3 derivative)
K2560080	3'Con5 Ori	BBa_K2560002 (pSB1C3 derivative)
K2560100	3'Con1 inv Short	BBa_K2560002 (pSB1C3 derivative)
K2560101	3'Con2 inv Short	BBa_K2560002 (pSB1C3 derivative)
K2560102	3'Con3 inv Short	BBa_K2560002 (pSB1C3 derivative)
K2560103	3'Con4 inv Short	BBa_K2560002 (pSB1C3 derivative)
K2560104	3'Con5 inv Short	BBa_K2560002 (pSB1C3 derivative)
K2560106	3'Con1 inv Res	BBa_K2560002 (pSB1C3 derivative)
K2560108	3'Con1 inv	BBa_K2560002 (pSB1C3 derivative)
K2560109	3'Con1 inv Res	BBa_K2560002 (pSB1C3 derivative)
K2560110	3'Con2 inv	BBa_K2560002 (pSB1C3 derivative)
K2560111	3'Con3 inv	BBa_K2560002 (pSB1C3 derivative)
K2560112	3'Con4 inv	BBa_K2560002 (pSB1C3 derivative)
K2560113	3'Con5 inv	BBa_K2560002 (pSB1C3 derivative)

ORI

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BioBrick	Name	Plasmid
K2560036	ColE1	BBa_K2560002 (pSB1C3 derivative)
K2560037	pMB1	BBa_K2560002 (pSB1C3 derivative)
K2560046	p15A	BBa_K2560002 (pSB1C3 derivative)

Antibiotic Resistance

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BioBrick	Name	Plasmid
K2560048	Cam. Res. RFP	BBa_K2560002 (pSB1C3 derivative)
K2560056	Kan. Res.(pSB3K3) RFP	BBa_K2560002 (pSB1C3 derivative)
K2560057	Kan. Res. (pSB3K3) GFP	BBa_K2560002 (pSB1C3 derivative)
K2560058	Tet. Res. (pSB3T5) RFP	BBa_K2560002 (pSB1C3 derivative)
K2560059	Tet. Res. (pSB3T5) GFP	BBa_K2560002 (pSB1C3 derivative)
K2560125	Carb. Res. RFP	BBa_K2560002 (pSB1C3 derivative)
K2560126	Carb. Res. GFP	BBa_K2560002 (pSB1C3 derivative)
K2560127	Carb. Res. into BBa_K2560002	BBa_K2560002 (pSB1C3 derivative)
K2560132	Cam. Res. into BBa_K2560002	BBa_K2560002 (pSB1C3 derivative)
K2560133	Kan. Res. into BBa_K2560002	BBa_K2560002 (pSB1C3 derivative)
K2560134	Tet. Res. into BBa_K2560002	BBa_K2560002 (pSB1C3 derivative)

N-Tag

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BioBrick	Name	Plasmid
K2560060	E1010 4x	BBa_K2560002 (pSB1C3 derivative)
K2560063	sfGFP 4x	BBa_K2560002 (pSB1C3 derivative)
K2560085	His Tag 4x	BBa_K2560002 (pSB1C3 derivative)
K2560087	FLAG Tag 4x	BBa_K2560002 (pSB1C3 derivative)
K2560129	K660004 4x	BBa_K2560002 (pSB1C3 derivative)
K2560257	Streptag 4x	BBa_K2560002 (pSB1C3 derivative)

C-Tag

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BioBrick	Name	Plasmid
K2560061	E1010 5a	BBa_K2560002 (pSB1C3 derivative)
K2560062	B0015 5a	BBa_K2560002 (pSB1C3 derivative)
K2560064	sfGFP 5a	BBa_K2560002 (pSB1C3 derivative)
K2560086	His Tag 5a	BBa_K2560002 (pSB1C3 derivative)
K2560088	FLAG Tag 5a	BBa_K2560002 (pSB1C3 derivative)
K2560090	B1002 5b	BBa_K2560002 (pSB1C3 derivative)
K2560092	B1003 5b	BBa_K2560002 (pSB1C3 derivative)
K2560094	B1006 5b	BBa_K2560002 (pSB1C3 derivative)
K2560117	I11012 5a	BBa_K2560002 (pSB1C3 derivative)
K2560118	M0050 5a	BBa_K2560002 (pSB1C3 derivative)
K2560119	M0051 5a	BBa_K2560002 (pSB1C3 derivative)
K2560130	K660004 5a	BBa_K2560002 (pSB1C3 derivative)
K2560258	Streptag 5a	BBa_K2560002 (pSB1C3 derivative)

Entry Vector

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BioBrick	Name	Plasmid
K2560001	Entry Vector with RFP Dropout	BBa_K2560002 (pSB1C3 derivative)
K2560002	Entry Vector with GFP Dropout	BBa_K2560002 (pSB1C3 derivative)
K2560005	Resistance Entry Vector with RFP Dropout	Falsch
K2560006	Resistance Entry Vector with GFP Dropout	Falsch
K2560305	gRNA Entry Vector with GFP Dropout	BBa_K2560002 (pSB1C3 derivative)